Identification of a human LIM-Hox gene, hLH-2, aberrantly expressed in chronic myelogenous leukaemia and located on 9q33-34.1

Hua Kang Wu, Henry H.Q. Heng, David P. Siderovski, Wei Feng Dong, Yoshiaki Okuno, Xiao Mei Shi, Lap Chee Tsui, Mark D. Minden

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Abstract

We describe the isolation of human LH-2, a putative transcription factor containing two cysteine-rich regions (LIM domains) and a homeobox (Hox) DNA-binding domain. High levels of hLH-2 expression were observed in all cases of chronic myelogenous leukaemia (CML) tested, regardless of disease status. hLH-2 was mapped to chromosome 9q33-34.1, in the same region as the reciprocal translocation that creates the BCR-ABL chimera of the Philadelphia chromosome (Ph'), the hallmark of CML; hLH-2 was retained on the derivative 9 chromosome and is therefore centromeric of c-ABL. The proximity of hLH-2 to the breakpoint on chromosome 9 raises the possibility of cis-activation by the t(9;22)(q34;q11) translocation. In addition to finding hLH-2 expression in all cases of CML, expression was observed in lymphoid malignancies and myeloid cell lines, but not in primary cases of acute myelogenous leukaemia. The role of hLH-2 in the development or progression of leukaemia is not known. However, hLH-2 may prove useful as a marker of CML for residual disease.

Original languageEnglish
Pages (from-to)1205-1212
Number of pages8
JournalOncogene
Volume12
Issue number6
StatePublished - 8 May 1996

Keywords

  • Fluorescence in situ hybridization
  • Hematological malignancies
  • hLH-2
  • Philadelphia chromosome
  • Reverse transcription-polymerase chain reaction

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    Wu, H. K., Heng, H. H. Q., Siderovski, D. P., Dong, W. F., Okuno, Y., Shi, X. M., Tsui, L. C., & Minden, M. D. (1996). Identification of a human LIM-Hox gene, hLH-2, aberrantly expressed in chronic myelogenous leukaemia and located on 9q33-34.1. Oncogene, 12(6), 1205-1212.