TY - JOUR
T1 - Green Tea Polyphenol Prevents Diabetic Rats from Acute Kidney Injury after Cardiopulmonary Bypass Presented at the American Heart Association Scientific Session, Chicago, IL, Nov 15-19, 2014.
AU - Funamoto, Masaki
AU - Masumoto, Hidetoshi
AU - Takaori, Koji
AU - Taki, Tomofumi
AU - Setozaki, Shuji
AU - Yamazaki, Kazuhiro
AU - Minakata, Kenji
AU - Ikeda, Tadashi
AU - Hyon, Suong Hyu
AU - Sakata, Ryuzo
N1 - Funding Information:
This work was supported by research grants from the Ministry of Education , Culture, Sports, Science and Technology and the Ministry of Health , Labor and Welfare, Japan (to R.S.), and Invited Research Project of Institute for Advancement of Clinical and Translational Science, Kyoto University Hospital (to R.S.). The authors wish to thank Dr Motoko Yanagita for logistic support, Ms Fumiyo Kataoka and Mr Shuichi Miyake for their expert technical assistance, Mr Yuki Gen (BioVerde) for providing the EGCG, Mr Gen Yoshida (MERA) for manufacturing development of the CPB circuit and oxygenator, and Dr William J. Kowalski (University of Louisville) for critical reading of the manuscript.
Publisher Copyright:
© 2016 The Society of Thoracic Surgeons.
PY - 2016/4/1
Y1 - 2016/4/1
N2 - Background Acute kidney injury (AKI) is a common complication accompanying cardiopulmonary bypass (CPB) and is independently associated with increased morbidity and death. Diabetes mellitus increases the risk for AKI after CPB. Epigallocatechin-3-gallate (EGCG) is a major component of the polyphenolic fraction of green tea, which possesses cardioprotective activities, as previously reported. We hypothesized that EGCG also possesses a renoprotective effect through its diverse biochemical properties and assessed the effect on renal function after CPB for diabetic rats. Methods Goto-Kakizaki rats developing type 2 diabetes mellitus were randomly assigned to one of the following groups: sham (n = 10), CPB (CPB alone, n = 9), or EGCG (CPB + EGCG, n = 10). CPB was conducted for 30 minutes at a flow rate of 100 mL/kg/min in the CPB and EGCG groups. Rats assigned to the EGCG group were administrated EGCG solution orally for 2 weeks before CPB. We evaluated renal biochemical or histologic changes at 24 hours after CPB. Results Compared with the CPB group, the EGCG group exhibited milder tubular injury histologically (p < 0.0001) and reduced expression of kidney injury molecule-1, a biomarker for renal tubular injury (p < 0.0001) and 8-hydroxy-2′-deoxyguanosine (p < 0.01), indicating attenuated oxidant stress. Conclusions Preoperative oral administration of EGCG ameliorates AKI in a CPB model of diabetic rats through antioxidative properties. This simple method could be applied in a clinical setting as a prophylactic renal protection against AKI after CPB, especially for high-risk patients with diabetes mellitus.
AB - Background Acute kidney injury (AKI) is a common complication accompanying cardiopulmonary bypass (CPB) and is independently associated with increased morbidity and death. Diabetes mellitus increases the risk for AKI after CPB. Epigallocatechin-3-gallate (EGCG) is a major component of the polyphenolic fraction of green tea, which possesses cardioprotective activities, as previously reported. We hypothesized that EGCG also possesses a renoprotective effect through its diverse biochemical properties and assessed the effect on renal function after CPB for diabetic rats. Methods Goto-Kakizaki rats developing type 2 diabetes mellitus were randomly assigned to one of the following groups: sham (n = 10), CPB (CPB alone, n = 9), or EGCG (CPB + EGCG, n = 10). CPB was conducted for 30 minutes at a flow rate of 100 mL/kg/min in the CPB and EGCG groups. Rats assigned to the EGCG group were administrated EGCG solution orally for 2 weeks before CPB. We evaluated renal biochemical or histologic changes at 24 hours after CPB. Results Compared with the CPB group, the EGCG group exhibited milder tubular injury histologically (p < 0.0001) and reduced expression of kidney injury molecule-1, a biomarker for renal tubular injury (p < 0.0001) and 8-hydroxy-2′-deoxyguanosine (p < 0.01), indicating attenuated oxidant stress. Conclusions Preoperative oral administration of EGCG ameliorates AKI in a CPB model of diabetic rats through antioxidative properties. This simple method could be applied in a clinical setting as a prophylactic renal protection against AKI after CPB, especially for high-risk patients with diabetes mellitus.
UR - http://www.scopus.com/inward/record.url?scp=84949637916&partnerID=8YFLogxK
U2 - 10.1016/j.athoracsur.2015.09.080
DO - 10.1016/j.athoracsur.2015.09.080
M3 - Article
C2 - 26675556
AN - SCOPUS:84949637916
SN - 0003-4975
VL - 101
SP - 1507
EP - 1513
JO - Annals of Thoracic Surgery
JF - Annals of Thoracic Surgery
IS - 4
ER -