Abstract
Mycobacterium tuberculosis antigens that are recognized by human CD8+ T cells are potentially important vaccine target molecules. We used a motif-based strategy to screen selected proteins of M. tuberculosis for peptides predicted to bind to human leukocyte antigen (HLA)-A*0201. We identified two 10 amino acid peptides that elicited cytolytic T lymphocyte activity and interferon-γ production by CD8+ T cells from HLA-A*0201 + healthy tuberculin reactors. These peptides were derived from the 38-kDa antigen and the 28-kDa hemolysin, the latter being a novel target for CD8+ T cells. We speculate that hemolysins may alter the phagosomal membrane surrounding intracellular M. tuberculosis, allowing themselves and other antigens to gain access to the major histocompatibility complex class I processing pathway.
Original language | English |
---|---|
Pages (from-to) | 1008-1014 |
Number of pages | 7 |
Journal | Journal of Leukocyte Biology |
Volume | 74 |
Issue number | 6 |
DOIs | |
State | Published - Dec 2003 |
Keywords
- Bacillus Calmette-Guerin
- Cytolytic T lymphocyte
- HLA-A*0201
- Interferon gamma