Humoral immune response and in vitro neutralizing antibody assay on co-delivery of protein HPV16L1 virus-like particle with HPV16L1 gene

Jian ming Song, Xiang Le Sun, Yi li Wang, Jian fen Guo, Tian ju Liu, Lü sheng Si

Research output: Contribution to journalArticle

1 Scopus citations

Abstract

OBJECTIVE: To compare humoral immune response by co-inoculating mice with antigen HPV16L1 virus-like particle (VLP) and HPV16L1 recombinant plasmids and then observing the neutralizing antibody activity in vitro. METHODS: C57BL/6 mice were injected intramuscularly/subcutaneously with pcDNA-L1 plasmids plus HPV16L1 VLP. Serum IgG levels were detected by ELISA, antibody neutralizing protective activities were determined by hemagglutination inhibition and HPV16L1 VLP binding inhibition assay. RESULTS: Serum antibody titers and neutralizing antibody activities were increased in HPV16L1 plasmids plus HPV16L1 VLP proteins in co-immunized mice when compared with controls. CONCLUSION: Co-inoculation of the HPV16L1 VLP protein can enhance production of neutralizing antibody activities against aimed antigen, which should be a more promising strategy for effective HPV16 prophylactic vaccine development.

Original languageEnglish
Pages (from-to)421-424
Number of pages4
JournalZhonghua bing li xue za zhi Chinese journal of pathology
Volume34
Issue number7
StatePublished - 1 Jan 2005

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