FRET enhanced fluorescent nanodiamonds

Rafal Fudala, Sangram Limbaji Raut, Badri P. Maliwal, T. W. Zerda, Ignacy Gryczynski, Eric Simanek, Julian Borejdo, Ryan Rich, Irina Akopova, Zygmunt Gryczynski

Research output: Contribution to journalArticleResearchpeer-review

9 Citations (Scopus)

Abstract

Fluorescent nanodiamonds (FNDs) are one of the new and very promising biocompatible nanomaterials that can be used both as a fluorescence imaging agent and a highly versatile platform for controlled functionalization to target and deliver a wide spectrum of therapeutic agents. Among the remarkable fluorescence properties are excellent photostability, emission between 600-700nm, quantum yield of 1 and moderately long fluorescence lifetimes. However the low absorption cross section of fluorescent (N-V)-centers limits FNDs' brightness. In this work we show that an approach based on the Forster resonance energy transfer (FRET) may significantly enhance the fluorescence signal observed from a single ND. We demonstrate that organic dyes (fluorophores) attached to the FND surface can efficiently transfer the excitation energy to (N-V)-centers. Multiple dyes positioned in close proximity to the ND facile surface may serve as harvesting antennas transferring excitation energy to the fluorescent centers. We propose that, with the help of some of the functional groups present on the FND surface, we can either directly link flurophores or use scalable dendrimer chemistry to position many organic dyes at a calibrated distance. Also, the remaining multiple functional groups will be still available for particle targeting and drug delivery. This opens a new way for designing a new type of theranostics particles of ultrahigh brightness, high photostability, specific targeting, and high capacity for drug delivery.

Original languageEnglish
Pages (from-to)1127-1133
Number of pages7
JournalCurrent Pharmaceutical Biotechnology
Volume14
Issue number13
DOIs
StatePublished - 1 Jan 2014

Fingerprint

Nanodiamonds
Fluorescence Resonance Energy Transfer
Coloring Agents
Fluorescence
Dendrimers
Nanostructures
Optical Imaging
Energy Transfer
Drug Delivery Systems
Fluorescent Dyes
Pharmaceutical Preparations

Keywords

  • Fluorescence
  • Fluorescence nanodiamonds
  • Forester resonance energy transfer (FRET)

Cite this

Fudala, Rafal ; Raut, Sangram Limbaji ; Maliwal, Badri P. ; Zerda, T. W. ; Gryczynski, Ignacy ; Simanek, Eric ; Borejdo, Julian ; Rich, Ryan ; Akopova, Irina ; Gryczynski, Zygmunt. / FRET enhanced fluorescent nanodiamonds. In: Current Pharmaceutical Biotechnology. 2014 ; Vol. 14, No. 13. pp. 1127-1133.
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FRET enhanced fluorescent nanodiamonds. / Fudala, Rafal; Raut, Sangram Limbaji; Maliwal, Badri P.; Zerda, T. W.; Gryczynski, Ignacy; Simanek, Eric; Borejdo, Julian; Rich, Ryan; Akopova, Irina; Gryczynski, Zygmunt.

In: Current Pharmaceutical Biotechnology, Vol. 14, No. 13, 01.01.2014, p. 1127-1133.

Research output: Contribution to journalArticleResearchpeer-review

TY - JOUR

T1 - FRET enhanced fluorescent nanodiamonds

AU - Fudala, Rafal

AU - Raut, Sangram Limbaji

AU - Maliwal, Badri P.

AU - Zerda, T. W.

AU - Gryczynski, Ignacy

AU - Simanek, Eric

AU - Borejdo, Julian

AU - Rich, Ryan

AU - Akopova, Irina

AU - Gryczynski, Zygmunt

PY - 2014/1/1

Y1 - 2014/1/1

N2 - Fluorescent nanodiamonds (FNDs) are one of the new and very promising biocompatible nanomaterials that can be used both as a fluorescence imaging agent and a highly versatile platform for controlled functionalization to target and deliver a wide spectrum of therapeutic agents. Among the remarkable fluorescence properties are excellent photostability, emission between 600-700nm, quantum yield of 1 and moderately long fluorescence lifetimes. However the low absorption cross section of fluorescent (N-V)-centers limits FNDs' brightness. In this work we show that an approach based on the Forster resonance energy transfer (FRET) may significantly enhance the fluorescence signal observed from a single ND. We demonstrate that organic dyes (fluorophores) attached to the FND surface can efficiently transfer the excitation energy to (N-V)-centers. Multiple dyes positioned in close proximity to the ND facile surface may serve as harvesting antennas transferring excitation energy to the fluorescent centers. We propose that, with the help of some of the functional groups present on the FND surface, we can either directly link flurophores or use scalable dendrimer chemistry to position many organic dyes at a calibrated distance. Also, the remaining multiple functional groups will be still available for particle targeting and drug delivery. This opens a new way for designing a new type of theranostics particles of ultrahigh brightness, high photostability, specific targeting, and high capacity for drug delivery.

AB - Fluorescent nanodiamonds (FNDs) are one of the new and very promising biocompatible nanomaterials that can be used both as a fluorescence imaging agent and a highly versatile platform for controlled functionalization to target and deliver a wide spectrum of therapeutic agents. Among the remarkable fluorescence properties are excellent photostability, emission between 600-700nm, quantum yield of 1 and moderately long fluorescence lifetimes. However the low absorption cross section of fluorescent (N-V)-centers limits FNDs' brightness. In this work we show that an approach based on the Forster resonance energy transfer (FRET) may significantly enhance the fluorescence signal observed from a single ND. We demonstrate that organic dyes (fluorophores) attached to the FND surface can efficiently transfer the excitation energy to (N-V)-centers. Multiple dyes positioned in close proximity to the ND facile surface may serve as harvesting antennas transferring excitation energy to the fluorescent centers. We propose that, with the help of some of the functional groups present on the FND surface, we can either directly link flurophores or use scalable dendrimer chemistry to position many organic dyes at a calibrated distance. Also, the remaining multiple functional groups will be still available for particle targeting and drug delivery. This opens a new way for designing a new type of theranostics particles of ultrahigh brightness, high photostability, specific targeting, and high capacity for drug delivery.

KW - Fluorescence

KW - Fluorescence nanodiamonds

KW - Forester resonance energy transfer (FRET)

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DO - 10.2174/138920101413140605110711

M3 - Article

VL - 14

SP - 1127

EP - 1133

JO - Current Pharmaceutical Biotechnology

JF - Current Pharmaceutical Biotechnology

SN - 1389-2010

IS - 13

ER -