Impact of substrate overloading on archaeal populations in anaerobic digestion of animal waste

S. Chen, E. M. Zamudio Cañas, Yan Zhang, Z. Zhu, Q. He

Research output: Contribution to journalArticleResearchpeer-review

31 Citations (Scopus)

Abstract

Aims: To characterize adaptive changes in methanogenic microbial community in response to substrate overloading and identify potential linkages between process performance and microbial community composition. Methods and Results: Triplicate continuous anaerobic digesters were developed as model anaerobic digestion processes, which were subsequently disrupted by substrate overloading. The clone library analysis of archaeal communities experiencing substrate overloading showed that populations related to Methanosaeta were the dominant methanogens before and after substrate overloading, suggesting the functional importance of these acetoclastic methanogens in balanced anaerobic digestion processes characterized with low organic acids concentrations. Population redundancy in Methanosaeta increased following substrate overloading with the emergence of additional populations of Methanosaeta. More importantly, the methanogenic community responded to process imbalance with greater functional diversity with increased abundance of functionally distinct hydrogenotrophic and acetoclastic methanogens, which likely enhanced the functional stability of anaerobic digestion during disruptions in the anaerobic food web under process perturbation. Crenarchaeota were identified as persistent constituents of the archaeal communities in anaerobic digestion, warranting further efforts to identifying the functions of these phylogenetically distinct populations in anaerobic digestion. Conclusions: Substrate overloading in anaerobic digestion resulted in an increased functional diversity of the methanogenic community, which enhanced the capacity to overcome subsequent occurrences of process perturbations without performance disruption, providing a potential strategy to maintain process stability in anaerobic digestion. Significance and Impact of the Study: Anaerobic digestion is a sustainable option for waste treatment and renewable energy production. However, process instability resulting from variations in substrate loading has been one of the obstacles to the broader adoption of anaerobic digestion technology. Insight into the linkages between process performance and microbial community gained in this study is valuable for developing strategies for the mitigation of the impact of substrate overloading on anaerobic digestion processes.

Original languageEnglish
Pages (from-to)1371-1379
Number of pages9
JournalJournal of Applied Microbiology
Volume113
Issue number6
DOIs
StatePublished - 1 Dec 2012

Fingerprint

Digestion
Population
Crenarchaeota
Renewable Energy
Food Chain
Clone Cells
Technology
Acids

Keywords

  • Archaea
  • Biogas
  • Crenarchaeota
  • Methane
  • Microbial community
  • Perturbation

Cite this

Chen, S. ; Zamudio Cañas, E. M. ; Zhang, Yan ; Zhu, Z. ; He, Q. / Impact of substrate overloading on archaeal populations in anaerobic digestion of animal waste. In: Journal of Applied Microbiology. 2012 ; Vol. 113, No. 6. pp. 1371-1379.
@article{87d014cc1f0148ee9589f44094e4c5fa,
title = "Impact of substrate overloading on archaeal populations in anaerobic digestion of animal waste",
abstract = "Aims: To characterize adaptive changes in methanogenic microbial community in response to substrate overloading and identify potential linkages between process performance and microbial community composition. Methods and Results: Triplicate continuous anaerobic digesters were developed as model anaerobic digestion processes, which were subsequently disrupted by substrate overloading. The clone library analysis of archaeal communities experiencing substrate overloading showed that populations related to Methanosaeta were the dominant methanogens before and after substrate overloading, suggesting the functional importance of these acetoclastic methanogens in balanced anaerobic digestion processes characterized with low organic acids concentrations. Population redundancy in Methanosaeta increased following substrate overloading with the emergence of additional populations of Methanosaeta. More importantly, the methanogenic community responded to process imbalance with greater functional diversity with increased abundance of functionally distinct hydrogenotrophic and acetoclastic methanogens, which likely enhanced the functional stability of anaerobic digestion during disruptions in the anaerobic food web under process perturbation. Crenarchaeota were identified as persistent constituents of the archaeal communities in anaerobic digestion, warranting further efforts to identifying the functions of these phylogenetically distinct populations in anaerobic digestion. Conclusions: Substrate overloading in anaerobic digestion resulted in an increased functional diversity of the methanogenic community, which enhanced the capacity to overcome subsequent occurrences of process perturbations without performance disruption, providing a potential strategy to maintain process stability in anaerobic digestion. Significance and Impact of the Study: Anaerobic digestion is a sustainable option for waste treatment and renewable energy production. However, process instability resulting from variations in substrate loading has been one of the obstacles to the broader adoption of anaerobic digestion technology. Insight into the linkages between process performance and microbial community gained in this study is valuable for developing strategies for the mitigation of the impact of substrate overloading on anaerobic digestion processes.",
keywords = "Archaea, Biogas, Crenarchaeota, Methane, Microbial community, Perturbation",
author = "S. Chen and {Zamudio Ca{\~n}as}, {E. M.} and Yan Zhang and Z. Zhu and Q. He",
year = "2012",
month = "12",
day = "1",
doi = "10.1111/jam.12001",
language = "English",
volume = "113",
pages = "1371--1379",
journal = "Journal of Applied Microbiology",
issn = "1364-5072",
publisher = "Wiley-Blackwell Publishing Ltd",
number = "6",

}

Impact of substrate overloading on archaeal populations in anaerobic digestion of animal waste. / Chen, S.; Zamudio Cañas, E. M.; Zhang, Yan; Zhu, Z.; He, Q.

