Gene expression profiles of HeLa cells impacted by hepatitis C virus non-structural protein NS4B

Yi Zheng, Lin Bai Ye, Jing Liu, Wei Jing, Khalid A. Timani, Xiao Jun Yang, Fan Yang, Wei Wang, Bo Gao, Zhen Hui Wu

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Abstract

By a cDNA array representing 2308 signal transduction-related genes, we studied the expression profiles of HeLa cells stably transfected by Hepatitis C virus nonstructural protein 4B (HCV-NS4B). The alterations of the expression of four genes were confirmed by real-time quantitative RT-PCR; and the aldo-keto reductase family 1, member C1 (AKR1C1) enzyme activity was detected in HCV-NS4B transiently transfected HeLa cells and Huh-7, a human hepatoma cell line. Of the 2,308 genes we examined, 34 were up-regulated and 56 were down-regulated. These 90 genes involved oncogenes, tumor suppressors, cell receptors, complements, adhesions, transcription and translation, cytoskeletion and cellular stress. The expression profiling suggested that multiple regulatory pathways were affected by HCV-NS4B directly or indirectly. And since these genes are related to carcinogenesis, host defense system and cell homeostatic mechanism, we can conclude that HCV-NS4B could play some important roles in the pathogenesis mechanism of HCV.

Original languageEnglish
Pages (from-to)151-160
Number of pages10
JournalJournal of Biochemistry and Molecular Biology
Volume38
Issue number2
StatePublished - 1 Dec 2005

Fingerprint

Viral Structural Proteins
Viruses
HeLa Cells
Transcriptome
Gene expression
Hepacivirus
Genes
Proteins
Complement Receptors
Oligonucleotide Array Sequence Analysis
Signal transduction
Oncogenes
Enzyme activity
Real-Time Polymerase Chain Reaction
Hepatocellular Carcinoma
Signal Transduction
Transcription
Carcinogenesis
Tumors
Gene Expression

Keywords

  • AKR1C1
  • Hepatitis C virus
  • cDNA microarray

Cite this

Zheng, Yi ; Ye, Lin Bai ; Liu, Jing ; Jing, Wei ; Timani, Khalid A. ; Yang, Xiao Jun ; Yang, Fan ; Wang, Wei ; Gao, Bo ; Wu, Zhen Hui. / Gene expression profiles of HeLa cells impacted by hepatitis C virus non-structural protein NS4B. In: Journal of Biochemistry and Molecular Biology. 2005 ; Vol. 38, No. 2. pp. 151-160.
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Zheng, Y, Ye, LB, Liu, J, Jing, W, Timani, KA, Yang, XJ, Yang, F, Wang, W, Gao, B & Wu, ZH 2005, 'Gene expression profiles of HeLa cells impacted by hepatitis C virus non-structural protein NS4B', Journal of Biochemistry and Molecular Biology, vol. 38, no. 2, pp. 151-160.

Gene expression profiles of HeLa cells impacted by hepatitis C virus non-structural protein NS4B. / Zheng, Yi; Ye, Lin Bai; Liu, Jing; Jing, Wei; Timani, Khalid A.; Yang, Xiao Jun; Yang, Fan; Wang, Wei; Gao, Bo; Wu, Zhen Hui.

In: Journal of Biochemistry and Molecular Biology, Vol. 38, No. 2, 01.12.2005, p. 151-160.

Research output: Contribution to journalArticleResearchpeer-review

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