Imaging viscosity of intragranular mucin matrix in cystic fibrosis cells

Sebastian Requena, Olga Ponomarchuk, Marlius Castillo, Jonathan Rebik, Emmanuelle Brochiero, Julian Borejdo, Ignacy Gryczynski, Sergei V. Dzyuba, Zygmunt Gryczynski, Ryszard Grygorczyk, Rafal Fudala

Research output: Contribution to journalArticle

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Abstract

Abnormalities of mucus viscosity play a critical role in the pathogenesis of several respiratory diseases, including cystic fibrosis. Currently, there are no approaches to assess the rheological properties of mucin granule matrices in live cells. This is the first example of the use of a molecular rotor, a BODIPY dye, to quantitatively visualize the viscosity of intragranular mucin matrices in a large population of individual granules in differentiated primary bronchial epithelial cells using fluorescence lifetime imaging microscopy.

Original languageEnglish
Article number16761
JournalScientific Reports
Volume7
Issue number1
DOIs
StatePublished - 1 Dec 2017

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