TY - JOUR
T1 - Gene structure of the murine NK cell receptor 2B4
T2 - Presence of two alternatively spliced isoforms with distinct cytoplasmic domains
AU - Stepp, Susan E.
AU - Schatzle, John D.
AU - Bennett, Michael
AU - Kumar, Vinay
AU - Mathew, Porunelloor A.
PY - 1999
Y1 - 1999
N2 - The NK cell receptor 2B4 is expressed on the surface of all murine NK cells and a subset of T cells. Ligation of 2B4 with monoclonal antibodies increases target cell lysis and IFN-γ production. 2B4 is the high-affinity counter-receptor for CD48 in mice and humans. 2B4-L is a member of the CD2 subgroup of the immunoglobulin supergene family, which includes CD48, LFA-3, CD84, Ly9 and SLAM. Here we describe 2B4-S, a second 2B4 isoform, and the genomic structure of the 2B4 gene. 2B4-S is identical to the 5' end of 2B4-L, differing only at the 3' end, corresponding to a portion of the cytoplasmic domain and the 3' untranslated sequence. Both 2B4-L and 2B4-S are expressed on IL-2-activated NK cells. The genomic clone of 2B4 reveals that the two cDNA clones are products of alternative splicing. Since they differ only in a portion of the cytoplasmic domain, it is likely that they transduce different signals.
AB - The NK cell receptor 2B4 is expressed on the surface of all murine NK cells and a subset of T cells. Ligation of 2B4 with monoclonal antibodies increases target cell lysis and IFN-γ production. 2B4 is the high-affinity counter-receptor for CD48 in mice and humans. 2B4-L is a member of the CD2 subgroup of the immunoglobulin supergene family, which includes CD48, LFA-3, CD84, Ly9 and SLAM. Here we describe 2B4-S, a second 2B4 isoform, and the genomic structure of the 2B4 gene. 2B4-S is identical to the 5' end of 2B4-L, differing only at the 3' end, corresponding to a portion of the cytoplasmic domain and the 3' untranslated sequence. Both 2B4-L and 2B4-S are expressed on IL-2-activated NK cells. The genomic clone of 2B4 reveals that the two cDNA clones are products of alternative splicing. Since they differ only in a portion of the cytoplasmic domain, it is likely that they transduce different signals.
KW - 2B4 receptor
KW - Alternative splicing
KW - Gene expression
KW - Gene structure
KW - NK cell
UR - http://www.scopus.com/inward/record.url?scp=0032793466&partnerID=8YFLogxK
U2 - 10.1002/(SICI)1521-4141(199908)29:08<2392::AID-IMMU2392>3.0.CO;2-R
DO - 10.1002/(SICI)1521-4141(199908)29:08<2392::AID-IMMU2392>3.0.CO;2-R
M3 - Article
C2 - 10458751
AN - SCOPUS:0032793466
SN - 0014-2980
VL - 29
SP - 2392
EP - 2399
JO - European Journal of Immunology
JF - European Journal of Immunology
IS - 8
ER -