High-Throughput data of circular RNA profiles in human temporal cortex tissue reveals novel insights into temporal lobe epilepsy

Jiaxin Li, Haijun Lin, Zhenrong Sun, Guanyi Kong, Xu Yan, Yujiao Wang, Xiaoxuan Wang, Yanhua Wen, Xiang Liu, Hongkun Zheng, Mei Jia, Zhongfang Shi, Rong Xu, Shaohua Yang, Fang Yuan

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Background/Aims: Circular RNAs (circRNAs) are a class of long noncoding RNAs with a closed loop structure that regulate gene expression as microRNA sponges. CircRNAs are more enriched in brain tissue, but knowledge of the role of circRNAs in temporal lobe epilepsy (TLE) has remained limited. This study is the first to identify the global expression profiles and characteristics of circRNAs in human temporal cortex tissue from TLE patients. Methods: Temporal cortices were collected from 17 TLE patients and 17 non-TLE patients. Total RNA was isolated, and high-Throughput sequencing was used to profile the transcriptome of dysregulated circRNAs. Quantitative PCR was performed for the validation of changed circRNAs. Results: In total, 78983 circRNAs, including 15.29% known and 84.71% novel circRNAs, were detected in this study. Intriguingly, 442 circRNAs were differentially expressed between the TLE and non-TLE groups (fold change≥2.0 and FDR≤0.05). Of these circRNAs, 188 were up-regulated, and 254 were down-regulated in the TLE patient group. Eight circRNAs were validated by real-Time PCR. Remarkably, circ-EFCAB2 was intensely up-regulated, while circ-DROSHA expression was significantly lower in the TLE group than in the non-TLE group (P<0.05). Bioinformatic analysis revealed that circ-EFCAB2 binds to miR-485-5p to increase the expression level of the ion channel CLCN6, while circ-DROSHA interacts with miR-1252-5p to decrease the expression level of ATP1A2. Conclusions: The dysregulations of circRNAs may reflect the pathogenesis of TLE and circ-EFCAB2 and circ-DROSHA might be potential therapeutic targets and biomarkers in TLE patients.

Original languageEnglish
Pages (from-to)677-691
Number of pages15
JournalCellular Physiology and Biochemistry
Volume45
Issue number2
DOIs
StatePublished - 1 Feb 2018

Fingerprint

Temporal Lobe Epilepsy
Temporal Lobe
Epilepsy
circular RNA
Long Noncoding RNA
Porifera
Computational Biology
MicroRNAs
Ion Channels
Transcriptome
Real-Time Polymerase Chain Reaction
Biomarkers
RNA
Gene Expression

Keywords

  • CircRNAs
  • High-Throughput sequencing
  • MicroRNA sponge
  • Temporal cortex
  • Temporal lobe epilepsy (TLE)

Cite this

Li, Jiaxin ; Lin, Haijun ; Sun, Zhenrong ; Kong, Guanyi ; Yan, Xu ; Wang, Yujiao ; Wang, Xiaoxuan ; Wen, Yanhua ; Liu, Xiang ; Zheng, Hongkun ; Jia, Mei ; Shi, Zhongfang ; Xu, Rong ; Yang, Shaohua ; Yuan, Fang. / High-Throughput data of circular RNA profiles in human temporal cortex tissue reveals novel insights into temporal lobe epilepsy. In: Cellular Physiology and Biochemistry. 2018 ; Vol. 45, No. 2. pp. 677-691.
@article{c50526d411aa4f37ab1c929c83f2c0df,
title = "High-Throughput data of circular RNA profiles in human temporal cortex tissue reveals novel insights into temporal lobe epilepsy",
abstract = "Background/Aims: Circular RNAs (circRNAs) are a class of long noncoding RNAs with a closed loop structure that regulate gene expression as microRNA sponges. CircRNAs are more enriched in brain tissue, but knowledge of the role of circRNAs in temporal lobe epilepsy (TLE) has remained limited. This study is the first to identify the global expression profiles and characteristics of circRNAs in human temporal cortex tissue from TLE patients. Methods: Temporal cortices were collected from 17 TLE patients and 17 non-TLE patients. Total RNA was isolated, and high-Throughput sequencing was used to profile the transcriptome of dysregulated circRNAs. Quantitative PCR was performed for the validation of changed circRNAs. Results: In total, 78983 circRNAs, including 15.29{\%} known and 84.71{\%} novel circRNAs, were detected in this study. Intriguingly, 442 circRNAs were differentially expressed between the TLE and non-TLE groups (fold change≥2.0 and FDR≤0.05). Of these circRNAs, 188 were up-regulated, and 254 were down-regulated in the TLE patient group. Eight circRNAs were validated by real-Time PCR. Remarkably, circ-EFCAB2 was intensely up-regulated, while circ-DROSHA expression was significantly lower in the TLE group than in the non-TLE group (P<0.05). Bioinformatic analysis revealed that circ-EFCAB2 binds to miR-485-5p to increase the expression level of the ion channel CLCN6, while circ-DROSHA interacts with miR-1252-5p to decrease the expression level of ATP1A2. Conclusions: The dysregulations of circRNAs may reflect the pathogenesis of TLE and circ-EFCAB2 and circ-DROSHA might be potential therapeutic targets and biomarkers in TLE patients.",
keywords = "CircRNAs, High-Throughput sequencing, MicroRNA sponge, Temporal cortex, Temporal lobe epilepsy (TLE)",
author = "Jiaxin Li and Haijun Lin and Zhenrong Sun and Guanyi Kong and Xu Yan and Yujiao Wang and Xiaoxuan Wang and Yanhua Wen and Xiang Liu and Hongkun Zheng and Mei Jia and Zhongfang Shi and Rong Xu and Shaohua Yang and Fang Yuan",
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Li, J, Lin, H, Sun, Z, Kong, G, Yan, X, Wang, Y, Wang, X, Wen, Y, Liu, X, Zheng, H, Jia, M, Shi, Z, Xu, R, Yang, S & Yuan, F 2018, 'High-Throughput data of circular RNA profiles in human temporal cortex tissue reveals novel insights into temporal lobe epilepsy', Cellular Physiology and Biochemistry, vol. 45, no. 2, pp. 677-691. https://doi.org/10.1159/000487161

