Abstract
The identification of synaptosomal plasma membrane (SPM) proteins by 2D-separation techniques combined with mass spectrometry (MS) was described. The synaptosomes were isolated from rat brain by using continuous sucrose density gradients. The SPM proteins were separated by 1D SDS-PAGE and selected bands digested in-gel with sequencing-grade modified trypsin obtained from Promega. Digested synaptosomal plasma membrane proteins were purified with ZipTip microcolumns and analyzed by a Voyager DE-Pro MALDI-TOF mass spectrometer (MS) operated in reflector mode.
| Original language | English |
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| Pages | 245-246 |
| Number of pages | 2 |
| State | Published - 1 Dec 2002 |
| Event | Proceedings - 50th ASMS Conference on Mass Spectrometry and Allied Topics - Orlando, FL, United States Duration: 2 Jun 2002 → 6 Jun 2002 |
Other
| Other | Proceedings - 50th ASMS Conference on Mass Spectrometry and Allied Topics |
|---|---|
| Country/Territory | United States |
| City | Orlando, FL |
| Period | 2/06/02 → 6/06/02 |