TY - JOUR
T1 - Functional analysis of the promoter of the phase-specific WH11 gene of Candida albicans
AU - Srikantha, Thyagarajan
AU - Chandrasekhar, Anand
AU - Soll, David R.
PY - 1995/3
Y1 - 1995/3
N2 - Candida albicans WO-1 switches spontaneously, frequently, and reversibly between a hemispherical white and a fiat gray (opaque) colony-forming phenotype. This transition affects a number of morphological and physiological parameters and involves the activation and deactivation of phase-specific genes. The WH11 gene is transcribed in the white but not the opaque phase. A chimeric WH11-firefly luciferase gene containing the 5' upstream region of WH11 was demonstrated to be under phase regulation regardless of the site of integration, and a series of promoter deletion constructs was used to delineate two white-phase-specific transcription activation domains. Gel retardation experiments with the individual distal or proximal domain and white-phase or opaque-phase protein extract demonstrated the formation of one distal white-phase-specific complex and two proximal white-phase-specific complexes. Specific subfragments were tested for their ability to compete with the entire domain in the formation of complexes with white-phase protein extract in order to map the proximal domain sequence involved in white-phase-specific complex formation. Our results indicate that white-phase-specific transcription of WH11 is positively regulated by trans- acting factors interacting with two cis-acting activation sequences in the WH11 promoter.
AB - Candida albicans WO-1 switches spontaneously, frequently, and reversibly between a hemispherical white and a fiat gray (opaque) colony-forming phenotype. This transition affects a number of morphological and physiological parameters and involves the activation and deactivation of phase-specific genes. The WH11 gene is transcribed in the white but not the opaque phase. A chimeric WH11-firefly luciferase gene containing the 5' upstream region of WH11 was demonstrated to be under phase regulation regardless of the site of integration, and a series of promoter deletion constructs was used to delineate two white-phase-specific transcription activation domains. Gel retardation experiments with the individual distal or proximal domain and white-phase or opaque-phase protein extract demonstrated the formation of one distal white-phase-specific complex and two proximal white-phase-specific complexes. Specific subfragments were tested for their ability to compete with the entire domain in the formation of complexes with white-phase protein extract in order to map the proximal domain sequence involved in white-phase-specific complex formation. Our results indicate that white-phase-specific transcription of WH11 is positively regulated by trans- acting factors interacting with two cis-acting activation sequences in the WH11 promoter.
UR - http://www.scopus.com/inward/record.url?scp=0028985204&partnerID=8YFLogxK
U2 - 10.1128/mcb.15.3.1797
DO - 10.1128/mcb.15.3.1797
M3 - Article
C2 - 7862169
AN - SCOPUS:0028985204
SN - 0270-7306
VL - 15
SP - 1797
EP - 1805
JO - Molecular and Cellular Biology
JF - Molecular and Cellular Biology
IS - 3
ER -