Hae III - A suitable restriction endonuclease for restriction fragment length polymorphism analysis of biological evidence samples

Bruce Budowle; J. S. Waye; G. G. Shutler; F. S. Baechtel

Hae III - A suitable restriction endonuclease for restriction fragment length polymorphism analysis of biological evidence samples

Bruce Budowle, J. S. Waye, G. G. Shutler, F. S. Baechtel

Research output: Contribution to journal › Article › peer-review

Abstract

Hae III has been selected by our laboratories as the restriction endonuclease of choice for restriction fragment length polymorphism analysis of forensic science samples. The enzyme is compatible with the D2S44 probe system and generates relatively small DNA fragments for that marker system. Similarly, Hae III is compatible with several other independent polymorphic loci, including D1S7, D4S139, D16S85, D17S74, D17S79, D14S13, and D20S15. Hae III is functional under a variety of adverse conditions for DNA digestion and is not affected by the methylation pattern in mammals. Fanally, Hae III is a relatively inexpensive restriction endonuclease.

Original language	English
Pages (from-to)	530-536
Number of pages	7
Journal	Journal of Forensic Sciences
Volume	35
Issue number	3
State	Published - 1 Jan 1990

Keywords

Biology and pathology
D2S44 (YNH24)
Deoxyribonucleic acid (DNA)
Enzymes
Hae III
Methylation
Restriction endonuclease
Restriction fragment length polymorphism
Variable number of tandem repeats

Cite this

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title = "Hae III - A suitable restriction endonuclease for restriction fragment length polymorphism analysis of biological evidence samples",

abstract = "Hae III has been selected by our laboratories as the restriction endonuclease of choice for restriction fragment length polymorphism analysis of forensic science samples. The enzyme is compatible with the D2S44 probe system and generates relatively small DNA fragments for that marker system. Similarly, Hae III is compatible with several other independent polymorphic loci, including D1S7, D4S139, D16S85, D17S74, D17S79, D14S13, and D20S15. Hae III is functional under a variety of adverse conditions for DNA digestion and is not affected by the methylation pattern in mammals. Fanally, Hae III is a relatively inexpensive restriction endonuclease.",

keywords = "Biology and pathology, D2S44 (YNH24), Deoxyribonucleic acid (DNA), Enzymes, Hae III, Methylation, Restriction endonuclease, Restriction fragment length polymorphism, Variable number of tandem repeats",

author = "Bruce Budowle and Waye, {J. S.} and Shutler, {G. G.} and Baechtel, {F. S.}",

year = "1990",

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language = "English",

volume = "35",

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journal = "Journal of forensic sciences",

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TY - JOUR

T1 - Hae III - A suitable restriction endonuclease for restriction fragment length polymorphism analysis of biological evidence samples

AU - Budowle, Bruce

AU - Waye, J. S.

AU - Shutler, G. G.

AU - Baechtel, F. S.

PY - 1990/1/1

Y1 - 1990/1/1

N2 - Hae III has been selected by our laboratories as the restriction endonuclease of choice for restriction fragment length polymorphism analysis of forensic science samples. The enzyme is compatible with the D2S44 probe system and generates relatively small DNA fragments for that marker system. Similarly, Hae III is compatible with several other independent polymorphic loci, including D1S7, D4S139, D16S85, D17S74, D17S79, D14S13, and D20S15. Hae III is functional under a variety of adverse conditions for DNA digestion and is not affected by the methylation pattern in mammals. Fanally, Hae III is a relatively inexpensive restriction endonuclease.

AB - Hae III has been selected by our laboratories as the restriction endonuclease of choice for restriction fragment length polymorphism analysis of forensic science samples. The enzyme is compatible with the D2S44 probe system and generates relatively small DNA fragments for that marker system. Similarly, Hae III is compatible with several other independent polymorphic loci, including D1S7, D4S139, D16S85, D17S74, D17S79, D14S13, and D20S15. Hae III is functional under a variety of adverse conditions for DNA digestion and is not affected by the methylation pattern in mammals. Fanally, Hae III is a relatively inexpensive restriction endonuclease.

KW - Biology and pathology

KW - D2S44 (YNH24)

KW - Deoxyribonucleic acid (DNA)

KW - Enzymes

KW - Hae III

KW - Methylation

KW - Restriction endonuclease

KW - Restriction fragment length polymorphism

KW - Variable number of tandem repeats

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M3 - Article

C2 - 1971850

AN - SCOPUS:0025308330

SN - 0022-1198

VL - 35

SP - 530

EP - 536

JO - Journal of forensic sciences

JF - Journal of forensic sciences

IS - 3

ER -

Hae III - A suitable restriction endonuclease for restriction fragment length polymorphism analysis of biological evidence samples

Abstract

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