Hinf I/Tsp509 I and BsoF I polymorphisms in the flanking regions of the human VNTR locus D1S80

George T. Duncan; Kuppareddi Balamurugan; Bruce Budowle; Martin L. Tracey

doi:10.1016/S1050-3862(96)00159-3

Hinf I/Tsp509 I and BsoF I polymorphisms in the flanking regions of the human VNTR locus D1S80

George T. Duncan, Kuppareddi Balamurugan, Bruce Budowle, Martin L. Tracey

Research output: Contribution to journal › Article › peer-review

6 Scopus citations

Abstract

The minisatellite locus D1S80 (1p35-p36), is a highly polymorphic VNTR that also contains a Hinf I polymorphism in the 5' flanking region. Our data suggests that the Hinf I polymorphism is a G > T transversion 58 bases downstream from the forward primer. This G > T transversion also creates a Tsp509 I restriction site. Additionally, a G > C transversion polymorphism was identified in the 3' flanking region by the creation of a Bso F I restriction site immediately adjacent to the repeat region.

Original language	English
Pages (from-to)	119-121
Number of pages	3
Journal	Genetic Analysis - Biomolecular Engineering
Volume	13
Issue number	5
DOIs	https://doi.org/10.1016/S1050-3862(96)00159-3
State	Published - 1 Nov 1996

Keywords

D1S80
Forensics
Minisatellite
Polymorphism
Sequence
Variable number tandem repeat (VNTR)

Access to Document

10.1016/S1050-3862(96)00159-3

Fingerprint Dive into the research topics of 'Hinf I/Tsp509 I and BsoF I polymorphisms in the flanking regions of the human VNTR locus D1S80'. Together they form a unique fingerprint.

Cite this

@article{98c2216421cb4f88927e7ad948f403b5,

title = "Hinf I/Tsp509 I and BsoF I polymorphisms in the flanking regions of the human VNTR locus D1S80",

abstract = "The minisatellite locus D1S80 (1p35-p36), is a highly polymorphic VNTR that also contains a Hinf I polymorphism in the 5' flanking region. Our data suggests that the Hinf I polymorphism is a G > T transversion 58 bases downstream from the forward primer. This G > T transversion also creates a Tsp509 I restriction site. Additionally, a G > C transversion polymorphism was identified in the 3' flanking region by the creation of a Bso F I restriction site immediately adjacent to the repeat region.",

keywords = "D1S80, Forensics, Minisatellite, Polymorphism, Sequence, Variable number tandem repeat (VNTR)",

author = "Duncan, {George T.} and Kuppareddi Balamurugan and Bruce Budowle and Tracey, {Martin L.}",

year = "1996",

month = nov,

day = "1",

doi = "10.1016/S1050-3862(96)00159-3",

language = "English",

volume = "13",

pages = "119--121",

journal = "Genetic Analysis - Biomolecular Engineering",

issn = "1050-3862",

publisher = "Elsevier BV",

number = "5",

}

TY - JOUR

T1 - Hinf I/Tsp509 I and BsoF I polymorphisms in the flanking regions of the human VNTR locus D1S80

AU - Duncan, George T.

AU - Balamurugan, Kuppareddi

AU - Budowle, Bruce

AU - Tracey, Martin L.

PY - 1996/11/1

Y1 - 1996/11/1

N2 - The minisatellite locus D1S80 (1p35-p36), is a highly polymorphic VNTR that also contains a Hinf I polymorphism in the 5' flanking region. Our data suggests that the Hinf I polymorphism is a G > T transversion 58 bases downstream from the forward primer. This G > T transversion also creates a Tsp509 I restriction site. Additionally, a G > C transversion polymorphism was identified in the 3' flanking region by the creation of a Bso F I restriction site immediately adjacent to the repeat region.

AB - The minisatellite locus D1S80 (1p35-p36), is a highly polymorphic VNTR that also contains a Hinf I polymorphism in the 5' flanking region. Our data suggests that the Hinf I polymorphism is a G > T transversion 58 bases downstream from the forward primer. This G > T transversion also creates a Tsp509 I restriction site. Additionally, a G > C transversion polymorphism was identified in the 3' flanking region by the creation of a Bso F I restriction site immediately adjacent to the repeat region.

KW - D1S80

KW - Forensics

KW - Minisatellite

KW - Polymorphism

KW - Sequence

KW - Variable number tandem repeat (VNTR)

UR - http://www.scopus.com/inward/record.url?scp=16344384893&partnerID=8YFLogxK

U2 - 10.1016/S1050-3862(96)00159-3

DO - 10.1016/S1050-3862(96)00159-3

M3 - Article

C2 - 9021400

AN - SCOPUS:16344384893

VL - 13

SP - 119

EP - 121

JO - Genetic Analysis - Biomolecular Engineering

JF - Genetic Analysis - Biomolecular Engineering

SN - 1050-3862

IS - 5

ER -