@inproceedings{05f2a09a59ec45858d98a05794ab0132,
title = "Generation of transgene-free iPSC lines from human normal and neoplastic blood cells using episomal vectors",
abstract = "Human induced pluripotent stem cells (iPSCs) have become an important tool for modeling human diseases and are considered a potential source of therapeutic cells. Original methods for iPSC generation use fibroblasts as a cell source for reprogramming and retroviral vectors as a delivery method of the reprogramming factors. However, fibroblasts require extended time for expansion and viral delivery of transgenes results in the integration of vector sequences into the genome which is a source of potential insertion mutagenesis, residual expressions, and reactivation of transgenes during differentiation. Here, we provide a detailed protocol for the efficient generation of transgene-free iPSC lines from human bone marrow and cord blood cells with a single transfection of non-integrating episomal plasmids. This method uses mononuclear bone marrow and cord blood cells, and makes it possible to generate transgene-free iPSCs 1-3 weeks faster than previous methods of reprogramming with fibroblasts. Additionally, we show that this approach can be used for efficient reprogramming of chronic myeloid leukemia cells.",
keywords = "Chronic myeloid leukemia, Cord blood, Episomal plasmids, Epstein-Barr virus, Human bone marrow, Induced pluripotent stem cells, Reprogramming",
author = "Kejin Hu and Igor Slukvin",
year = "2013",
doi = "10.1007/978-1-62703-348-0_13",
language = "English",
isbn = "9781627033473",
series = "Methods in Molecular Biology",
pages = "163--176",
editor = "Uma Lakshmipathy and Mohan Vemuri",
booktitle = "Pluripotent Stem Cells",
}