Frequency-domain fluorescence spectroscopy, a new method for the resolution of complex fluorescence emission

Joseph R. Lakowicz, Ignacy Gryczynski, Henryk Cherek, Gabor Laczko, Nanda Joshi

Research output: Contribution to journalShort surveypeer-review

1 Scopus citations

Abstract

The fluorescence emission from complex chemical and biological samples can be resolved by measuring the frequency-response of the emission, which is now possible from 1 to 2000 MHz. The frequency-response allows determination of the components in a mixture, construction of time-resolved emission spectra, and measurement of the dynamic and hydrodynamic properties of biological macromolecules. The instrumentation is relatively simple, and data acquisition times can be short. At present, this method may be superior to direct measurements of time-resolved fluorescence emission.

Original languageEnglish
Pages (from-to)257-263
Number of pages7
JournalTrends in Analytical Chemistry
Volume5
Issue number10
DOIs
StatePublished - 1986

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