Abstract
During an extensive multipopulation study with Y-short tandem repeat (STR) loci, amplified using the AmpFℓSTR® Yfiler™ PCR amplification kit, amplification of a 71 bp fragment was observed in 2.32% of the male samples analyzed (N=3141). By direct sequencing of this fragment, it was determined that the primer binding sequences were identical to those of the DYS456 locus. A T to G single-nucleotide polymorphism (SNP) enabled amplification of the 71 bp fragment. The SNP is located within an X-Y homologous region at Xq21.31 and was observed with the highest frequency within the African American and Sub-Saharan African populations in our study. Presence of SNP on the X chromosome did not interfere with the reliability of typing the DYS456 locus and the other Y-STR loci typeable using the AmpFℓSTR® Yfiler™ PCR amplification kit. Full profiles in a mixture of male:female at 1:4000 were obtained using the current configuration of the AmpFℓSTR Yfiler kit even in the presence of female DNA containing the G variant.
Original language | English |
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Pages (from-to) | 344-348 |
Number of pages | 5 |
Journal | Journal of Forensic Sciences |
Volume | 51 |
Issue number | 2 |
DOIs | |
State | Published - 1 Mar 2006 |
Keywords
- DNA typing
- DYS456
- Forensic science
- Homolog
- Polymerase chain reaction (PCR)
- Short tandem repeat (STR)
- Single-nucleotide polymorphism (SNP)
- Y chromosome
- Y-STR
- Yfiler