TY - JOUR
T1 - Gα12/13- and Rho-dependent activation of phospholipase C-ε by lysophosphatidic acid and thrombin receptors
AU - Hains, Melinda D.
AU - Wing, Michele R.
AU - Maddileti, Savitri
AU - Siderovski, David P.
AU - Harden, T. Kendall
PY - 2006
Y1 - 2006
N2 - Because phospholipase C ε (PLC-ε) is activated by Gα12/13 and Rho family GTPases, we investigated whether these G proteins contribute to the increased inositol lipid hydrolysis observed in COS-7 cells after activation of certain G protein-coupled receptors. Stimulation of inositol lipid hydrolysis by endogenous lysophosphatidic acid (LPA) or thrombin receptors was markedly enhanced by the expression of PLC-ε. Expression of the LPA1 or PAR1 receptor increased inositol phosphate production in response to LPA or SFLLRN, respectively, and these agonist-stimulated responses were markedly enhanced by coexpression of PLC-ε. Both LPA1 and PAR1 receptor-mediated activation of PLC-ε was inhibited by coexpression of the regulator of G protein signaling (RGS) domain of p115RhoGEF, a GTPase-activating protein for Gα12/13 but not by expression of the RGS domain of GRK2, which inhibits Gαq signaling. In contrast, activation of the G q-coupled M1 muscarinic or P2Y2 purinergic receptor was neither enhanced by coexpression with PLC-ε nor inhibited by the RGS domain of p115RhoGEF but was blocked by expression of the RGS domain of GRK2. Expression of the Rho inhibitor C3 botulinum toxin did not affect LPA- or SFLLRN-stimulated inositol lipid hydrolysis in the absence of PLC-ε but completely prevented the PLC-ε-dependent increase in inositol phosphate accumulation. Likewise, C3 toxin blocked the PLC-ε-dependent stimulatory effects of the LPA1, LPA2, LPA3, or PAR1 receptor but had no effect on the agonist-promoted inositol phosphate response of the M1 or P2Y2 receptor. Moreover, PLC-ε-dependent stimulation of inositol phosphate accumulation by activation of the epidermal growth factor receptor, which involves Ras- but not Rho-mediated activation of the phospholipase, was unaffected by C3 toxin. These studies illustrate that specific LPA and thrombin receptors promote inositol lipid signaling via activation of Gα12/13 and Rho.
AB - Because phospholipase C ε (PLC-ε) is activated by Gα12/13 and Rho family GTPases, we investigated whether these G proteins contribute to the increased inositol lipid hydrolysis observed in COS-7 cells after activation of certain G protein-coupled receptors. Stimulation of inositol lipid hydrolysis by endogenous lysophosphatidic acid (LPA) or thrombin receptors was markedly enhanced by the expression of PLC-ε. Expression of the LPA1 or PAR1 receptor increased inositol phosphate production in response to LPA or SFLLRN, respectively, and these agonist-stimulated responses were markedly enhanced by coexpression of PLC-ε. Both LPA1 and PAR1 receptor-mediated activation of PLC-ε was inhibited by coexpression of the regulator of G protein signaling (RGS) domain of p115RhoGEF, a GTPase-activating protein for Gα12/13 but not by expression of the RGS domain of GRK2, which inhibits Gαq signaling. In contrast, activation of the G q-coupled M1 muscarinic or P2Y2 purinergic receptor was neither enhanced by coexpression with PLC-ε nor inhibited by the RGS domain of p115RhoGEF but was blocked by expression of the RGS domain of GRK2. Expression of the Rho inhibitor C3 botulinum toxin did not affect LPA- or SFLLRN-stimulated inositol lipid hydrolysis in the absence of PLC-ε but completely prevented the PLC-ε-dependent increase in inositol phosphate accumulation. Likewise, C3 toxin blocked the PLC-ε-dependent stimulatory effects of the LPA1, LPA2, LPA3, or PAR1 receptor but had no effect on the agonist-promoted inositol phosphate response of the M1 or P2Y2 receptor. Moreover, PLC-ε-dependent stimulation of inositol phosphate accumulation by activation of the epidermal growth factor receptor, which involves Ras- but not Rho-mediated activation of the phospholipase, was unaffected by C3 toxin. These studies illustrate that specific LPA and thrombin receptors promote inositol lipid signaling via activation of Gα12/13 and Rho.
UR - http://www.scopus.com/inward/record.url?scp=33745143631&partnerID=8YFLogxK
U2 - 10.1124/mol.105.017921
DO - 10.1124/mol.105.017921
M3 - Article
C2 - 16554409
AN - SCOPUS:33745143631
SN - 0026-895X
VL - 69
SP - 2068
EP - 2075
JO - Molecular Pharmacology
JF - Molecular Pharmacology
IS - 6
ER -