Hypoperfusion induces overexpression of β-amyloid precursor protein mRNA in a focal ischemic rodent model

J. Shi, S. H. Yang, L. Stubley, A. L. Day, J. W. Simpkins

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118 Scopus citations

Abstract

Silent stroke is one of the risk factors of dementia. In the present study, we used a novel focal ischemic animal model to investigate the effects of comparatively small changes of cerebral blood flow (CBF) on the expression of β-amyloid precursor protein (APP) mRNA. Focal ischemia was achieved by introducing a 4-0 monofilament to the bifurcation of anterior and middle cerebral arteries. Brain samples were harvested from ischemic core and penumbra of cortices at 1, 4 and 7 days following ischemia. The expression of APP mRNA was assessed by RT-PCR. The CBF was decreased to 50% for 1 day after stroke and recovered to 90% at the fourth day after stroke. The changes of CBF were accompanied by an increase in the expression of APP mRNA. APP mRNA increased to 208% and 152% in the penumbra and core ischemic regions, respectively, on the fourth day after MCAO and remained high through the seventh day of ischemia. This study suggests brain hypoperfusion enhances APP mRNA expression and may contribute to the progression of cognitive impairment after silent stroke. Copyright (C) 2000 Elsevier Science B.V.

Original languageEnglish
Pages (from-to)1-4
Number of pages4
JournalBrain Research
Volume853
Issue number1
DOIs
StatePublished - 17 Jan 2000

Keywords

  • Amyloid precursor protein
  • Cerebral blood flow
  • Focal cerebral ischemia
  • Stroke

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