Imaging viscosity of intragranular mucin matrix in cystic fibrosis cells

Sebastian Requena, Olga Ponomarchuk, Marlius Castillo, Jonathan Rebik, Emmanuelle Brochiero, Julian Borejdo, Ignacy Gryczynski, Sergei V. Dzyuba, Zygmunt Gryczynski, Ryszard Grygorczyk, Rafal Fudala

Research output: Contribution to journalArticleResearchpeer-review

2 Citations (Scopus)

Abstract

Abnormalities of mucus viscosity play a critical role in the pathogenesis of several respiratory diseases, including cystic fibrosis. Currently, there are no approaches to assess the rheological properties of mucin granule matrices in live cells. This is the first example of the use of a molecular rotor, a BODIPY dye, to quantitatively visualize the viscosity of intragranular mucin matrices in a large population of individual granules in differentiated primary bronchial epithelial cells using fluorescence lifetime imaging microscopy.

Original languageEnglish
Article number16761
JournalScientific Reports
Volume7
Issue number1
DOIs
StatePublished - 1 Dec 2017

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Mucins
Cystic Fibrosis
Viscosity
Optical Imaging
Mucus
Microscopy
Coloring Agents
Epithelial Cells
Population
4,4-difluoro-4-bora-3a,4a-diaza-s-indacene

Cite this

Requena, Sebastian ; Ponomarchuk, Olga ; Castillo, Marlius ; Rebik, Jonathan ; Brochiero, Emmanuelle ; Borejdo, Julian ; Gryczynski, Ignacy ; Dzyuba, Sergei V. ; Gryczynski, Zygmunt ; Grygorczyk, Ryszard ; Fudala, Rafal. / Imaging viscosity of intragranular mucin matrix in cystic fibrosis cells. In: Scientific Reports. 2017 ; Vol. 7, No. 1.
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abstract = "Abnormalities of mucus viscosity play a critical role in the pathogenesis of several respiratory diseases, including cystic fibrosis. Currently, there are no approaches to assess the rheological properties of mucin granule matrices in live cells. This is the first example of the use of a molecular rotor, a BODIPY dye, to quantitatively visualize the viscosity of intragranular mucin matrices in a large population of individual granules in differentiated primary bronchial epithelial cells using fluorescence lifetime imaging microscopy.",
author = "Sebastian Requena and Olga Ponomarchuk and Marlius Castillo and Jonathan Rebik and Emmanuelle Brochiero and Julian Borejdo and Ignacy Gryczynski and Dzyuba, {Sergei V.} and Zygmunt Gryczynski and Ryszard Grygorczyk and Rafal Fudala",
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Requena, S, Ponomarchuk, O, Castillo, M, Rebik, J, Brochiero, E, Borejdo, J, Gryczynski, I, Dzyuba, SV, Gryczynski, Z, Grygorczyk, R & Fudala, R 2017, 'Imaging viscosity of intragranular mucin matrix in cystic fibrosis cells', Scientific Reports, vol. 7, no. 1, 16761. https://doi.org/10.1038/s41598-017-17037-2

Imaging viscosity of intragranular mucin matrix in cystic fibrosis cells. / Requena, Sebastian; Ponomarchuk, Olga; Castillo, Marlius; Rebik, Jonathan; Brochiero, Emmanuelle; Borejdo, Julian; Gryczynski, Ignacy; Dzyuba, Sergei V.; Gryczynski, Zygmunt; Grygorczyk, Ryszard; Fudala, Rafal.

In: Scientific Reports, Vol. 7, No. 1, 16761, 01.12.2017.

Research output: Contribution to journalArticleResearchpeer-review

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AU - Requena, Sebastian

AU - Ponomarchuk, Olga

AU - Castillo, Marlius

AU - Rebik, Jonathan

AU - Brochiero, Emmanuelle

AU - Borejdo, Julian

AU - Gryczynski, Ignacy

AU - Dzyuba, Sergei V.

AU - Gryczynski, Zygmunt

AU - Grygorczyk, Ryszard

AU - Fudala, Rafal

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