Identification of synaptosomal plasma membrane proteins by 2D-separation techniques combined with mass spectrometry

Stanley M. Stevens, Laszlo Prokai

Research output: Contribution to conferencePaperpeer-review

Abstract

The identification of synaptosomal plasma membrane (SPM) proteins by 2D-separation techniques combined with mass spectrometry (MS) was described. The synaptosomes were isolated from rat brain by using continuous sucrose density gradients. The SPM proteins were separated by 1D SDS-PAGE and selected bands digested in-gel with sequencing-grade modified trypsin obtained from Promega. Digested synaptosomal plasma membrane proteins were purified with ZipTip microcolumns and analyzed by a Voyager DE-Pro MALDI-TOF mass spectrometer (MS) operated in reflector mode.

Original languageEnglish
Pages245-246
Number of pages2
StatePublished - 1 Dec 2002
EventProceedings - 50th ASMS Conference on Mass Spectrometry and Allied Topics - Orlando, FL, United States
Duration: 2 Jun 20026 Jun 2002

Other

OtherProceedings - 50th ASMS Conference on Mass Spectrometry and Allied Topics
Country/TerritoryUnited States
CityOrlando, FL
Period2/06/026/06/02

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