In: Journal of Applied Microbiology, Vol. 113, No. 6, 01.12.2012, p. 1371-1379.

Research output: Contribution to journalArticleResearchpeer-review

TY - JOUR

T1 - Impact of substrate overloading on archaeal populations in anaerobic digestion of animal waste

AU - Chen, S.

AU - Zamudio Cañas, E. M.

AU - Zhang, Yan

AU - Zhu, Z.

AU - He, Q.

PY - 2012/12/1

Y1 - 2012/12/1

N2 - Aims: To characterize adaptive changes in methanogenic microbial community in response to substrate overloading and identify potential linkages between process performance and microbial community composition. Methods and Results: Triplicate continuous anaerobic digesters were developed as model anaerobic digestion processes, which were subsequently disrupted by substrate overloading. The clone library analysis of archaeal communities experiencing substrate overloading showed that populations related to Methanosaeta were the dominant methanogens before and after substrate overloading, suggesting the functional importance of these acetoclastic methanogens in balanced anaerobic digestion processes characterized with low organic acids concentrations. Population redundancy in Methanosaeta increased following substrate overloading with the emergence of additional populations of Methanosaeta. More importantly, the methanogenic community responded to process imbalance with greater functional diversity with increased abundance of functionally distinct hydrogenotrophic and acetoclastic methanogens, which likely enhanced the functional stability of anaerobic digestion during disruptions in the anaerobic food web under process perturbation. Crenarchaeota were identified as persistent constituents of the archaeal communities in anaerobic digestion, warranting further efforts to identifying the functions of these phylogenetically distinct populations in anaerobic digestion. Conclusions: Substrate overloading in anaerobic digestion resulted in an increased functional diversity of the methanogenic community, which enhanced the capacity to overcome subsequent occurrences of process perturbations without performance disruption, providing a potential strategy to maintain process stability in anaerobic digestion. Significance and Impact of the Study: Anaerobic digestion is a sustainable option for waste treatment and renewable energy production. However, process instability resulting from variations in substrate loading has been one of the obstacles to the broader adoption of anaerobic digestion technology. Insight into the linkages between process performance and microbial community gained in this study is valuable for developing strategies for the mitigation of the impact of substrate overloading on anaerobic digestion processes.

AB - Aims: To characterize adaptive changes in methanogenic microbial community in response to substrate overloading and identify potential linkages between process performance and microbial community composition. Methods and Results: Triplicate continuous anaerobic digesters were developed as model anaerobic digestion processes, which were subsequently disrupted by substrate overloading. The clone library analysis of archaeal communities experiencing substrate overloading showed that populations related to Methanosaeta were the dominant methanogens before and after substrate overloading, suggesting the functional importance of these acetoclastic methanogens in balanced anaerobic digestion processes characterized with low organic acids concentrations. Population redundancy in Methanosaeta increased following substrate overloading with the emergence of additional populations of Methanosaeta. More importantly, the methanogenic community responded to process imbalance with greater functional diversity with increased abundance of functionally distinct hydrogenotrophic and acetoclastic methanogens, which likely enhanced the functional stability of anaerobic digestion during disruptions in the anaerobic food web under process perturbation. Crenarchaeota were identified as persistent constituents of the archaeal communities in anaerobic digestion, warranting further efforts to identifying the functions of these phylogenetically distinct populations in anaerobic digestion. Conclusions: Substrate overloading in anaerobic digestion resulted in an increased functional diversity of the methanogenic community, which enhanced the capacity to overcome subsequent occurrences of process perturbations without performance disruption, providing a potential strategy to maintain process stability in anaerobic digestion. Significance and Impact of the Study: Anaerobic digestion is a sustainable option for waste treatment and renewable energy production. However, process instability resulting from variations in substrate loading has been one of the obstacles to the broader adoption of anaerobic digestion technology. Insight into the linkages between process performance and microbial community gained in this study is valuable for developing strategies for the mitigation of the impact of substrate overloading on anaerobic digestion processes.

KW - Archaea

KW - Biogas

KW - Crenarchaeota

KW - Methane

KW - Microbial community

KW - Perturbation

UR - http://www.scopus.com/inward/record.url?scp=84869866130&partnerID=8YFLogxK

U2 - 10.1111/jam.12001

DO - 10.1111/jam.12001

M3 - Article

VL - 113

SP - 1371

EP - 1379

JO - Journal of Applied Microbiology

JF - Journal of Applied Microbiology

SN - 1364-5072

IS - 6

ER -