TY - JOUR
T1 - Gene expression profiles of HeLa cells impacted by hepatitis C virus non-structural protein NS4B
AU - Zheng, Yi
AU - Ye, Lin Bai
AU - Liu, Jing
AU - Jing, Wei
AU - Timani, Khalid A.
AU - Yang, Xiao Jun
AU - Yang, Fan
AU - Wang, Wei
AU - Gao, Bo
AU - Wu, Zhen Hui
PY - 2005
Y1 - 2005
N2 - By a cDNA array representing 2308 signal transduction-related genes, we studied the expression profiles of HeLa cells stably transfected by Hepatitis C virus nonstructural protein 4B (HCV-NS4B). The alterations of the expression of four genes were confirmed by real-time quantitative RT-PCR; and the aldo-keto reductase family 1, member C1 (AKR1C1) enzyme activity was detected in HCV-NS4B transiently transfected HeLa cells and Huh-7, a human hepatoma cell line. Of the 2,308 genes we examined, 34 were up-regulated and 56 were down-regulated. These 90 genes involved oncogenes, tumor suppressors, cell receptors, complements, adhesions, transcription and translation, cytoskeletion and cellular stress. The expression profiling suggested that multiple regulatory pathways were affected by HCV-NS4B directly or indirectly. And since these genes are related to carcinogenesis, host defense system and cell homeostatic mechanism, we can conclude that HCV-NS4B could play some important roles in the pathogenesis mechanism of HCV.
AB - By a cDNA array representing 2308 signal transduction-related genes, we studied the expression profiles of HeLa cells stably transfected by Hepatitis C virus nonstructural protein 4B (HCV-NS4B). The alterations of the expression of four genes were confirmed by real-time quantitative RT-PCR; and the aldo-keto reductase family 1, member C1 (AKR1C1) enzyme activity was detected in HCV-NS4B transiently transfected HeLa cells and Huh-7, a human hepatoma cell line. Of the 2,308 genes we examined, 34 were up-regulated and 56 were down-regulated. These 90 genes involved oncogenes, tumor suppressors, cell receptors, complements, adhesions, transcription and translation, cytoskeletion and cellular stress. The expression profiling suggested that multiple regulatory pathways were affected by HCV-NS4B directly or indirectly. And since these genes are related to carcinogenesis, host defense system and cell homeostatic mechanism, we can conclude that HCV-NS4B could play some important roles in the pathogenesis mechanism of HCV.
KW - AKR1C1
KW - Hepatitis C virus
KW - cDNA microarray
UR - http://www.scopus.com/inward/record.url?scp=20444445185&partnerID=8YFLogxK
U2 - 10.5483/bmbrep.2005.38.2.151
DO - 10.5483/bmbrep.2005.38.2.151
M3 - Article
C2 - 15826491
AN - SCOPUS:20444445185
SN - 1225-8687
VL - 38
SP - 151
EP - 160
JO - Journal of Biochemistry and Molecular Biology
JF - Journal of Biochemistry and Molecular Biology
IS - 2
ER -