High-Throughput data of circular RNA profiles in human temporal cortex tissue reveals novel insights into temporal lobe epilepsy. / Li, Jiaxin; Lin, Haijun; Sun, Zhenrong; Kong, Guanyi; Yan, Xu; Wang, Yujiao; Wang, Xiaoxuan; Wen, Yanhua; Liu, Xiang; Zheng, Hongkun; Jia, Mei; Shi, Zhongfang; Xu, Rong; Yang, Shaohua; Yuan, Fang.

In: Cellular Physiology and Biochemistry, Vol. 45, No. 2, 01.02.2018, p. 677-691.

Research output: Contribution to journalArticle

TY - JOUR

T1 - High-Throughput data of circular RNA profiles in human temporal cortex tissue reveals novel insights into temporal lobe epilepsy

AU - Li, Jiaxin

AU - Lin, Haijun

AU - Sun, Zhenrong

AU - Kong, Guanyi

AU - Yan, Xu

AU - Wang, Yujiao

AU - Wang, Xiaoxuan

AU - Wen, Yanhua

AU - Liu, Xiang

AU - Zheng, Hongkun

AU - Jia, Mei

AU - Shi, Zhongfang

AU - Xu, Rong

AU - Yang, Shaohua

AU - Yuan, Fang

PY - 2018/2/1

Y1 - 2018/2/1

N2 - Background/Aims: Circular RNAs (circRNAs) are a class of long noncoding RNAs with a closed loop structure that regulate gene expression as microRNA sponges. CircRNAs are more enriched in brain tissue, but knowledge of the role of circRNAs in temporal lobe epilepsy (TLE) has remained limited. This study is the first to identify the global expression profiles and characteristics of circRNAs in human temporal cortex tissue from TLE patients. Methods: Temporal cortices were collected from 17 TLE patients and 17 non-TLE patients. Total RNA was isolated, and high-Throughput sequencing was used to profile the transcriptome of dysregulated circRNAs. Quantitative PCR was performed for the validation of changed circRNAs. Results: In total, 78983 circRNAs, including 15.29% known and 84.71% novel circRNAs, were detected in this study. Intriguingly, 442 circRNAs were differentially expressed between the TLE and non-TLE groups (fold change≥2.0 and FDR≤0.05). Of these circRNAs, 188 were up-regulated, and 254 were down-regulated in the TLE patient group. Eight circRNAs were validated by real-Time PCR. Remarkably, circ-EFCAB2 was intensely up-regulated, while circ-DROSHA expression was significantly lower in the TLE group than in the non-TLE group (P<0.05). Bioinformatic analysis revealed that circ-EFCAB2 binds to miR-485-5p to increase the expression level of the ion channel CLCN6, while circ-DROSHA interacts with miR-1252-5p to decrease the expression level of ATP1A2. Conclusions: The dysregulations of circRNAs may reflect the pathogenesis of TLE and circ-EFCAB2 and circ-DROSHA might be potential therapeutic targets and biomarkers in TLE patients.

AB - Background/Aims: Circular RNAs (circRNAs) are a class of long noncoding RNAs with a closed loop structure that regulate gene expression as microRNA sponges. CircRNAs are more enriched in brain tissue, but knowledge of the role of circRNAs in temporal lobe epilepsy (TLE) has remained limited. This study is the first to identify the global expression profiles and characteristics of circRNAs in human temporal cortex tissue from TLE patients. Methods: Temporal cortices were collected from 17 TLE patients and 17 non-TLE patients. Total RNA was isolated, and high-Throughput sequencing was used to profile the transcriptome of dysregulated circRNAs. Quantitative PCR was performed for the validation of changed circRNAs. Results: In total, 78983 circRNAs, including 15.29% known and 84.71% novel circRNAs, were detected in this study. Intriguingly, 442 circRNAs were differentially expressed between the TLE and non-TLE groups (fold change≥2.0 and FDR≤0.05). Of these circRNAs, 188 were up-regulated, and 254 were down-regulated in the TLE patient group. Eight circRNAs were validated by real-Time PCR. Remarkably, circ-EFCAB2 was intensely up-regulated, while circ-DROSHA expression was significantly lower in the TLE group than in the non-TLE group (P<0.05). Bioinformatic analysis revealed that circ-EFCAB2 binds to miR-485-5p to increase the expression level of the ion channel CLCN6, while circ-DROSHA interacts with miR-1252-5p to decrease the expression level of ATP1A2. Conclusions: The dysregulations of circRNAs may reflect the pathogenesis of TLE and circ-EFCAB2 and circ-DROSHA might be potential therapeutic targets and biomarkers in TLE patients.

KW - CircRNAs

KW - High-Throughput sequencing

KW - MicroRNA sponge

KW - Temporal cortex

KW - Temporal lobe epilepsy (TLE)

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U2 - 10.1159/000487161

DO - 10.1159/000487161

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JO - Cellular Physiology and Biochemistry

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