TY - JOUR
T1 - Identification of a leukemia-associated antigen of human acute lymphocytic leukemia
AU - Veit, Bruce C.
AU - Melvin, Susan L.
AU - Bowman, W. Paul
N1 - Funding Information:
I Received August 21, 1979; accepted December 2, 1979. 2 Supported by grant IN-99E from the American Cancer Society; by Public Health Service grant RR05584 from the Division of Research Resources, National Institutes of Health; and by American Lebanese-Syrian Associated Charities. J Research procedures were in accord with the ethical standards of the Clinical Trials Committee, St. Jude Children's Research Hospital. 4 Division of Immunology, St. Jude Children's Research Hospital, 332 North Lauderdale, P.O. Box 318, Memphis, Tenn. 38101.
PY - 1980/6
Y1 - 1980/6
N2 - A human leukemia-associated antigen (LAA) has been Identified by Immunofluorescence and electrophoretic analyses. LAA was detected on the surfaces of cells from patients with acute lymphocytic leukemia (ALL) as well as on the surfaces of leukemia cells from the established cell lines NALM-1, NALM- 16, MOLT-4, CCRF-CEM, and RPMI 8402. The antigen was not detected on BALM-1 or Raji cells (established B-cell lines), bone marrow cells from ALL patients In remission, or on blood lymphocytes from normal donors. This antigen was most frequently associated with common ALL (cALL); however, cells from 2 of 12 patients with T-cell ALL and 1 patient with B-cell ALL also expressed this antigen. Under reduced conditions, the antigen had an approximate molecular mass of 100, 000 daltons as determined by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and autoradiographic analysis and appeared to be the same cALL antigen that has recently been described by others. The probability that LAA Is a normal differentiation antigen was discussed.
AB - A human leukemia-associated antigen (LAA) has been Identified by Immunofluorescence and electrophoretic analyses. LAA was detected on the surfaces of cells from patients with acute lymphocytic leukemia (ALL) as well as on the surfaces of leukemia cells from the established cell lines NALM-1, NALM- 16, MOLT-4, CCRF-CEM, and RPMI 8402. The antigen was not detected on BALM-1 or Raji cells (established B-cell lines), bone marrow cells from ALL patients In remission, or on blood lymphocytes from normal donors. This antigen was most frequently associated with common ALL (cALL); however, cells from 2 of 12 patients with T-cell ALL and 1 patient with B-cell ALL also expressed this antigen. Under reduced conditions, the antigen had an approximate molecular mass of 100, 000 daltons as determined by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and autoradiographic analysis and appeared to be the same cALL antigen that has recently been described by others. The probability that LAA Is a normal differentiation antigen was discussed.
UR - http://www.scopus.com/inward/record.url?scp=0018821809&partnerID=8YFLogxK
U2 - 10.1093/jnci/64.6.1321
DO - 10.1093/jnci/64.6.1321
M3 - Article
C2 - 6154821
AN - SCOPUS:0018821809
SN - 0027-8874
VL - 64
SP - 1321
EP - 1328
JO - Journal of the National Cancer Institute
JF - Journal of the National Cancer Institute
IS - 6
ER -