Hypochromic effect and electronic excitation energy transfer in Yt(CH2)nadenine systems

Ignacy Gryczynski, A. Kawski, S. Paszyc, B. Skalski, A. Tempczyk

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Abstract

Measurements of the UV absorption spectra of aqueous solutions of the bichromophore Yt(CH2)nadenine systems (n = 2, 3, 5 or 6) and the corresponding solutions of 1-methyladenine were carried out. All absorption spectra were subjected to a gaussian component computer analysis and the values of hypochromicity were determined for the individual electronic transitions S0 → Sn in the molecule under investigation. The strongest hypochromic effects were found in systems with n = 3. The relative fluorescence quantum yields of the systems were measured using an equimolar mixture of 1-methyl-Yt and 9-methyladenine as a reference solution for two different wavenumbers of exciting light: ν1 = 32 000 cm-1 (where only 1-methyl-Yt absorbs) and ν2 = 38 000 cm-1 (where both chromophores absorb). From these measurements, the efficiencies T of the energy transfer from adenine to the Yt base were determined as 0.41, 0.81, 0.44 and 0.17 for systems with n = 2, 3, 5 or 6 respectively. The results obtained by measuring the hypochromism and the energy transfer are in good agreement.

Original languageEnglish
Pages (from-to)153-165
Number of pages13
JournalJournal of Photochemistry
Volume30
Issue number2
DOIs
StatePublished - 1 Jan 1985

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