TY - JOUR
T1 - Haemocompatibility improvement of metallic surfaces by covalent immobilization of heparin-liposomes
AU - Kastellorizios, Michail
AU - Michanetzis, Georgios P.A.K.
AU - Pistillo, Bianca Rita
AU - Mourtas, Spyridon
AU - Klepetsanis, Pavlos
AU - Favia, Piero
AU - Sardella, Eloisa
AU - D'Agostino, Ricardo
AU - Missirlis, Yannis F.
AU - Antimisiaris, Sophia G.
PY - 2012/8/1
Y1 - 2012/8/1
N2 - Stainless steel surfaces were processed by means of plasma enhanced chemical vapor deposition (PE-CVD) fed with acrylic acid vapors in order to functionalize them with carboxyl groups, which were subsequently activated for covalent immobilization of heparin-loaded (HEP) NH 2 group-functionalized (Fun) nanoliposomes (NLs). Empty Fun or HEP non-functionalized (control) NLs were used as controls. NLs were characterized for mean diameter, surface charge and heparin encapsulation/release. Different lipid compositions were used for NL construction; PC/Chol (2:1 mol/mol) or PC/Chol (4:1 mol/mol) (fluid type vesicles) [which allow gradual release of heparin] and DSPC/Chol (2:1 mol/mol) (rigid type vesicles). Surface haemocompatibility was tested by measuring blood clotting time. Platelet adhesion on surfaces was evaluated morphologically by SEM and CLSM. The haemocompatibility of plasma-processed surfaces was improved (compared to untreated surfaces); Fun-HEP NL-coated surfaces demonstrated highest coagulation times. For short surface/blood incubation periods, surfaces coated with Fun-HEP NLs consisting of PC/Chol (2:1) had higher coagulation times (compared to DSPC/Chol NLs) due to faster release of heparin. Heparin release rate from the various NL types and surface platelet adhesion results were in agreement with the corresponding blood coagulation times. Concluding, covalent immobilization of drug entrapping NLs on plasma processed surfaces is a potential method for preparation of controlled-rate drug-eluting metallic stents or devices.
AB - Stainless steel surfaces were processed by means of plasma enhanced chemical vapor deposition (PE-CVD) fed with acrylic acid vapors in order to functionalize them with carboxyl groups, which were subsequently activated for covalent immobilization of heparin-loaded (HEP) NH 2 group-functionalized (Fun) nanoliposomes (NLs). Empty Fun or HEP non-functionalized (control) NLs were used as controls. NLs were characterized for mean diameter, surface charge and heparin encapsulation/release. Different lipid compositions were used for NL construction; PC/Chol (2:1 mol/mol) or PC/Chol (4:1 mol/mol) (fluid type vesicles) [which allow gradual release of heparin] and DSPC/Chol (2:1 mol/mol) (rigid type vesicles). Surface haemocompatibility was tested by measuring blood clotting time. Platelet adhesion on surfaces was evaluated morphologically by SEM and CLSM. The haemocompatibility of plasma-processed surfaces was improved (compared to untreated surfaces); Fun-HEP NL-coated surfaces demonstrated highest coagulation times. For short surface/blood incubation periods, surfaces coated with Fun-HEP NLs consisting of PC/Chol (2:1) had higher coagulation times (compared to DSPC/Chol NLs) due to faster release of heparin. Heparin release rate from the various NL types and surface platelet adhesion results were in agreement with the corresponding blood coagulation times. Concluding, covalent immobilization of drug entrapping NLs on plasma processed surfaces is a potential method for preparation of controlled-rate drug-eluting metallic stents or devices.
KW - Controlled drug release
KW - Drug delivery
KW - Haemocompatibility
KW - Heparin
KW - Liposomes
KW - Plasma processing
KW - Stent
UR - http://www.scopus.com/inward/record.url?scp=84861626008&partnerID=8YFLogxK
U2 - 10.1016/j.ijpharm.2012.04.057
DO - 10.1016/j.ijpharm.2012.04.057
M3 - Article
C2 - 22569232
AN - SCOPUS:84861626008
SN - 0378-5173
VL - 432
SP - 91
EP - 98
JO - International Journal of Pharmaceutics
JF - International Journal of Pharmaceutics
IS - 1-2
ER -