Heterozygosity of genes on the sex chromosome regulates Candida albicans virulence

Wei Wu, Shawn R. Lockhart, Claude Pujol, Srikantha Thyagarajan, David R. Soll

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52 Citations (Scopus)

Abstract

In the mouse model for systemic infection, natural a/α strains of C. albicans are more virulent and more competitive than their spontaneous MTL-homozygous offspring, which arise primarily by loss of one chromosome 5 homologue followed by duplication of the retained homologue (uniparental disomy). Deletion of either the a or α copy of the MTL locus of natural a/α strains results in a small decrease in virulence, and a small decrease in competitiveness. Loss of the heterozygosity of non-MTL genes along chromosome 5, however, results in larger decreases in virulence and competitiveness. Natural MTL-homozygous strains are on average less virulent than natural MTL-heterozygous strains and arise by multiple mitotic cross-overs along chromosome 5 outside of the MTL region. These results are consistent with the hypothesis that a competitive advantage of natural a/α strains over MTL-homozygous offspring maintains the mating system of C. albicans.

Original languageEnglish
Pages (from-to)1587-1604
Number of pages18
JournalMolecular Microbiology
Volume64
Issue number6
DOIs
StatePublished - 1 Jun 2007

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Chromosomes, Human, Pair 5
Sex Chromosomes
Candida albicans
Virulence
Uniparental Disomy
Genes
Loss of Heterozygosity
Infection

Cite this

Wu, Wei ; Lockhart, Shawn R. ; Pujol, Claude ; Thyagarajan, Srikantha ; Soll, David R. / Heterozygosity of genes on the sex chromosome regulates Candida albicans virulence. In: Molecular Microbiology. 2007 ; Vol. 64, No. 6. pp. 1587-1604.
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Heterozygosity of genes on the sex chromosome regulates Candida albicans virulence. / Wu, Wei; Lockhart, Shawn R.; Pujol, Claude; Thyagarajan, Srikantha; Soll, David R.

In: Molecular Microbiology, Vol. 64, No. 6, 01.06.2007, p. 1587-1604.

Research output: Contribution to journalArticleResearchpeer-review

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AB - In the mouse model for systemic infection, natural a/α strains of C. albicans are more virulent and more competitive than their spontaneous MTL-homozygous offspring, which arise primarily by loss of one chromosome 5 homologue followed by duplication of the retained homologue (uniparental disomy). Deletion of either the a or α copy of the MTL locus of natural a/α strains results in a small decrease in virulence, and a small decrease in competitiveness. Loss of the heterozygosity of non-MTL genes along chromosome 5, however, results in larger decreases in virulence and competitiveness. Natural MTL-homozygous strains are on average less virulent than natural MTL-heterozygous strains and arise by multiple mitotic cross-overs along chromosome 5 outside of the MTL region. These results are consistent with the hypothesis that a competitive advantage of natural a/α strains over MTL-homozygous offspring maintains the mating system of C. albicans.

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