Abstract
Elevated hyaluronidase levels are found in the urine of bladder and prostate cancer patients. Therefore, HA-ase is regarded as an important biomarker for the detection of these cancers. In this report, we use a FRET based ratiometric sensing approach to detect the level of HA-ase in synthetic urine. For this, we have used a HA-FRET probe (hyaluronan) labeled with fluorescein as a donor and rhodamine as an acceptor. We monitor the digestion of our HA-FRET probe with different concentrations of HA-ase in synthetic urine via fluorescence emission. The extent to which FRET is released depends on the concentration of HA-ase. Our fluorescence intensity results are also supported with time resolved fluorescence decay data. This assay can be used to develop a non-invasive technique for the detection of bladder and/or prostate cancer progression.
Original language | English |
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Pages (from-to) | 470-474 |
Number of pages | 5 |
Journal | Current Pharmaceutical Biotechnology |
Volume | 14 |
Issue number | 4 |
DOIs | |
State | Published - 2013 |
Keywords
- Bladder cancer
- HA-FRET
- Hyaluronidase
- Ratiometric sensing