Abstract
Objective: To compare humoral immune response by co-inoculating mice with co-stimulatory molecule B7-2 and HPV16L1 recombinant plasmids and observe the neutralizing antibody activity in vitro. Methods: C57BI/6 mice were injected i. m with pcDNA-L1 + PLXHDmB7-2 plasmids. Serum IgG was detected by ELISA, Ab neutralizing protective activities were determined by Hemagglutination inhibition and HPV16-VLP binding inhibition assay. Results: Titers of serum antibody and neutralizing antibody activities were increased in HPV16L1 plus B7-2 co-immunized mice compared to those of control. Conclusion: Co-inoculation of co-stimulatory molecule B7-2 enhanced production of neutralizing antibody against specific antigen, which should be a more promising strategy for effective HPV16 prophylactic vaccine development.
Original language | English |
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Pages (from-to) | 515-517 |
Number of pages | 3 |
Journal | Chinese Journal of Microbiology and Immunology |
Volume | 22 |
Issue number | 5 |
State | Published - 30 Sep 2002 |
Keywords
- Co-stimulatory molecule
- Human papillomavirus
- Vaccine