Identification and characterization of the spiruchostatin biosynthetic gene cluster enable yield improvement by overexpressing a transcriptional activator

Vishwakanth Y. Potharla, Cheng Wang, Yiqiang Cheng

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Spiruchostatins A and B are members of the FK228-family of natural products with potent histone deacetylase inhibitory activities and antineoplastic activities. However, their production in the wild-type strain of Pseudomonas sp. Q71576 is low. To improve the yield, the spiruchostatin biosynthetic gene cluster (spi) was first identified by rapid genome sequencing and characterized by genetic mutations. This spi gene cluster encodes a hybrid biosynthetic pathway similar to that encoded by the FK228 biosynthetic gene cluster (dep) in Chromobacterium violaceum No. 968. Each gene cluster contains a pathway regulatory gene (spiR vs. depR), but these two genes encode transcriptional activators of different classes. Overexpression of native spiR or heterologous depR in the wild-type strain of Pseudomonas sp. Q71576 resulted in 268 or 1,285 % increase of the combined titer of spiruchostatins A and B, respectively. RT-PCR analysis indicates that overexpression of heterologous depR upregulates the expression of native spiR.

Original languageEnglish
Pages (from-to)1457-1465
Number of pages9
JournalJournal of Industrial Microbiology and Biotechnology
Volume41
Issue number9
DOIs
StatePublished - 1 Jan 2014

Fingerprint

Multigene Family
Genes
Pseudomonas
Chromobacterium
Histone Deacetylases
Biosynthetic Pathways
Regulator Genes
Biological Products
Antineoplastic Agents
Up-Regulation
Genome
Polymerase Chain Reaction
Mutation
spiruchostatin B
spiruchostatin A
romidepsin

Keywords

  • Biosynthesis
  • Genetic engineering
  • Pseudomonas sp. Q71576
  • Spiruchostatins
  • Yield improvement

Cite this

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abstract = "Spiruchostatins A and B are members of the FK228-family of natural products with potent histone deacetylase inhibitory activities and antineoplastic activities. However, their production in the wild-type strain of Pseudomonas sp. Q71576 is low. To improve the yield, the spiruchostatin biosynthetic gene cluster (spi) was first identified by rapid genome sequencing and characterized by genetic mutations. This spi gene cluster encodes a hybrid biosynthetic pathway similar to that encoded by the FK228 biosynthetic gene cluster (dep) in Chromobacterium violaceum No. 968. Each gene cluster contains a pathway regulatory gene (spiR vs. depR), but these two genes encode transcriptional activators of different classes. Overexpression of native spiR or heterologous depR in the wild-type strain of Pseudomonas sp. Q71576 resulted in 268 or 1,285 {\%} increase of the combined titer of spiruchostatins A and B, respectively. RT-PCR analysis indicates that overexpression of heterologous depR upregulates the expression of native spiR.",
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