Identification of West Eurasian mitochondrial haplogroups by mtDNA SNP screening: Results of the 2006-2007 EDNAP collaborative exercise

Walther Parson; Liane Fendt; David Ballard; Claus Børsting; Bernd Brinkmann; Ángel Carracedo; Mónica Carvalho; Michael D. Coble; Francisco Corte Real; Stijn Desmyter; Berit M. Dupuy; Cheryl Harrison; Carsten Hohoff; Rebecca Just; Tanja Krämer; Niels Morling; Antonio Salas; Hermann Schmitter; Peter M. Schneider; Marie Luise Sonntag; Peter M. Vallone; Anita Brandstätter

doi:10.1016/j.fsigen.2007.08.007

Identification of West Eurasian mitochondrial haplogroups by mtDNA SNP screening: Results of the 2006-2007 EDNAP collaborative exercise

Walther Parson, Liane Fendt, David Ballard, Claus Børsting, Bernd Brinkmann, Ángel Carracedo, Mónica Carvalho, Michael D. Coble, Francisco Corte Real, Stijn Desmyter, Berit M. Dupuy, Cheryl Harrison, Carsten Hohoff, Rebecca Just, Tanja Krämer, Niels Morling, Antonio Salas, Hermann Schmitter, Peter M. Schneider, Marie Luise SonntagPeter M. Vallone, Anita Brandstätter

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16 Scopus citations

Abstract

The European DNA Profiling (EDNAP) Group performed a collaborative exercise on a mitochondrial (mt) DNA screening assay that targeted 16 nucleotide positions in the coding region and allowed for the discrimination of major west Eurasian mtDNA haplogroups. The purpose of the exercise was to evaluate the stability and reproducibility of the self-developed multiplex-PCR and multiplex-single base extension kit by blind-testing saliva and hair shaft samples provided by the organizing laboratory. The overall success rate in obtaining useful results was high given that some of the participating laboratories had no previous experience with the technology and/or mtDNA analysis. The results of this collaborative exercise stimulate the expansion of screening methods in forensic laboratories to increase efficiency and performance of mtDNA typing, and thus demonstrates that mtDNA SNP typing is a powerful tool for forensic casework analysis.

Original language	English
Pages (from-to)	61-68
Number of pages	8
Journal	Forensic Science International: Genetics
Volume	2
Issue number	1
DOIs	https://doi.org/10.1016/j.fsigen.2007.08.007
State	Published - Jan 2008

Keywords

Coding region SNPs
Forensic identification
Mitochondrial DNA
Multiplex
SNaPshot

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Parson, W., Fendt, L., Ballard, D., Børsting, C., Brinkmann, B., Carracedo, Á., Carvalho, M., Coble, M. D., Real, F. C., Desmyter, S., Dupuy, B. M., Harrison, C., Hohoff, C., Just, R., Krämer, T., Morling, N., Salas, A., Schmitter, H., Schneider, P. M., ... Brandstätter, A. (2008). Identification of West Eurasian mitochondrial haplogroups by mtDNA SNP screening: Results of the 2006-2007 EDNAP collaborative exercise. Forensic Science International: Genetics, 2(1), 61-68. https://doi.org/10.1016/j.fsigen.2007.08.007

@article{b88dacfec87b4ecd944ca4030fbe44f6,

title = "Identification of West Eurasian mitochondrial haplogroups by mtDNA SNP screening: Results of the 2006-2007 EDNAP collaborative exercise",

abstract = "The European DNA Profiling (EDNAP) Group performed a collaborative exercise on a mitochondrial (mt) DNA screening assay that targeted 16 nucleotide positions in the coding region and allowed for the discrimination of major west Eurasian mtDNA haplogroups. The purpose of the exercise was to evaluate the stability and reproducibility of the self-developed multiplex-PCR and multiplex-single base extension kit by blind-testing saliva and hair shaft samples provided by the organizing laboratory. The overall success rate in obtaining useful results was high given that some of the participating laboratories had no previous experience with the technology and/or mtDNA analysis. The results of this collaborative exercise stimulate the expansion of screening methods in forensic laboratories to increase efficiency and performance of mtDNA typing, and thus demonstrates that mtDNA SNP typing is a powerful tool for forensic casework analysis.",

