FRET study in oligopeptide-linked donor-acceptor system in PVA matrix

Sunil Shah; Wlodek Mandecki; Ji Li; Zygmunt Gryczynski; Julian Borejdo; Ignacy Gryczynski; Rafal Fudala

doi:10.1088/2050-6120/4/4/047002

FRET study in oligopeptide-linked donor-acceptor system in PVA matrix

Sunil Shah, Wlodek Mandecki, Ji Li, Zygmunt Gryczynski, Julian Borejdo, Ignacy Gryczynski, Rafal Fudala

Microbiology, Immunology & Genetics

Research output: Contribution to journal › Article › peer-review

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Abstract

An oligopeptide: Lys-Gly-Pro-Arg-Ser-Leu-Ser-Gly-Lys-NH2, cleaved specifically by a matrix metalloproteinase 9 (MMP-9) at the Ser-Leu bond, was labeled on the ε-NH2 groups of lysine with donor (5, 6 TAMRA) and acceptor (HiLyte647) dye. The donor control was a peptide labeled with 5, 6 TAMRA only on the C-terminal lysine, and the acceptor control was free HiLyte647. Following three products were studied by dissolving in 10% (w/w) poly(vinyl alcohol) and dried on glass slides forming 200 micron films. Absorption spectra of the films show full additivity of donor and acceptor absorptions. A strong Fluorescence Resonance Energy Transfer (FRET) with an efficiency of about 85% was observed in the fluorescence emission and excitation spectra. The lifetime of the donor was shorter and heterogeneous compared with the donor control.

Original language	English
Number of pages	1
Journal	Methods and Applications in Fluorescence
Volume	4
Issue number	4
DOIs	https://doi.org/10.1088/2050-6120/4/4/047002
State	Published - 13 Dec 2016

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title = "FRET study in oligopeptide-linked donor-acceptor system in PVA matrix",

abstract = "An oligopeptide: Lys-Gly-Pro-Arg-Ser-Leu-Ser-Gly-Lys-NH2, cleaved specifically by a matrix metalloproteinase 9 (MMP-9) at the Ser-Leu bond, was labeled on the ε-NH2 groups of lysine with donor (5, 6 TAMRA) and acceptor (HiLyte647) dye. The donor control was a peptide labeled with 5, 6 TAMRA only on the C-terminal lysine, and the acceptor control was free HiLyte647. Following three products were studied by dissolving in 10% (w/w) poly(vinyl alcohol) and dried on glass slides forming 200 micron films. Absorption spectra of the films show full additivity of donor and acceptor absorptions. A strong Fluorescence Resonance Energy Transfer (FRET) with an efficiency of about 85% was observed in the fluorescence emission and excitation spectra. The lifetime of the donor was shorter and heterogeneous compared with the donor control.",

author = "Sunil Shah and Wlodek Mandecki and Ji Li and Zygmunt Gryczynski and Julian Borejdo and Ignacy Gryczynski and Rafal Fudala",

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doi = "10.1088/2050-6120/4/4/047002",

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T1 - FRET study in oligopeptide-linked donor-acceptor system in PVA matrix

AU - Shah, Sunil

AU - Mandecki, Wlodek

AU - Li, Ji

AU - Gryczynski, Zygmunt

AU - Borejdo, Julian

AU - Gryczynski, Ignacy

AU - Fudala, Rafal

PY - 2016/12/13

Y1 - 2016/12/13

N2 - An oligopeptide: Lys-Gly-Pro-Arg-Ser-Leu-Ser-Gly-Lys-NH2, cleaved specifically by a matrix metalloproteinase 9 (MMP-9) at the Ser-Leu bond, was labeled on the ε-NH2 groups of lysine with donor (5, 6 TAMRA) and acceptor (HiLyte647) dye. The donor control was a peptide labeled with 5, 6 TAMRA only on the C-terminal lysine, and the acceptor control was free HiLyte647. Following three products were studied by dissolving in 10% (w/w) poly(vinyl alcohol) and dried on glass slides forming 200 micron films. Absorption spectra of the films show full additivity of donor and acceptor absorptions. A strong Fluorescence Resonance Energy Transfer (FRET) with an efficiency of about 85% was observed in the fluorescence emission and excitation spectra. The lifetime of the donor was shorter and heterogeneous compared with the donor control.

AB - An oligopeptide: Lys-Gly-Pro-Arg-Ser-Leu-Ser-Gly-Lys-NH2, cleaved specifically by a matrix metalloproteinase 9 (MMP-9) at the Ser-Leu bond, was labeled on the ε-NH2 groups of lysine with donor (5, 6 TAMRA) and acceptor (HiLyte647) dye. The donor control was a peptide labeled with 5, 6 TAMRA only on the C-terminal lysine, and the acceptor control was free HiLyte647. Following three products were studied by dissolving in 10% (w/w) poly(vinyl alcohol) and dried on glass slides forming 200 micron films. Absorption spectra of the films show full additivity of donor and acceptor absorptions. A strong Fluorescence Resonance Energy Transfer (FRET) with an efficiency of about 85% was observed in the fluorescence emission and excitation spectra. The lifetime of the donor was shorter and heterogeneous compared with the donor control.

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SN - 2050-6120

VL - 4

JO - Methods and applications in fluorescence

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IS - 4

ER -

FRET study in oligopeptide-linked donor-acceptor system in PVA matrix

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