Gene expression profiles of HeLa cells impacted by hepatitis C virus non-structural protein NS4B

Yi Zheng, Lin Bai Ye, Jing Liu, Wei Jing, Khalid A. Timani, Xiao Jun Yang, Fan Yang, Wei Wang, Bo Gao, Zhen Hui Wu

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Abstract

By a cDNA array representing 2308 signal transduction-related genes, we studied the expression profiles of HeLa cells stably transfected by Hepatitis C virus nonstructural protein 4B (HCV-NS4B). The alterations of the expression of four genes were confirmed by real-time quantitative RT-PCR; and the aldo-keto reductase family 1, member C1 (AKR1C1) enzyme activity was detected in HCV-NS4B transiently transfected HeLa cells and Huh-7, a human hepatoma cell line. Of the 2,308 genes we examined, 34 were up-regulated and 56 were down-regulated. These 90 genes involved oncogenes, tumor suppressors, cell receptors, complements, adhesions, transcription and translation, cytoskeletion and cellular stress. The expression profiling suggested that multiple regulatory pathways were affected by HCV-NS4B directly or indirectly. And since these genes are related to carcinogenesis, host defense system and cell homeostatic mechanism, we can conclude that HCV-NS4B could play some important roles in the pathogenesis mechanism of HCV.

Original languageEnglish
Pages (from-to)151-160
Number of pages10
JournalJournal of Biochemistry and Molecular Biology
Volume38
Issue number2
Publication statusPublished - 1 Dec 2005

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Keywords

  • AKR1C1
  • Hepatitis C virus
  • cDNA microarray

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