TY - JOUR
T1 - FRET study in oligopeptide-linked donor-acceptor system in PVA matrix
AU - Shah, Sunil
AU - Mandecki, Wlodek
AU - Li, Ji
AU - Gryczynski, Zygmunt
AU - Borejdo, Julian
AU - Gryczynski, Ignacy
AU - Fudala, Rafal
PY - 2016/12/13
Y1 - 2016/12/13
N2 - An oligopeptide: Lys-Gly-Pro-Arg-Ser-Leu-Ser-Gly-Lys-NH2, cleaved specifically by a matrix metalloproteinase 9 (MMP-9) at the Ser-Leu bond, was labeled on the ε-NH2 groups of lysine with donor (5, 6 TAMRA) and acceptor (HiLyte647) dye. The donor control was a peptide labeled with 5, 6 TAMRA only on the C-terminal lysine, and the acceptor control was free HiLyte647. Following three products were studied by dissolving in 10% (w/w) poly(vinyl alcohol) and dried on glass slides forming 200 micron films. Absorption spectra of the films show full additivity of donor and acceptor absorptions. A strong Fluorescence Resonance Energy Transfer (FRET) with an efficiency of about 85% was observed in the fluorescence emission and excitation spectra. The lifetime of the donor was shorter and heterogeneous compared with the donor control.
AB - An oligopeptide: Lys-Gly-Pro-Arg-Ser-Leu-Ser-Gly-Lys-NH2, cleaved specifically by a matrix metalloproteinase 9 (MMP-9) at the Ser-Leu bond, was labeled on the ε-NH2 groups of lysine with donor (5, 6 TAMRA) and acceptor (HiLyte647) dye. The donor control was a peptide labeled with 5, 6 TAMRA only on the C-terminal lysine, and the acceptor control was free HiLyte647. Following three products were studied by dissolving in 10% (w/w) poly(vinyl alcohol) and dried on glass slides forming 200 micron films. Absorption spectra of the films show full additivity of donor and acceptor absorptions. A strong Fluorescence Resonance Energy Transfer (FRET) with an efficiency of about 85% was observed in the fluorescence emission and excitation spectra. The lifetime of the donor was shorter and heterogeneous compared with the donor control.
UR - http://www.scopus.com/inward/record.url?scp=85054431123&partnerID=8YFLogxK
U2 - 10.1088/2050-6120/4/4/047002
DO - 10.1088/2050-6120/4/4/047002
M3 - Article
C2 - 28192309
AN - SCOPUS:85054431123
SN - 2050-6120
VL - 4
JO - Methods and applications in fluorescence
JF - Methods and applications in fluorescence
IS - 4
ER -