Impairment of hepatic nuclear factor-4α binding to the stim1 promoter contributes to high glucose-induced upregulation of stim1 expression in glomerular mesangial cells

Yanxia Wang, Sarika Chaudhari, Yuezhong Ren, Rong Ma

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4 Citations (Scopus)

Abstract

The present study was carried out to investigate if hepatic nuclear factor (HNF)4α contributed to the high glucose-induced increase in stromal interacting molecule (STIM)1 protein abundance in glomerular mesangial cells (MCs). Western blot and immunofluorescence experiments showed HNF4α expression in MCs. Knockdown of HNF4α using a small interfering RNA approach significantly increased mRNA expression levels of both STIM1 and Orai1 and protein expression levels of STIM1 in cultured human MCs. Consistently, overexpression of HNF4α reduced expressed STIM1 protein expression in human embryonic kidney-293 cells. Furthermore, high glucose treatment did not significantly change the abundance of HNF4α protein in MCs but significantly attenuated HNF4α binding activity to the Stim1 promoter. Moreover, knockdown of HNF4α significantly augmented store-operated Ca2+ entry, which is known to be gated by STIM1 and has recently been found to be antifibrotic in MCs. In agreement with those results, knockdown of HNF4[1] significantly attenuated the fibrotic response of high glucose. These results suggest that HNF4[1] negatively regulates STIM1 transcription in MCs. High glucose increases STIM1 expression levels by impairing HNF4α binding activity to the Stim1 promoter, which subsequently releases Stim1 transcription from HNF4α repression. Since the STIM1-gated storeoperated Ca2+ entry pathway in MCs has an antifibrotic effect, inhibition of HNF4α in MCs might be a potential therapeutic option for diabetic kidney disease.

Original languageEnglish
Pages (from-to)F1135-F1145
JournalAmerican Journal of Physiology - Renal Physiology
Volume308
Issue number10
DOIs
StatePublished - 15 May 2015

Fingerprint

Mesangial Cells
Up-Regulation
Glucose
Liver
Diabetic Nephropathies
Small Interfering RNA
Fluorescent Antibody Technique
Proteins
Western Blotting
Kidney
Messenger RNA
Therapeutics

Keywords

  • Diabetic nephropathy
  • Hepatic nuclear factor 4α
  • High glucose
  • Mesangial cells
  • Storeoperated Ca entry
  • Stromal interacting molecule 1

Cite this

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title = "Impairment of hepatic nuclear factor-4α binding to the stim1 promoter contributes to high glucose-induced upregulation of stim1 expression in glomerular mesangial cells",
abstract = "The present study was carried out to investigate if hepatic nuclear factor (HNF)4α contributed to the high glucose-induced increase in stromal interacting molecule (STIM)1 protein abundance in glomerular mesangial cells (MCs). Western blot and immunofluorescence experiments showed HNF4α expression in MCs. Knockdown of HNF4α using a small interfering RNA approach significantly increased mRNA expression levels of both STIM1 and Orai1 and protein expression levels of STIM1 in cultured human MCs. Consistently, overexpression of HNF4α reduced expressed STIM1 protein expression in human embryonic kidney-293 cells. Furthermore, high glucose treatment did not significantly change the abundance of HNF4α protein in MCs but significantly attenuated HNF4α binding activity to the Stim1 promoter. Moreover, knockdown of HNF4α significantly augmented store-operated Ca2+ entry, which is known to be gated by STIM1 and has recently been found to be antifibrotic in MCs. In agreement with those results, knockdown of HNF4[1] significantly attenuated the fibrotic response of high glucose. These results suggest that HNF4[1] negatively regulates STIM1 transcription in MCs. High glucose increases STIM1 expression levels by impairing HNF4α binding activity to the Stim1 promoter, which subsequently releases Stim1 transcription from HNF4α repression. Since the STIM1-gated storeoperated Ca2+ entry pathway in MCs has an antifibrotic effect, inhibition of HNF4α in MCs might be a potential therapeutic option for diabetic kidney disease.",
keywords = "Diabetic nephropathy, Hepatic nuclear factor 4α, High glucose, Mesangial cells, Storeoperated Ca entry, Stromal interacting molecule 1",
author = "Yanxia Wang and Sarika Chaudhari and Yuezhong Ren and Rong Ma",
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Impairment of hepatic nuclear factor-4α binding to the stim1 promoter contributes to high glucose-induced upregulation of stim1 expression in glomerular mesangial cells. / Wang, Yanxia; Chaudhari, Sarika; Ren, Yuezhong; Ma, Rong.

