High-affinity immobilization of proteins using biotin- and GST-based coupling strategies.

Stephanie Q. Hutsell; Randall J. Kimple; David P. Siderovski; Francis S. Willard; Adam J. Kimple

doi:10.1007/978-1-60761-670-2_4

High-affinity immobilization of proteins using biotin- and GST-based coupling strategies.

Stephanie Q. Hutsell, Randall J. Kimple, David P. Siderovski, Francis S. Willard, Adam J. Kimple

Research output: Contribution to journal › Article › peer-review

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Abstract

Surface plasmon resonance (SPR) is a highly sensitive method for the detection of molecular interactions. One interacting partner is immobilized on the sensor chip surface while the other is injected across the sensor surface. This chapter focuses on high-affinity immobilization of protein substrates for affinity and kinetic analyses using biotin/streptavidin interaction and GST/anti-GST-antibody interaction.

Original language	English
Pages (from-to)	75-90
Number of pages	16
Journal	Methods in molecular biology (Clifton, N.J.)
Volume	627
DOIs	https://doi.org/10.1007/978-1-60761-670-2_4
State	Published - 2010

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abstract = "Surface plasmon resonance (SPR) is a highly sensitive method for the detection of molecular interactions. One interacting partner is immobilized on the sensor chip surface while the other is injected across the sensor surface. This chapter focuses on high-affinity immobilization of protein substrates for affinity and kinetic analyses using biotin/streptavidin interaction and GST/anti-GST-antibody interaction.",

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AU - Willard, Francis S.

AU - Kimple, Adam J.

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AB - Surface plasmon resonance (SPR) is a highly sensitive method for the detection of molecular interactions. One interacting partner is immobilized on the sensor chip surface while the other is injected across the sensor surface. This chapter focuses on high-affinity immobilization of protein substrates for affinity and kinetic analyses using biotin/streptavidin interaction and GST/anti-GST-antibody interaction.

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ER -

High-affinity immobilization of proteins using biotin- and GST-based coupling strategies.

Abstract

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