Genetic polymorphisms at the rat and murine loci coding for dopamine D2-like receptors

Allelia W. Scott; Suzy A. Griffin; Robert R. Luedtke

doi:10.1016/0169-328X(94)00271-F

Genetic polymorphisms at the rat and murine loci coding for dopamine D2-like receptors

Allelia W. Scott, Suzy A. Griffin, Robert R. Luedtke

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6 Scopus citations

Abstract

Southern blot hybridization techniques have been used to identify genetic polymorphisms at the D2, D3 and D4 dopamine receptor loci in mice and rats. Genomic DNA from a panel of outbred and inbred strains of rats and inbred strains of mice was digested with a variety of restriction endonucleases. After separation of the restriction digests on the basis of size using agarose gel electrophoresis, ³²P-labeled DNA probes coding for the rat D2, D3 and D4 dopamine receptors were used to identify a series of genetic polymorphisms at each of these receptor loci. Genetic polymorphisms were found for the rat and murine D2, D3 and D4 dopamine receptor loci. It is anticipated that these genetic polymorphisms will be useful in pharmacogenetic studies to determine the influence of the D2-like receptors in reward and addictive behaviors.

Original language	English
Pages (from-to)	347-357
Number of pages	11
Journal	Molecular Brain Research
Volume	29
Issue number	2
DOIs	https://doi.org/10.1016/0169-328X(94)00271-F
State	Published - Apr 1995

Keywords

Dopamine receptor
Inbred strain
Restriction fragment length polymorphism
Southern blot hybridization

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10.1016/0169-328X(94)00271-F

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title = "Genetic polymorphisms at the rat and murine loci coding for dopamine D2-like receptors",

abstract = "Southern blot hybridization techniques have been used to identify genetic polymorphisms at the D2, D3 and D4 dopamine receptor loci in mice and rats. Genomic DNA from a panel of outbred and inbred strains of rats and inbred strains of mice was digested with a variety of restriction endonucleases. After separation of the restriction digests on the basis of size using agarose gel electrophoresis, 32P-labeled DNA probes coding for the rat D2, D3 and D4 dopamine receptors were used to identify a series of genetic polymorphisms at each of these receptor loci. Genetic polymorphisms were found for the rat and murine D2, D3 and D4 dopamine receptor loci. It is anticipated that these genetic polymorphisms will be useful in pharmacogenetic studies to determine the influence of the D2-like receptors in reward and addictive behaviors.",

keywords = "Dopamine receptor, Inbred strain, Restriction fragment length polymorphism, Southern blot hybridization",

author = "Scott, {Allelia W.} and Griffin, {Suzy A.} and Luedtke, {Robert R.}",

note = "Funding Information: The authors would like to acknowledge the technical assistance afforded to them by Dr. Arthur Eisenberg and Ms. Patricia Gibson of the DNA Identity Laboratory at the University of North Texas Health Science Center. We appreciate Dr. Gurnum Gill's assistance (Upjohn Co.) in making the genomic D4 receptor clone available to us prior to publication. We would like to thank Dr. Perry Molinoff and Dr. Frank George for their encouragement and constructive comments on this research. This research was supported in part by grants from the Scottish Rite Schizophrenia Research Program, the National Alliance for Research on Schizophrenia and Depression (NARSAD) and from the NINDS\]NS 30507).",

year = "1995",

month = apr,

doi = "10.1016/0169-328X(94)00271-F",

language = "English",

volume = "29",

pages = "347--357",

journal = "Molecular Brain Research",

issn = "0169-328X",

publisher = "Elsevier BV",

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T1 - Genetic polymorphisms at the rat and murine loci coding for dopamine D2-like receptors

AU - Scott, Allelia W.

AU - Griffin, Suzy A.

AU - Luedtke, Robert R.

N1 - Funding Information: The authors would like to acknowledge the technical assistance afforded to them by Dr. Arthur Eisenberg and Ms. Patricia Gibson of the DNA Identity Laboratory at the University of North Texas Health Science Center. We appreciate Dr. Gurnum Gill's assistance (Upjohn Co.) in making the genomic D4 receptor clone available to us prior to publication. We would like to thank Dr. Perry Molinoff and Dr. Frank George for their encouragement and constructive comments on this research. This research was supported in part by grants from the Scottish Rite Schizophrenia Research Program, the National Alliance for Research on Schizophrenia and Depression (NARSAD) and from the NINDS\]NS 30507).

PY - 1995/4

Y1 - 1995/4

N2 - Southern blot hybridization techniques have been used to identify genetic polymorphisms at the D2, D3 and D4 dopamine receptor loci in mice and rats. Genomic DNA from a panel of outbred and inbred strains of rats and inbred strains of mice was digested with a variety of restriction endonucleases. After separation of the restriction digests on the basis of size using agarose gel electrophoresis, 32P-labeled DNA probes coding for the rat D2, D3 and D4 dopamine receptors were used to identify a series of genetic polymorphisms at each of these receptor loci. Genetic polymorphisms were found for the rat and murine D2, D3 and D4 dopamine receptor loci. It is anticipated that these genetic polymorphisms will be useful in pharmacogenetic studies to determine the influence of the D2-like receptors in reward and addictive behaviors.

AB - Southern blot hybridization techniques have been used to identify genetic polymorphisms at the D2, D3 and D4 dopamine receptor loci in mice and rats. Genomic DNA from a panel of outbred and inbred strains of rats and inbred strains of mice was digested with a variety of restriction endonucleases. After separation of the restriction digests on the basis of size using agarose gel electrophoresis, 32P-labeled DNA probes coding for the rat D2, D3 and D4 dopamine receptors were used to identify a series of genetic polymorphisms at each of these receptor loci. Genetic polymorphisms were found for the rat and murine D2, D3 and D4 dopamine receptor loci. It is anticipated that these genetic polymorphisms will be useful in pharmacogenetic studies to determine the influence of the D2-like receptors in reward and addictive behaviors.

KW - Dopamine receptor

KW - Inbred strain

KW - Restriction fragment length polymorphism

KW - Southern blot hybridization

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AN - SCOPUS:0028907471

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VL - 29

SP - 347

EP - 357

JO - Molecular Brain Research

JF - Molecular Brain Research

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ER -

Genetic polymorphisms at the rat and murine loci coding for dopamine D2-like receptors

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Keywords

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