High-affinity immobilization of proteins using biotin- and GST-based coupling strategies.

Stephanie Q. Hutsell, Randall J. Kimple, David P. Siderovski, Francis S. Willard, Adam J. Kimple

Research output: Contribution to journalArticlepeer-review

31 Scopus citations

Abstract

Surface plasmon resonance (SPR) is a highly sensitive method for the detection of molecular interactions. One interacting partner is immobilized on the sensor chip surface while the other is injected across the sensor surface. This chapter focuses on high-affinity immobilization of protein substrates for affinity and kinetic analyses using biotin/streptavidin interaction and GST/anti-GST-antibody interaction.

Original languageEnglish
Pages (from-to)75-90
Number of pages16
JournalMethods in molecular biology (Clifton, N.J.)
Volume627
DOIs
StatePublished - 2010

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