Heat Shock Protein 47 Promotes Glioma Angiogenesis

Zhe Bao Wu, Lin Cai, Shao Jian Lin, Zhi Gen Leng, Yu Hang Guo, Wen Lei Yang, Yi Wei Chu, Shaohua Yang, Wei Guo Zhao

Research output: Contribution to journalArticleResearchpeer-review

11 Citations (Scopus)

Abstract

Heat shock protein 47 (HSP47) is a collagen-binding protein, which has been recently found to express in glioma vessels. However, the expression profile of HSP47 in glioma patients and the underlying mechanisms of HSP47 on glioma angiogenesis are not fully explored. In the current study, we found that expression of HSP47 in glioma vessels was correlated with the grades of gliomas. HSP47 knockdown by siRNAs significantly decreased cell viability in vitro and tumor volume in vivo; moreover, it reduced the microvessel density (MVD) by CD31 immunohistochemistry in vivo. HSP47 knockdown significantly inhibited tube formation, invasion and proliferation of human umbilical vein endothelial cells (HUVECs). Furthermore, conditional medium derived from HSP47 knockdown cells significantly inhibited HUVECs tube formation and migration, while it increased chemosensitivity of HUVECs cells to Avastin. Silencing of HSP47 decreased VEGF expression in glioma cells consistently, and reduced glioma vasculature. Furthermore, HSP47 promoted glioma angiogenesis through HIF1α-VEGFR2 signaling. The present study demonstrates that HSP47 promotes glioma angiogenesis and highlights the importance of HSP47 as an attractive therapeutic target of GBM.

Original languageEnglish
Pages (from-to)31-42
Number of pages12
JournalBrain Pathology
Volume26
Issue number1
DOIs
StatePublished - 1 Jan 2016

Fingerprint

HSP47 Heat-Shock Proteins
Glioma
Human Umbilical Vein Endothelial Cells
Microvessels
Tumor Burden
Vascular Endothelial Growth Factor A

Keywords

  • VEGFR2
  • angiogenesis
  • glioblastoma
  • glioma
  • heat shock protein 47

Cite this

Wu, Z. B., Cai, L., Lin, S. J., Leng, Z. G., Guo, Y. H., Yang, W. L., ... Zhao, W. G. (2016). Heat Shock Protein 47 Promotes Glioma Angiogenesis. Brain Pathology, 26(1), 31-42. https://doi.org/10.1111/bpa.12256
Wu, Zhe Bao ; Cai, Lin ; Lin, Shao Jian ; Leng, Zhi Gen ; Guo, Yu Hang ; Yang, Wen Lei ; Chu, Yi Wei ; Yang, Shaohua ; Zhao, Wei Guo. / Heat Shock Protein 47 Promotes Glioma Angiogenesis. In: Brain Pathology. 2016 ; Vol. 26, No. 1. pp. 31-42.
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Wu, ZB, Cai, L, Lin, SJ, Leng, ZG, Guo, YH, Yang, WL, Chu, YW, Yang, S & Zhao, WG 2016, 'Heat Shock Protein 47 Promotes Glioma Angiogenesis', Brain Pathology, vol. 26, no. 1, pp. 31-42. https://doi.org/10.1111/bpa.12256

Heat Shock Protein 47 Promotes Glioma Angiogenesis. / Wu, Zhe Bao; Cai, Lin; Lin, Shao Jian; Leng, Zhi Gen; Guo, Yu Hang; Yang, Wen Lei; Chu, Yi Wei; Yang, Shaohua; Zhao, Wei Guo.

In: Brain Pathology, Vol. 26, No. 1, 01.01.2016, p. 31-42.

Research output: Contribution to journalArticleResearchpeer-review

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Wu ZB, Cai L, Lin SJ, Leng ZG, Guo YH, Yang WL et al. Heat Shock Protein 47 Promotes Glioma Angiogenesis. Brain Pathology. 2016 Jan 1;26(1):31-42. https://doi.org/10.1111/bpa.12256