Gene structure of the murine NK cell receptor 2B4: Presence of two alternatively spliced isoforms with distinct cytoplasmic domains

Susan E. Stepp, John D. Schatzle, Michael Bennett, Vinay Kumar, Porunelloor Mathew

Research output: Contribution to journalArticle

61 Scopus citations

Abstract

The NK cell receptor 2B4 is expressed on the surface of all murine NK cells and a subset of T cells. Ligation of 2B4 with monoclonal antibodies increases target cell lysis and IFN-γ production. 2B4 is the high-affinity counter-receptor for CD48 in mice and humans. 2B4-L is a member of the CD2 subgroup of the immunoglobulin supergene family, which includes CD48, LFA-3, CD84, Ly9 and SLAM. Here we describe 2B4-S, a second 2B4 isoform, and the genomic structure of the 2B4 gene. 2B4-S is identical to the 5' end of 2B4-L, differing only at the 3' end, corresponding to a portion of the cytoplasmic domain and the 3' untranslated sequence. Both 2B4-L and 2B4-S are expressed on IL-2-activated NK cells. The genomic clone of 2B4 reveals that the two cDNA clones are products of alternative splicing. Since they differ only in a portion of the cytoplasmic domain, it is likely that they transduce different signals.

Original languageEnglish
Pages (from-to)2392-2399
Number of pages8
JournalEuropean Journal of Immunology
Volume29
Issue number8
DOIs
StatePublished - 1 Jan 1999

Keywords

  • 2B4 receptor
  • Alternative splicing
  • Gene expression
  • Gene structure
  • NK cell

Fingerprint Dive into the research topics of 'Gene structure of the murine NK cell receptor 2B4: Presence of two alternatively spliced isoforms with distinct cytoplasmic domains'. Together they form a unique fingerprint.

  • Cite this