Humoral immune response induced by co-stimulatory molecule B7-2 with HPV16L1 gene

Xiangle Sun; Jianming Song; Lüsheng Si; Yili Wang; Jianfen Guo; Tianju Liu

Humoral immune response induced by co-stimulatory molecule B7-2 with HPV16L1 gene

Xiangle Sun, Jianming Song, Lüsheng Si, Yili Wang, Jianfen Guo, Tianju Liu

School of Biomedical Sciences

Research output: Contribution to journal › Article › peer-review

Abstract

Objective: To compare humoral immune response by co-inoculating mice with co-stimulatory molecule B7-2 and HPV16L1 recombinant plasmids and observe the neutralizing antibody activity in vitro. Methods: C57BI/6 mice were injected i. m with pcDNA-L1 + PLXHDmB7-2 plasmids. Serum IgG was detected by ELISA, Ab neutralizing protective activities were determined by Hemagglutination inhibition and HPV16-VLP binding inhibition assay. Results: Titers of serum antibody and neutralizing antibody activities were increased in HPV16L1 plus B7-2 co-immunized mice compared to those of control. Conclusion: Co-inoculation of co-stimulatory molecule B7-2 enhanced production of neutralizing antibody against specific antigen, which should be a more promising strategy for effective HPV16 prophylactic vaccine development.

Original language	English
Pages (from-to)	515-517
Number of pages	3
Journal	Chinese Journal of Microbiology and Immunology
Volume	22
Issue number	5
State	Published - 30 Sep 2002

Keywords

Co-stimulatory molecule
Human papillomavirus
Vaccine

Cite this

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title = "Humoral immune response induced by co-stimulatory molecule B7-2 with HPV16L1 gene",

abstract = "Objective: To compare humoral immune response by co-inoculating mice with co-stimulatory molecule B7-2 and HPV16L1 recombinant plasmids and observe the neutralizing antibody activity in vitro. Methods: C57BI/6 mice were injected i. m with pcDNA-L1 + PLXHDmB7-2 plasmids. Serum IgG was detected by ELISA, Ab neutralizing protective activities were determined by Hemagglutination inhibition and HPV16-VLP binding inhibition assay. Results: Titers of serum antibody and neutralizing antibody activities were increased in HPV16L1 plus B7-2 co-immunized mice compared to those of control. Conclusion: Co-inoculation of co-stimulatory molecule B7-2 enhanced production of neutralizing antibody against specific antigen, which should be a more promising strategy for effective HPV16 prophylactic vaccine development.",

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TY - JOUR

T1 - Humoral immune response induced by co-stimulatory molecule B7-2 with HPV16L1 gene

AU - Sun, Xiangle

AU - Song, Jianming

AU - Si, Lüsheng

AU - Wang, Yili

AU - Guo, Jianfen

AU - Liu, Tianju

PY - 2002/9/30

Y1 - 2002/9/30

N2 - Objective: To compare humoral immune response by co-inoculating mice with co-stimulatory molecule B7-2 and HPV16L1 recombinant plasmids and observe the neutralizing antibody activity in vitro. Methods: C57BI/6 mice were injected i. m with pcDNA-L1 + PLXHDmB7-2 plasmids. Serum IgG was detected by ELISA, Ab neutralizing protective activities were determined by Hemagglutination inhibition and HPV16-VLP binding inhibition assay. Results: Titers of serum antibody and neutralizing antibody activities were increased in HPV16L1 plus B7-2 co-immunized mice compared to those of control. Conclusion: Co-inoculation of co-stimulatory molecule B7-2 enhanced production of neutralizing antibody against specific antigen, which should be a more promising strategy for effective HPV16 prophylactic vaccine development.

AB - Objective: To compare humoral immune response by co-inoculating mice with co-stimulatory molecule B7-2 and HPV16L1 recombinant plasmids and observe the neutralizing antibody activity in vitro. Methods: C57BI/6 mice were injected i. m with pcDNA-L1 + PLXHDmB7-2 plasmids. Serum IgG was detected by ELISA, Ab neutralizing protective activities were determined by Hemagglutination inhibition and HPV16-VLP binding inhibition assay. Results: Titers of serum antibody and neutralizing antibody activities were increased in HPV16L1 plus B7-2 co-immunized mice compared to those of control. Conclusion: Co-inoculation of co-stimulatory molecule B7-2 enhanced production of neutralizing antibody against specific antigen, which should be a more promising strategy for effective HPV16 prophylactic vaccine development.

KW - Co-stimulatory molecule

KW - Human papillomavirus

KW - Vaccine

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M3 - Article

AN - SCOPUS:0037201843

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JO - Chinese Journal of Microbiology and Immunology

JF - Chinese Journal of Microbiology and Immunology

IS - 5

ER -

Humoral immune response induced by co-stimulatory molecule B7-2 with HPV16L1 gene

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