Hae III - A suitable restriction endonuclease for restriction fragment length polymorphism analysis of biological evidence samples

Bruce Budowle, J. S. Waye, G. G. Shutler, F. S. Baechtel

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31 Scopus citations

Abstract

Hae III has been selected by our laboratories as the restriction endonuclease of choice for restriction fragment length polymorphism analysis of forensic science samples. The enzyme is compatible with the D2S44 probe system and generates relatively small DNA fragments for that marker system. Similarly, Hae III is compatible with several other independent polymorphic loci, including D1S7, D4S139, D16S85, D17S74, D17S79, D14S13, and D20S15. Hae III is functional under a variety of adverse conditions for DNA digestion and is not affected by the methylation pattern in mammals. Fanally, Hae III is a relatively inexpensive restriction endonuclease.

Original languageEnglish
Pages (from-to)530-536
Number of pages7
JournalJournal of Forensic Sciences
Volume35
Issue number3
StatePublished - 1 Jan 1990

Keywords

  • Biology and pathology
  • D2S44 (YNH24)
  • Deoxyribonucleic acid (DNA)
  • Enzymes
  • Hae III
  • Methylation
  • Restriction endonuclease
  • Restriction fragment length polymorphism
  • Variable number of tandem repeats

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