Identification of West Eurasian mitochondrial haplogroups by mtDNA SNP screening: Results of the 2006-2007 EDNAP collaborative exercise

Walther Parson, Liane Fendt, David Ballard, Claus Børsting, Bernd Brinkmann, Ángel Carracedo, Mónica Carvalho, Michael Dewitt Coble, Francisco Corte Real, Stijn Desmyter, Berit M. Dupuy, Cheryl Harrison, Carsten Hohoff, Rebecca Just, Tanja Krämer, Niels Morling, Antonio Salas, Hermann Schmitter, Peter M. Schneider, Marie Luise SonntagPeter M. Vallone, Anita Brandstätter

Research output: Contribution to journalArticle

14 Scopus citations

Abstract

The European DNA Profiling (EDNAP) Group performed a collaborative exercise on a mitochondrial (mt) DNA screening assay that targeted 16 nucleotide positions in the coding region and allowed for the discrimination of major west Eurasian mtDNA haplogroups. The purpose of the exercise was to evaluate the stability and reproducibility of the self-developed multiplex-PCR and multiplex-single base extension kit by blind-testing saliva and hair shaft samples provided by the organizing laboratory. The overall success rate in obtaining useful results was high given that some of the participating laboratories had no previous experience with the technology and/or mtDNA analysis. The results of this collaborative exercise stimulate the expansion of screening methods in forensic laboratories to increase efficiency and performance of mtDNA typing, and thus demonstrates that mtDNA SNP typing is a powerful tool for forensic casework analysis.

Original languageEnglish
Pages (from-to)61-68
Number of pages8
JournalForensic Science International: Genetics
Volume2
Issue number1
DOIs
StatePublished - 1 Jan 2008

Keywords

  • Coding region SNPs
  • Forensic identification
  • Mitochondrial DNA
  • Multiplex
  • SNaPshot

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    Parson, W., Fendt, L., Ballard, D., Børsting, C., Brinkmann, B., Carracedo, Á., Carvalho, M., Coble, M. D., Real, F. C., Desmyter, S., Dupuy, B. M., Harrison, C., Hohoff, C., Just, R., Krämer, T., Morling, N., Salas, A., Schmitter, H., Schneider, P. M., ... Brandstätter, A. (2008). Identification of West Eurasian mitochondrial haplogroups by mtDNA SNP screening: Results of the 2006-2007 EDNAP collaborative exercise. Forensic Science International: Genetics, 2(1), 61-68. https://doi.org/10.1016/j.fsigen.2007.08.007