TY - JOUR
T1 - Gender and age-related cell compositional differences in C57BL/6 murine adipose tissue stromal vascular fraction
AU - Frazier, Trivia
AU - Lee, Stephen
AU - Bowles, Annie
AU - Semon, Julie
AU - Bunnell, Bruce
AU - Wu, Xiying
AU - Gimble, Jeffrey
N1 - Funding Information:
This work was funded in part by funding from the National Institutes of Health (R21DK094254). JMG and XW are the co-owners, co-founders, and Chief Scientific Officer and Vice President for Research, respectively, at LaCell LLC, a
Funding Information:
HHS | National Institutes of Health (NIH) (R21DK094254).
Publisher Copyright:
© 2018, © 2018 Informa UK Limited, trading as Taylor & Francis.
PY - 2018/7/3
Y1 - 2018/7/3
N2 - Adipose tissue is now recognized as a functional organ that contains cellular heterogeneity and diversity within anatomical depots. The stromal vascular fraction (SVF) of adipose contains endothelial progenitors, fibroblasts, lymphocytes, monocyte/macrophages, pericytes, pre-adipocytes, and stromal/stem cells, among others. In recent years, there has been a growing appreciation of the influence of age and gender in the field of stem cell biology. Yet few studies have evaluated the influence of biological age or sex on either SVF cell heterogeneity or immunophenotype. To address this issue, the current study has compared the flow cytometric characteristics between murine SVF of inguinal (iWAT), epidydimal (eWAT), and brown (BAT) adipose tissue of male and female, as well as young (6–8 week) and middle-aged (8–12 month) male C57BL/6 mice. Murine gender comparisons revealed male iWAT expressed higher percentages of leukocyte and CD34 + ASC-like sub-populations than female iWAT. Murine age comparisons revealed younger male iWAT, eWAT, and BAT SVF all contained a significantly higher percentage of pre-adipocytes, HSC-like cells, CD25 − , and FoxP3 + T-regulatory cells compared to SVF from middle-aged male mice. These findings highlight the potential contribution of biological variables on adipose-derived cell applications and experimental outcomes.
AB - Adipose tissue is now recognized as a functional organ that contains cellular heterogeneity and diversity within anatomical depots. The stromal vascular fraction (SVF) of adipose contains endothelial progenitors, fibroblasts, lymphocytes, monocyte/macrophages, pericytes, pre-adipocytes, and stromal/stem cells, among others. In recent years, there has been a growing appreciation of the influence of age and gender in the field of stem cell biology. Yet few studies have evaluated the influence of biological age or sex on either SVF cell heterogeneity or immunophenotype. To address this issue, the current study has compared the flow cytometric characteristics between murine SVF of inguinal (iWAT), epidydimal (eWAT), and brown (BAT) adipose tissue of male and female, as well as young (6–8 week) and middle-aged (8–12 month) male C57BL/6 mice. Murine gender comparisons revealed male iWAT expressed higher percentages of leukocyte and CD34 + ASC-like sub-populations than female iWAT. Murine age comparisons revealed younger male iWAT, eWAT, and BAT SVF all contained a significantly higher percentage of pre-adipocytes, HSC-like cells, CD25 − , and FoxP3 + T-regulatory cells compared to SVF from middle-aged male mice. These findings highlight the potential contribution of biological variables on adipose-derived cell applications and experimental outcomes.
KW - C57BL/6 mice
KW - Stromal vascular fraction (SVF) cells
KW - adipose tissue
KW - age analysis
KW - cell composition
KW - gender analysis
KW - regenerative medicine
UR - http://www.scopus.com/inward/record.url?scp=85055196950&partnerID=8YFLogxK
U2 - 10.1080/21623945.2018.1460009
DO - 10.1080/21623945.2018.1460009
M3 - Article
C2 - 29882687
AN - SCOPUS:85055196950
SN - 2162-3945
VL - 7
SP - 183
EP - 189
JO - Adipocyte
JF - Adipocyte
IS - 3
ER -