keywords = "Coding region SNPs, Forensic identification, Mitochondrial DNA, Multiplex, SNaPshot",

author = "Walther Parson and Liane Fendt and David Ballard and Claus B{\o}rsting and Bernd Brinkmann and {\'A}ngel Carracedo and M{\'o}nica Carvalho and Coble, {Michael D.} and Real, {Francisco Corte} and Stijn Desmyter and Dupuy, {Berit M.} and Cheryl Harrison and Carsten Hohoff and Rebecca Just and Tanja Kr{\"a}mer and Niels Morling and Antonio Salas and Hermann Schmitter and Schneider, {Peter M.} and Sonntag, {Marie Luise} and Vallone, {Peter M.} and Anita Brandst{\"a}tter",

year = "2008",

month = jan,

doi = "10.1016/j.fsigen.2007.08.007",

language = "English",

volume = "2",

pages = "61--68",

journal = "Forensic Science International: Genetics",

issn = "1872-4973",

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}

Parson, W, Fendt, L, Ballard, D, Børsting, C, Brinkmann, B, Carracedo, Á, Carvalho, M, Coble, MD, Real, FC, Desmyter, S, Dupuy, BM, Harrison, C, Hohoff, C, Just, R, Krämer, T, Morling, N, Salas, A, Schmitter, H, Schneider, PM, Sonntag, ML, Vallone, PM & Brandstätter, A 2008, 'Identification of West Eurasian mitochondrial haplogroups by mtDNA SNP screening: Results of the 2006-2007 EDNAP collaborative exercise', Forensic Science International: Genetics, vol. 2, no. 1, pp. 61-68. https://doi.org/10.1016/j.fsigen.2007.08.007

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T1 - Identification of West Eurasian mitochondrial haplogroups by mtDNA SNP screening

T2 - Results of the 2006-2007 EDNAP collaborative exercise

AU - Parson, Walther

AU - Fendt, Liane

AU - Ballard, David

AU - Børsting, Claus

AU - Brinkmann, Bernd

AU - Carracedo, Ángel

AU - Carvalho, Mónica

AU - Coble, Michael D.

AU - Real, Francisco Corte

AU - Desmyter, Stijn

AU - Dupuy, Berit M.

AU - Harrison, Cheryl

AU - Hohoff, Carsten

AU - Just, Rebecca

AU - Krämer, Tanja

AU - Morling, Niels

AU - Salas, Antonio

AU - Schmitter, Hermann

AU - Schneider, Peter M.

AU - Sonntag, Marie Luise

AU - Vallone, Peter M.

AU - Brandstätter, Anita

PY - 2008/1

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AB - The European DNA Profiling (EDNAP) Group performed a collaborative exercise on a mitochondrial (mt) DNA screening assay that targeted 16 nucleotide positions in the coding region and allowed for the discrimination of major west Eurasian mtDNA haplogroups. The purpose of the exercise was to evaluate the stability and reproducibility of the self-developed multiplex-PCR and multiplex-single base extension kit by blind-testing saliva and hair shaft samples provided by the organizing laboratory. The overall success rate in obtaining useful results was high given that some of the participating laboratories had no previous experience with the technology and/or mtDNA analysis. The results of this collaborative exercise stimulate the expansion of screening methods in forensic laboratories to increase efficiency and performance of mtDNA typing, and thus demonstrates that mtDNA SNP typing is a powerful tool for forensic casework analysis.

KW - Coding region SNPs

KW - Forensic identification

KW - Mitochondrial DNA

KW - Multiplex

KW - SNaPshot

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JO - Forensic Science International: Genetics

JF - Forensic Science International: Genetics

IS - 1

ER -

Identification of West Eurasian mitochondrial haplogroups by mtDNA SNP screening: Results of the 2006-2007 EDNAP collaborative exercise

Abstract

Keywords

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