Human CD8+ T cells recognize epitopes of the 28-kDa hemolysin and the 38-kDa antigen of Mycobacterium tuberculosis

Homayoun Shams, Peter F. Barnes, Stephen Weis, Peter Klucar, Benjamin Wizel

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

Mycobacterium tuberculosis antigens that are recognized by human CD8+ T cells are potentially important vaccine target molecules. We used a motif-based strategy to screen selected proteins of M. tuberculosis for peptides predicted to bind to human leukocyte antigen (HLA)-A*0201. We identified two 10 amino acid peptides that elicited cytolytic T lymphocyte activity and interferon-γ production by CD8+ T cells from HLA-A*0201 + healthy tuberculin reactors. These peptides were derived from the 38-kDa antigen and the 28-kDa hemolysin, the latter being a novel target for CD8+ T cells. We speculate that hemolysins may alter the phagosomal membrane surrounding intracellular M. tuberculosis, allowing themselves and other antigens to gain access to the major histocompatibility complex class I processing pathway.

Original languageEnglish
Pages (from-to)1008-1014
Number of pages7
JournalJournal of Leukocyte Biology
Volume74
Issue number6
DOIs
StatePublished - 1 Dec 2003

Fingerprint

Hemolysin Proteins
T-Lymphocyte Epitopes
T-Lymphocytes
HLA Antigens
Mycobacterium tuberculosis
Peptides
Antigens
Intracellular Membranes
Tuberculin
Major Histocompatibility Complex
Interferons
Vaccines
Amino Acids
Mycobacterium tuberculosis antigens
Proteins

Keywords

  • Bacillus Calmette-Guerin
  • Cytolytic T lymphocyte
  • HLA-A*0201
  • Interferon gamma

Cite this

Shams, Homayoun ; Barnes, Peter F. ; Weis, Stephen ; Klucar, Peter ; Wizel, Benjamin. / Human CD8+ T cells recognize epitopes of the 28-kDa hemolysin and the 38-kDa antigen of Mycobacterium tuberculosis. In: Journal of Leukocyte Biology. 2003 ; Vol. 74, No. 6. pp. 1008-1014.
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Human CD8+ T cells recognize epitopes of the 28-kDa hemolysin and the 38-kDa antigen of Mycobacterium tuberculosis. / Shams, Homayoun; Barnes, Peter F.; Weis, Stephen; Klucar, Peter; Wizel, Benjamin.

In: Journal of Leukocyte Biology, Vol. 74, No. 6, 01.12.2003, p. 1008-1014.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Human CD8+ T cells recognize epitopes of the 28-kDa hemolysin and the 38-kDa antigen of Mycobacterium tuberculosis

AU - Shams, Homayoun

AU - Barnes, Peter F.

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AU - Wizel, Benjamin

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AB - Mycobacterium tuberculosis antigens that are recognized by human CD8+ T cells are potentially important vaccine target molecules. We used a motif-based strategy to screen selected proteins of M. tuberculosis for peptides predicted to bind to human leukocyte antigen (HLA)-A*0201. We identified two 10 amino acid peptides that elicited cytolytic T lymphocyte activity and interferon-γ production by CD8+ T cells from HLA-A*0201 + healthy tuberculin reactors. These peptides were derived from the 38-kDa antigen and the 28-kDa hemolysin, the latter being a novel target for CD8+ T cells. We speculate that hemolysins may alter the phagosomal membrane surrounding intracellular M. tuberculosis, allowing themselves and other antigens to gain access to the major histocompatibility complex class I processing pathway.

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