Human CD8+ T cells recognize epitopes of the 28-kDa hemolysin and the 38-kDa antigen of Mycobacterium tuberculosis

Homayoun Shams, Peter F. Barnes, Stephen E. Weis, Peter Klucar, Benjamin Wizel

Research output: Contribution to journalArticle

11 Scopus citations

Abstract

Mycobacterium tuberculosis antigens that are recognized by human CD8+ T cells are potentially important vaccine target molecules. We used a motif-based strategy to screen selected proteins of M. tuberculosis for peptides predicted to bind to human leukocyte antigen (HLA)-A*0201. We identified two 10 amino acid peptides that elicited cytolytic T lymphocyte activity and interferon-γ production by CD8+ T cells from HLA-A*0201 + healthy tuberculin reactors. These peptides were derived from the 38-kDa antigen and the 28-kDa hemolysin, the latter being a novel target for CD8+ T cells. We speculate that hemolysins may alter the phagosomal membrane surrounding intracellular M. tuberculosis, allowing themselves and other antigens to gain access to the major histocompatibility complex class I processing pathway.

Original languageEnglish
Pages (from-to)1008-1014
Number of pages7
JournalJournal of Leukocyte Biology
Volume74
Issue number6
DOIs
StatePublished - 1 Dec 2003

Keywords

  • Bacillus Calmette-Guerin
  • Cytolytic T lymphocyte
  • HLA-A*0201
  • Interferon gamma

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