Hypoxia, hibernation and Neuroprotection: An experimental study in mice

Changhong Ren, Sijie Li, Gary Rajah, Guo Shao, Guowei Lu, Rongrong Han, Qingjian Huang, Haiyan Li, Yuchuan Ding, Kunlin Jin, Xunming Ji

Research output: Contribution to journalArticle

Abstract

Hibernation is a unique physiological state that evolved to survive periods of food shortages. It is characterized by profound decreases in metabolic rate, body temperature and physiological functions. Studies have shown that animals in hibernation can resist neurological damage. Here, we aimed to study whether hypoxia can induce a hibernation-like state in a traditionally non-hibernating animal and whether it is neuroprotective. All procedures were conducted according to international guidelines on laboratory animal safety. Mice C57BL/6 (19-21g) were placed into a 125 mL jar with fresh air and the jar was sealed with a rubber plug. For each run, the tolerance limit was judged by the animals' appearance for ''air hunger". The animal was removed from the jar as soon as its first gasping breath appeared and was moved to another fresh-air-containing jar of similar volume. This procedure was performed in four runs. The hypoxia exposure significantly decreased oxygen (O 2 ) consumption, carbon dioxide (CO 2 ) production, respiratory rate and heart rate. Meanwhile, rectal temperature reached a minimum of 12.7±2.56°C, which is lower than a wide range of ambient temperatures. The mimicked hibernation decreased the infarct size in a focal cerebral ischemia mouse model. Our findings suggest the possibility of inducing suspended animation-like hibernation states for medical applications post injury.

Original languageEnglish
Pages (from-to)761-768
Number of pages8
JournalAging and Disease
Volume9
Issue number4
DOIs
StatePublished - 1 Jan 2018

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Hibernation
Air
Temperature
Hunger
Rubber
Laboratory Animals
Carbon Monoxide
Respiratory Rate
Brain Ischemia
Body Temperature
Inbred C57BL Mouse
Carbon Dioxide
Heart Rate
Hypoxia
Neuroprotection
Guidelines
Oxygen
Safety
Food
Wounds and Injuries

Keywords

  • Clinical application
  • Hibernation
  • Hypothermia
  • Hypoxia

Cite this

Ren, C., Li, S., Rajah, G., Shao, G., Lu, G., Han, R., ... Ji, X. (2018). Hypoxia, hibernation and Neuroprotection: An experimental study in mice. Aging and Disease, 9(4), 761-768. https://doi.org/10.14336/AD.2018.0702
Ren, Changhong ; Li, Sijie ; Rajah, Gary ; Shao, Guo ; Lu, Guowei ; Han, Rongrong ; Huang, Qingjian ; Li, Haiyan ; Ding, Yuchuan ; Jin, Kunlin ; Ji, Xunming. / Hypoxia, hibernation and Neuroprotection : An experimental study in mice. In: Aging and Disease. 2018 ; Vol. 9, No. 4. pp. 761-768.
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Ren, C, Li, S, Rajah, G, Shao, G, Lu, G, Han, R, Huang, Q, Li, H, Ding, Y, Jin, K & Ji, X 2018, 'Hypoxia, hibernation and Neuroprotection: An experimental study in mice', Aging and Disease, vol. 9, no. 4, pp. 761-768. https://doi.org/10.14336/AD.2018.0702

Hypoxia, hibernation and Neuroprotection : An experimental study in mice. / Ren, Changhong; Li, Sijie; Rajah, Gary; Shao, Guo; Lu, Guowei; Han, Rongrong; Huang, Qingjian; Li, Haiyan; Ding, Yuchuan; Jin, Kunlin; Ji, Xunming.

In: Aging and Disease, Vol. 9, No. 4, 01.01.2018, p. 761-768.

Research output: Contribution to journalArticle

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