TY - JOUR
T1 - Genetic analysis of 7 medieval skeletons from the Aragonese Pyrenees
AU - Núnez, Carolina
AU - Sosa, Cecilia
AU - Baeta, Miriam
AU - Geppert, Maria
AU - Turnbough, Meredith
AU - Phillips, Nicole
AU - Casalod, Yolanda
AU - Bolea, Miguel
AU - Roby, Rhonda
AU - Budowle, Bruce
AU - Martínez-Jarreta, Begońa
N1 - Copyright:
Copyright 2012 Elsevier B.V., All rights reserved.
PY - 2011/6
Y1 - 2011/6
N2 - Aim: To perform a genetic characterization of 7 skeletons from medieval age found in a burial site in the Aragonese Pyrenees. Methods: Allele frequencies of autosomal short tandem repeats (STR) loci were determined by 3 different STR systems. Mitochondrial DNA (mtDNA) and Y-chromosome haplogroups were determined by sequencing of the hypervariable segment 1 of mtDNA and typing of phylogenetic Y chromosome single nucleotide polymorphisms (YSNP) markers, respectively. Possible familial relationships were also investigated. Results: Complete or partial STR profiles were obtained in 3 of the 7 samples. Mitochondrial DNA haplogroup was determined in 6 samples, with 5 of them corresponding to the haplogroup H and 1 to the haplogroup U5a. Ychromosome haplogroup was determined in 2 samples, corresponding to the haplogroup R. In one of them, the sub-branch R1b1b2 was determined. mtDNA sequences indicated that some of the individuals could be maternally related, while STR profiles indicated no direct family relationships. Conclusions: Despite the antiquity of the samples and great difficulty that genetic analyses entail, the combined use of autosomal STR markers, Y-chromosome informative SNPs, and mtDNA sequences allowed us to genotype a group of skeletons from the medieval age.
AB - Aim: To perform a genetic characterization of 7 skeletons from medieval age found in a burial site in the Aragonese Pyrenees. Methods: Allele frequencies of autosomal short tandem repeats (STR) loci were determined by 3 different STR systems. Mitochondrial DNA (mtDNA) and Y-chromosome haplogroups were determined by sequencing of the hypervariable segment 1 of mtDNA and typing of phylogenetic Y chromosome single nucleotide polymorphisms (YSNP) markers, respectively. Possible familial relationships were also investigated. Results: Complete or partial STR profiles were obtained in 3 of the 7 samples. Mitochondrial DNA haplogroup was determined in 6 samples, with 5 of them corresponding to the haplogroup H and 1 to the haplogroup U5a. Ychromosome haplogroup was determined in 2 samples, corresponding to the haplogroup R. In one of them, the sub-branch R1b1b2 was determined. mtDNA sequences indicated that some of the individuals could be maternally related, while STR profiles indicated no direct family relationships. Conclusions: Despite the antiquity of the samples and great difficulty that genetic analyses entail, the combined use of autosomal STR markers, Y-chromosome informative SNPs, and mtDNA sequences allowed us to genotype a group of skeletons from the medieval age.
UR - http://www.scopus.com/inward/record.url?scp=80051928027&partnerID=8YFLogxK
U2 - 10.3325/cmj.2011.52.336
DO - 10.3325/cmj.2011.52.336
M3 - Article
C2 - 21674829
AN - SCOPUS:80051928027
VL - 52
SP - 336
EP - 343
JO - Croatian Medical Journal
JF - Croatian Medical Journal
SN - 0353-9504
IS - 3
ER -