The identification of synaptosomal plasma membrane (SPM) proteins by 2D-separation techniques combined with mass spectrometry (MS) was described. The synaptosomes were isolated from rat brain by using continuous sucrose density gradients. The SPM proteins were separated by 1D SDS-PAGE and selected bands digested in-gel with sequencing-grade modified trypsin obtained from Promega. Digested synaptosomal plasma membrane proteins were purified with ZipTip microcolumns and analyzed by a Voyager DE-Pro MALDI-TOF mass spectrometer (MS) operated in reflector mode.
|Number of pages||2|
|State||Published - 1 Dec 2002|
|Event||Proceedings - 50th ASMS Conference on Mass Spectrometry and Allied Topics - Orlando, FL, United States|
Duration: 2 Jun 2002 → 6 Jun 2002
|Other||Proceedings - 50th ASMS Conference on Mass Spectrometry and Allied Topics|
|Period||2/06/02 → 6/06/02|