@article{6e4d048f07bc4596b927c7a80261fdc9,
title = "Imaging viscosity of intragranular mucin matrix in cystic fibrosis cells",
abstract = "Abnormalities of mucus viscosity play a critical role in the pathogenesis of several respiratory diseases, including cystic fibrosis. Currently, there are no approaches to assess the rheological properties of mucin granule matrices in live cells. This is the first example of the use of a molecular rotor, a BODIPY dye, to quantitatively visualize the viscosity of intragranular mucin matrices in a large population of individual granules in differentiated primary bronchial epithelial cells using fluorescence lifetime imaging microscopy.",
author = "Sebastian Requena and Olga Ponomarchuk and Marlius Castillo and Jonathan Rebik and Emmanuelle Brochiero and Julian Borejdo and Ignacy Gryczynski and Dzyuba, {Sergei V.} and Zygmunt Gryczynski and Ryszard Grygorczyk and Rafal Fudala",
note = "Funding Information: This work was supported by research grants from the Cystic Fibrosis Canada (R.G.), Canadian Institute of Health Research, PJT148593 (E.B.), NSF grant CBET-1264608 (I.G.), and the Respiratory Health Network of Fonds de Recherche du Qu{\'e}bec – Sant{\'e} (FRQS) (Respiratory Tissue Biobank of CRCHUM). The authors would like to thank Primary Airway Cell Biobank (PACB) of Cystic Fibrosis Translational Research Center (McGill University). The authors also thank the reviewers for useful suggestions. Publisher Copyright: {\textcopyright} 2017 The Author(s).",
year = "2017",
month = dec,
day = "1",
doi = "10.1038/s41598-017-17037-2",
language = "English",
volume = "7",
journal = "Scientific Reports",
issn = "2045-2322",
publisher = "Nature Publishing Group",
number = "1",
}