Histamine induced contraction of human ciliary muscle cells

Kerry L. Markwardt, Peggy E. Magnino, Iok-Hou Pang

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

In cultured human ciliary muscle cells we previously showed that histamine, via an H1 receptor, stimulates the production of inositol phosphates and mobilization of intracellular calcium. We further investigated in this study whether histamine would cause contraction of human ciliary muscle cells. Photomicrographs were taken of the ciliary muscle cells before and after exposure to histamine. Cross sectional surface area of the cells was quantified using image analysis software. A decrease in cross sectional surface area was interpreted as an indication of cell contraction. The results of this study indicated that histamine (10-6 M-10-4 M) caused contraction of human ciliary muscle cells in a concentration-dependent fashion. The effect of histamine was mediated by the H1 receptor subtype since the histamine effect was antagonized by 10-6 M chlorpheniramine (an H1 receptor subtype selective antagonist) but not by 10-4 M cimetidine (H2 antagonist) or thioperamide (H3 antagonist). The phospholipase C (PLC) inhibitor, U73122 (10-6 M) and the intracellular calcium store depleting agent thapsigargin (10-6 M) both prevented the histamine induced contraction, demonstrating that the activation of PLC and the intracellular calcium release were the key steps necessary for contraction. Our data indicate that in ciliary muscle cells, histamine, via an H1 receptor, activates PLC and increases intracellular calcium, which subsequently causes contraction of the cells.

Original languageEnglish
Pages (from-to)713-717
Number of pages5
JournalExperimental Eye Research
Volume64
Issue number5
DOIs
StatePublished - 1 Jan 1997

Fingerprint

Histamine H1 Receptors
Muscle Cells
Histamine
Type C Phospholipases
Calcium
thioperamide
Histamine Agents
Chlorpheniramine
Inositol Phosphates
Thapsigargin
Cimetidine
Software

Keywords

  • Contraction
  • H receptor
  • Histamine
  • Human ciliary muscle cells

Cite this

Markwardt, Kerry L. ; Magnino, Peggy E. ; Pang, Iok-Hou. / Histamine induced contraction of human ciliary muscle cells. In: Experimental Eye Research. 1997 ; Vol. 64, No. 5. pp. 713-717.
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Histamine induced contraction of human ciliary muscle cells. / Markwardt, Kerry L.; Magnino, Peggy E.; Pang, Iok-Hou.

In: Experimental Eye Research, Vol. 64, No. 5, 01.01.1997, p. 713-717.

Research output: Contribution to journalArticle

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