Humoral immune response induced by co-stimulatory molecule B7-2 with HPV16L1 gene

Xiangle Sun, Jianming Song, Lüsheng Si, Yili Wang, Jianfen Guo, Tianju Liu

Research output: Contribution to journalArticlepeer-review


Objective: To compare humoral immune response by co-inoculating mice with co-stimulatory molecule B7-2 and HPV16L1 recombinant plasmids and observe the neutralizing antibody activity in vitro. Methods: C57BI/6 mice were injected i. m with pcDNA-L1 + PLXHDmB7-2 plasmids. Serum IgG was detected by ELISA, Ab neutralizing protective activities were determined by Hemagglutination inhibition and HPV16-VLP binding inhibition assay. Results: Titers of serum antibody and neutralizing antibody activities were increased in HPV16L1 plus B7-2 co-immunized mice compared to those of control. Conclusion: Co-inoculation of co-stimulatory molecule B7-2 enhanced production of neutralizing antibody against specific antigen, which should be a more promising strategy for effective HPV16 prophylactic vaccine development.

Original languageEnglish
Pages (from-to)515-517
Number of pages3
JournalChinese Journal of Microbiology and Immunology
Issue number5
StatePublished - 30 Sep 2002


  • Co-stimulatory molecule
  • Human papillomavirus
  • Vaccine


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