TY - JOUR
T1 - Hypochromic effect and electronic excitation energy transfer in Yt(CH2)nadenine systems
AU - Gryczynski, Ignacy
AU - Kawski, A.
AU - Paszyc, S.
AU - Skalski, B.
AU - Tempczyk, A.
PY - 1985/1/1
Y1 - 1985/1/1
N2 - Measurements of the UV absorption spectra of aqueous solutions of the bichromophore Yt(CH2)nadenine systems (n = 2, 3, 5 or 6) and the corresponding solutions of 1-methyladenine were carried out. All absorption spectra were subjected to a gaussian component computer analysis and the values of hypochromicity were determined for the individual electronic transitions S0 → Sn in the molecule under investigation. The strongest hypochromic effects were found in systems with n = 3. The relative fluorescence quantum yields of the systems were measured using an equimolar mixture of 1-methyl-Yt and 9-methyladenine as a reference solution for two different wavenumbers of exciting light: ν1 = 32 000 cm-1 (where only 1-methyl-Yt absorbs) and ν2 = 38 000 cm-1 (where both chromophores absorb). From these measurements, the efficiencies T of the energy transfer from adenine to the Yt base were determined as 0.41, 0.81, 0.44 and 0.17 for systems with n = 2, 3, 5 or 6 respectively. The results obtained by measuring the hypochromism and the energy transfer are in good agreement.
AB - Measurements of the UV absorption spectra of aqueous solutions of the bichromophore Yt(CH2)nadenine systems (n = 2, 3, 5 or 6) and the corresponding solutions of 1-methyladenine were carried out. All absorption spectra were subjected to a gaussian component computer analysis and the values of hypochromicity were determined for the individual electronic transitions S0 → Sn in the molecule under investigation. The strongest hypochromic effects were found in systems with n = 3. The relative fluorescence quantum yields of the systems were measured using an equimolar mixture of 1-methyl-Yt and 9-methyladenine as a reference solution for two different wavenumbers of exciting light: ν1 = 32 000 cm-1 (where only 1-methyl-Yt absorbs) and ν2 = 38 000 cm-1 (where both chromophores absorb). From these measurements, the efficiencies T of the energy transfer from adenine to the Yt base were determined as 0.41, 0.81, 0.44 and 0.17 for systems with n = 2, 3, 5 or 6 respectively. The results obtained by measuring the hypochromism and the energy transfer are in good agreement.
UR - http://www.scopus.com/inward/record.url?scp=34249873748&partnerID=8YFLogxK
U2 - 10.1016/0047-2670(85)85022-X
DO - 10.1016/0047-2670(85)85022-X
M3 - Article
AN - SCOPUS:34249873748
SN - 0047-2670
VL - 30
SP - 153
EP - 165
JO - Journal of Photochemistry
JF - Journal of Photochemistry
IS - 2
ER -