TY - JOUR
T1 - High-field magnetic resonance imaging of the human temporal lobe
AU - Colon-Perez, Luis M.
AU - King, Michael
AU - Parekh, Mansi
AU - Boutzoukas, Angelique
AU - Carmona, Eduardo
AU - Couret, Michelle
AU - Klassen, Rosemary
AU - Mareci, Thomas H.
AU - Carney, Paul R.
N1 - Funding Information:
The support for this work was provided by the B.J. and Eve Wilder Center of Excellence for Epilepsy Research, NINDS National Institutes of Health grant RO1 NS063360 , and the North Florida/South Georgia Veterans Health System, Gainesville, FL . The contents do not represent the views of the U.S Department of Veterans Affairs or the United States Government. The authors would like to thank: Dr. Anthony Yachnis (University of Florida Neuropathology Department) for insightful discussions regarding the immunohistochemistry planning, Dr. Steven N. Roper (University of Florida Department of Neurosurgery), who performed the temporal lobe resection, and Dr. Ronald Quisling (University of Florida Radiology Department), who provided the in vivo MR images for comparison. A portion of this work was performed in the McKnight Brain Institute at the National High Magnetic Field Laboratory's AMRIS Facility, which is supported by National Science Foundation Cooperative Agreement No. DMR-1157490 , the State of Florida , and the U.S. Department of Energy . The authors would also like to thanks Prof. Marcelo Febo (University of Florida Department of Psychiatry) for insightful discussions regarding the final manuscript. The contents of this paper do not represent the views of the U.S. Department of Veterans Affairs, Department of Defense or the United States Government.
Publisher Copyright:
© 2015 The Authors. Published by Elsevier Inc. This is an open access article under the CC BY-NC-ND license.
PY - 2015/8/17
Y1 - 2015/8/17
N2 - Background Emerging high-field diffusion weighted MR imaging protocols, along with tractography, can elucidate microstructural changes associated with brain disease at the sub-millimeter image resolution. Epilepsy and other neurological disorders are accompanied by structural changes in the hippocampal formation and associated regions; however, these changes can be subtle and on a much smaller scale than the spatial resolution commonly obtained by current clinical magnetic resonance (MR) protocols in vivo. Methods We explored the possibility of studying the organization of fresh tissue with a 17.6 Tesla magnet using diffusion MR imaging and tractography. The mesoscale organization of the temporal lobe was estimated using a fresh unfixed specimen obtained from a subject who underwent anterior temporal lobectomy for medically refractory temporal lobe epilepsy (TLE). Following ex vivo imaging, the tissue was fixed, serial-sectioned, and stained for correlation with imaging. Findings We resolved tissue microstructural organizational features in the temporal lobe from diffusion MR imaging and tractography in fresh tissue. Conclusions Fresh ex vivo MR imaging, along with tractography, revealed complex intra-temporal structural variation corresponding to neuronal cell body layers, dendritic fields, and axonal projection systems evident histologically. This is the first study to describe in detail the human temporal lobe structural organization using high-field MR imaging and tractography. By preserving the 3-dimensional structures of the hippocampus and surrounding structures, specific changes in anatomy may inform us about the changes that occur in TLE in relation to the disease process and structural underpinnings in epilepsy-related memory dysfunction.
AB - Background Emerging high-field diffusion weighted MR imaging protocols, along with tractography, can elucidate microstructural changes associated with brain disease at the sub-millimeter image resolution. Epilepsy and other neurological disorders are accompanied by structural changes in the hippocampal formation and associated regions; however, these changes can be subtle and on a much smaller scale than the spatial resolution commonly obtained by current clinical magnetic resonance (MR) protocols in vivo. Methods We explored the possibility of studying the organization of fresh tissue with a 17.6 Tesla magnet using diffusion MR imaging and tractography. The mesoscale organization of the temporal lobe was estimated using a fresh unfixed specimen obtained from a subject who underwent anterior temporal lobectomy for medically refractory temporal lobe epilepsy (TLE). Following ex vivo imaging, the tissue was fixed, serial-sectioned, and stained for correlation with imaging. Findings We resolved tissue microstructural organizational features in the temporal lobe from diffusion MR imaging and tractography in fresh tissue. Conclusions Fresh ex vivo MR imaging, along with tractography, revealed complex intra-temporal structural variation corresponding to neuronal cell body layers, dendritic fields, and axonal projection systems evident histologically. This is the first study to describe in detail the human temporal lobe structural organization using high-field MR imaging and tractography. By preserving the 3-dimensional structures of the hippocampus and surrounding structures, specific changes in anatomy may inform us about the changes that occur in TLE in relation to the disease process and structural underpinnings in epilepsy-related memory dysfunction.
KW - DWI
KW - Ex vivo brain imaging
KW - High-field MRI
KW - Mesoscale structure
KW - Tractography
UR - http://www.scopus.com/inward/record.url?scp=84939123632&partnerID=8YFLogxK
U2 - 10.1016/j.nicl.2015.07.005
DO - 10.1016/j.nicl.2015.07.005
M3 - Article
C2 - 26413472
AN - SCOPUS:84939123632
SN - 2213-1582
VL - 9
SP - 58
EP - 68
JO - NeuroImage: Clinical
JF - NeuroImage: Clinical
ER -