In: American Journal of Physiology - Renal Physiology, Vol. 308, No. 10, 15.05.2015, p. F1135-F1145.

Research output: Contribution to journalArticleResearchpeer-review

TY - JOUR

T1 - Impairment of hepatic nuclear factor-4α binding to the stim1 promoter contributes to high glucose-induced upregulation of stim1 expression in glomerular mesangial cells

AU - Wang, Yanxia

AU - Chaudhari, Sarika

AU - Ren, Yuezhong

AU - Ma, Rong

PY - 2015/5/15

Y1 - 2015/5/15

N2 - The present study was carried out to investigate if hepatic nuclear factor (HNF)4α contributed to the high glucose-induced increase in stromal interacting molecule (STIM)1 protein abundance in glomerular mesangial cells (MCs). Western blot and immunofluorescence experiments showed HNF4α expression in MCs. Knockdown of HNF4α using a small interfering RNA approach significantly increased mRNA expression levels of both STIM1 and Orai1 and protein expression levels of STIM1 in cultured human MCs. Consistently, overexpression of HNF4α reduced expressed STIM1 protein expression in human embryonic kidney-293 cells. Furthermore, high glucose treatment did not significantly change the abundance of HNF4α protein in MCs but significantly attenuated HNF4α binding activity to the Stim1 promoter. Moreover, knockdown of HNF4α significantly augmented store-operated Ca2+ entry, which is known to be gated by STIM1 and has recently been found to be antifibrotic in MCs. In agreement with those results, knockdown of HNF4[1] significantly attenuated the fibrotic response of high glucose. These results suggest that HNF4[1] negatively regulates STIM1 transcription in MCs. High glucose increases STIM1 expression levels by impairing HNF4α binding activity to the Stim1 promoter, which subsequently releases Stim1 transcription from HNF4α repression. Since the STIM1-gated storeoperated Ca2+ entry pathway in MCs has an antifibrotic effect, inhibition of HNF4α in MCs might be a potential therapeutic option for diabetic kidney disease.

AB - The present study was carried out to investigate if hepatic nuclear factor (HNF)4α contributed to the high glucose-induced increase in stromal interacting molecule (STIM)1 protein abundance in glomerular mesangial cells (MCs). Western blot and immunofluorescence experiments showed HNF4α expression in MCs. Knockdown of HNF4α using a small interfering RNA approach significantly increased mRNA expression levels of both STIM1 and Orai1 and protein expression levels of STIM1 in cultured human MCs. Consistently, overexpression of HNF4α reduced expressed STIM1 protein expression in human embryonic kidney-293 cells. Furthermore, high glucose treatment did not significantly change the abundance of HNF4α protein in MCs but significantly attenuated HNF4α binding activity to the Stim1 promoter. Moreover, knockdown of HNF4α significantly augmented store-operated Ca2+ entry, which is known to be gated by STIM1 and has recently been found to be antifibrotic in MCs. In agreement with those results, knockdown of HNF4[1] significantly attenuated the fibrotic response of high glucose. These results suggest that HNF4[1] negatively regulates STIM1 transcription in MCs. High glucose increases STIM1 expression levels by impairing HNF4α binding activity to the Stim1 promoter, which subsequently releases Stim1 transcription from HNF4α repression. Since the STIM1-gated storeoperated Ca2+ entry pathway in MCs has an antifibrotic effect, inhibition of HNF4α in MCs might be a potential therapeutic option for diabetic kidney disease.

KW - Diabetic nephropathy

KW - Hepatic nuclear factor 4α

KW - High glucose

KW - Mesangial cells

KW - Storeoperated Ca entry

KW - Stromal interacting molecule 1

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U2 - 10.1152/ajprenal.00563.2014

DO - 10.1152/ajprenal.00563.2014

M3 - Article

VL - 308

SP - F1135-F1145

JO - American Journal of Physiology - Renal Physiology

JF - American Journal of Physiology - Renal Physiology

SN - 0363-6127

IS - 10

ER -