Horizontal polyacrylamide gel electrophoresis for the separation of DNA fragments

Hildegard Haas, Bruce Budowle, Günter Weiler

Research output: Contribution to journalArticle

36 Scopus citations

Abstract

A discontinuous borate/formate buffer system is presented for horizontal polyacrylamide gel electrophoresis of DNA fragments. The resolution potential of the system could be altered by changing the total monomer concentration (5–9%T), the concentration of the crosslinker piperazine diacrylamide (2–5%CPDA), as well as the concentration of formate in the gel (40–120 mM), the leading ion of the buffer system. The separation of DNA fragments would be improved by increasing the migration distance from 22 to 28 cm. This discontinuous polyacrylamide gel electrophoresis system proved highly reproducible.

Original languageEnglish
Pages (from-to)153-158
Number of pages6
JournalElectrophoresis
Volume15
Issue number1
DOIs
StatePublished - 1 Jan 1994

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