TY - JOUR
T1 - Identification of trans-acting factors that interact with cis-acting elements present in the first nontranslated exon of the human apolipoprotein B gene
AU - Chuang, Samuel S.
AU - Das, Hriday K.
PY - 1996/3/27
Y1 - 1996/3/27
N2 - Apolipoprotein B is the sole protein of low density lipoprotein and is produced primarily in the liver. Previously, we have identified two cis-acting elements (+20 to +40; +43 to +53) in the non-translated exon of the human apolipoprotein B gene, using DNase I footprint analysis. Wild type and mutated promoter constructs were used as templates in DNase I footprint analysis with rat liver nuclear extracts. These experiments suggest that trans-acting factors BRF-3 and BRF-4 which recognize these two footprint regions (+20 to +40; +43 to +53) respectively, act independently. In vitro-synthesized hepatocyte nuclear factors HNF-1α, HNF-1β, HNF-3α and HNF-2/HNF-4 showed no specific protein/DNA interaction with these regions. DNase I footprint analysis using other DNA-binding site oligonucleotides as competitors indicated that BRF-3 and BRF-4 could be different hepatocyte nuclear factors and may contribute to the regulation of transcription of the human apolipoprotein B gene.
AB - Apolipoprotein B is the sole protein of low density lipoprotein and is produced primarily in the liver. Previously, we have identified two cis-acting elements (+20 to +40; +43 to +53) in the non-translated exon of the human apolipoprotein B gene, using DNase I footprint analysis. Wild type and mutated promoter constructs were used as templates in DNase I footprint analysis with rat liver nuclear extracts. These experiments suggest that trans-acting factors BRF-3 and BRF-4 which recognize these two footprint regions (+20 to +40; +43 to +53) respectively, act independently. In vitro-synthesized hepatocyte nuclear factors HNF-1α, HNF-1β, HNF-3α and HNF-2/HNF-4 showed no specific protein/DNA interaction with these regions. DNase I footprint analysis using other DNA-binding site oligonucleotides as competitors indicated that BRF-3 and BRF-4 could be different hepatocyte nuclear factors and may contribute to the regulation of transcription of the human apolipoprotein B gene.
UR - http://www.scopus.com/inward/record.url?scp=0029995653&partnerID=8YFLogxK
U2 - 10.1006/bbrc.1996.0442
DO - 10.1006/bbrc.1996.0442
M3 - Article
C2 - 8607803
AN - SCOPUS:0029995653
SN - 0006-291X
VL - 220
SP - 553
EP - 562
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
IS - 3
ER -