Heterogeneity of dog interstitial fluid (peripheral lymph) high density lipoproteins: Implications for a role in reverse cholesterol transport

Ladislav Dory, L. M. Boquet, R. L. Hamilton, C. H. Sloop, P. S. Roheim

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Abstract

The heterogeneity of dog interstitial fluid (peripheral lymph) high density lipoprotein (HDL) was investigated and compared to plasma HDL. Interstitial fluid and plasma HDL of normal and cholesterol-fed dogs was subfractionated by ultracentrifugation and affinity and molecular weight sieving chromatography. Both plasma (P) and interstitial fluid (L) HDL can be subfractionated into a larger fraction (P-I and L-I) and a smaller one (P-II and L-II). Cholesterol feeding induces a large increase in the P-I and L-I component of HDL, but the increase in L-I is far greater in proportion than that of P-I. Furthermore, L-I of cholesterol-fed dogs appears to be almost exclusively discoid in shape, while only approximately 15% of particles in P-I are discoidal. The discoid HDL of L-I is reflected in its chemical composition: 28% unesterified cholesterol, 6% cholesteryl ester, 45% phospholipid, and 21% protein. It contains large amounts of apoE in addition to apoA-I and apoA-IV. We found that the association of apoE with discoid particles is frequent, but not necessary. Calculations based on known protein mass and quantitation of discoid particles on electron micrographs suggest that the concentration of discoid particles in the peripheral lymph of cholesterol-fed dogs is about fourfold that of the plasma of the same animal. These findings provide strong circumstantial evidence for the peripheral formation of discoid HDL, perhaps as an early event in reverse cholesterol transport.

Original languageEnglish
Pages (from-to)519-527
Number of pages9
JournalJournal of Lipid Research
Volume26
Issue number5
StatePublished - 19 Sep 1985

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Extracellular Fluid
Lymph
HDL Lipoproteins
Cholesterol
Dogs
Fluids
Plasmas
Apolipoproteins E
Apolipoproteins A
Cholesterol Esters
Ultracentrifugation
Apolipoprotein A-I
HDL Cholesterol
Chromatography
Phospholipids
Proteins
Molecular Weight
Electrons
Animals
Molecular weight

Cite this

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title = "Heterogeneity of dog interstitial fluid (peripheral lymph) high density lipoproteins: Implications for a role in reverse cholesterol transport",
abstract = "The heterogeneity of dog interstitial fluid (peripheral lymph) high density lipoprotein (HDL) was investigated and compared to plasma HDL. Interstitial fluid and plasma HDL of normal and cholesterol-fed dogs was subfractionated by ultracentrifugation and affinity and molecular weight sieving chromatography. Both plasma (P) and interstitial fluid (L) HDL can be subfractionated into a larger fraction (P-I and L-I) and a smaller one (P-II and L-II). Cholesterol feeding induces a large increase in the P-I and L-I component of HDL, but the increase in L-I is far greater in proportion than that of P-I. Furthermore, L-I of cholesterol-fed dogs appears to be almost exclusively discoid in shape, while only approximately 15{\%} of particles in P-I are discoidal. The discoid HDL of L-I is reflected in its chemical composition: 28{\%} unesterified cholesterol, 6{\%} cholesteryl ester, 45{\%} phospholipid, and 21{\%} protein. It contains large amounts of apoE in addition to apoA-I and apoA-IV. We found that the association of apoE with discoid particles is frequent, but not necessary. Calculations based on known protein mass and quantitation of discoid particles on electron micrographs suggest that the concentration of discoid particles in the peripheral lymph of cholesterol-fed dogs is about fourfold that of the plasma of the same animal. These findings provide strong circumstantial evidence for the peripheral formation of discoid HDL, perhaps as an early event in reverse cholesterol transport.",
author = "Ladislav Dory and Boquet, {L. M.} and Hamilton, {R. L.} and Sloop, {C. H.} and Roheim, {P. S.}",
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Heterogeneity of dog interstitial fluid (peripheral lymph) high density lipoproteins : Implications for a role in reverse cholesterol transport. / Dory, Ladislav; Boquet, L. M.; Hamilton, R. L.; Sloop, C. H.; Roheim, P. S.

In: Journal of Lipid Research, Vol. 26, No. 5, 19.09.1985, p. 519-527.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Heterogeneity of dog interstitial fluid (peripheral lymph) high density lipoproteins

T2 - Implications for a role in reverse cholesterol transport

AU - Dory, Ladislav

AU - Boquet, L. M.

AU - Hamilton, R. L.

AU - Sloop, C. H.

AU - Roheim, P. S.

PY - 1985/9/19

Y1 - 1985/9/19

N2 - The heterogeneity of dog interstitial fluid (peripheral lymph) high density lipoprotein (HDL) was investigated and compared to plasma HDL. Interstitial fluid and plasma HDL of normal and cholesterol-fed dogs was subfractionated by ultracentrifugation and affinity and molecular weight sieving chromatography. Both plasma (P) and interstitial fluid (L) HDL can be subfractionated into a larger fraction (P-I and L-I) and a smaller one (P-II and L-II). Cholesterol feeding induces a large increase in the P-I and L-I component of HDL, but the increase in L-I is far greater in proportion than that of P-I. Furthermore, L-I of cholesterol-fed dogs appears to be almost exclusively discoid in shape, while only approximately 15% of particles in P-I are discoidal. The discoid HDL of L-I is reflected in its chemical composition: 28% unesterified cholesterol, 6% cholesteryl ester, 45% phospholipid, and 21% protein. It contains large amounts of apoE in addition to apoA-I and apoA-IV. We found that the association of apoE with discoid particles is frequent, but not necessary. Calculations based on known protein mass and quantitation of discoid particles on electron micrographs suggest that the concentration of discoid particles in the peripheral lymph of cholesterol-fed dogs is about fourfold that of the plasma of the same animal. These findings provide strong circumstantial evidence for the peripheral formation of discoid HDL, perhaps as an early event in reverse cholesterol transport.

AB - The heterogeneity of dog interstitial fluid (peripheral lymph) high density lipoprotein (HDL) was investigated and compared to plasma HDL. Interstitial fluid and plasma HDL of normal and cholesterol-fed dogs was subfractionated by ultracentrifugation and affinity and molecular weight sieving chromatography. Both plasma (P) and interstitial fluid (L) HDL can be subfractionated into a larger fraction (P-I and L-I) and a smaller one (P-II and L-II). Cholesterol feeding induces a large increase in the P-I and L-I component of HDL, but the increase in L-I is far greater in proportion than that of P-I. Furthermore, L-I of cholesterol-fed dogs appears to be almost exclusively discoid in shape, while only approximately 15% of particles in P-I are discoidal. The discoid HDL of L-I is reflected in its chemical composition: 28% unesterified cholesterol, 6% cholesteryl ester, 45% phospholipid, and 21% protein. It contains large amounts of apoE in addition to apoA-I and apoA-IV. We found that the association of apoE with discoid particles is frequent, but not necessary. Calculations based on known protein mass and quantitation of discoid particles on electron micrographs suggest that the concentration of discoid particles in the peripheral lymph of cholesterol-fed dogs is about fourfold that of the plasma of the same animal. These findings provide strong circumstantial evidence for the peripheral formation of discoid HDL, perhaps as an early event in reverse cholesterol transport.

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