TY - JOUR
T1 - Frequency-domain fluorescence spectroscopy, a new method for the resolution of complex fluorescence emission
AU - Lakowicz, Joseph R.
AU - Gryczynski, Ignacy
AU - Cherek, Henryk
AU - Laczko, Gabor
AU - Joshi, Nanda
N1 - Funding Information:
Acknowledgements This work wass upportedb y a grantD AAG29-85-G-0017 from the Army Research Office, grants PCM-8210878a nd DMB-08502835f rom the National Science Foundation and grants GM-29318 and GM-35154 from the National Institutes of Health. J.R.L. offers his special thanks to the National Science Foundation, for their support of the frequency-domain method when others thought the techniquec ould not be useful, and to Dr. Shinoda (Hamamatsu)f or donating the microchannelp late PMT. I.G. expressesa ppreciationf or financial support from CPBP 01.06.2.01a nd H.C. for support from CPBP 01.06.2.03(P oland).
PY - 1986
Y1 - 1986
N2 - The fluorescence emission from complex chemical and biological samples can be resolved by measuring the frequency-response of the emission, which is now possible from 1 to 2000 MHz. The frequency-response allows determination of the components in a mixture, construction of time-resolved emission spectra, and measurement of the dynamic and hydrodynamic properties of biological macromolecules. The instrumentation is relatively simple, and data acquisition times can be short. At present, this method may be superior to direct measurements of time-resolved fluorescence emission.
AB - The fluorescence emission from complex chemical and biological samples can be resolved by measuring the frequency-response of the emission, which is now possible from 1 to 2000 MHz. The frequency-response allows determination of the components in a mixture, construction of time-resolved emission spectra, and measurement of the dynamic and hydrodynamic properties of biological macromolecules. The instrumentation is relatively simple, and data acquisition times can be short. At present, this method may be superior to direct measurements of time-resolved fluorescence emission.
UR - http://www.scopus.com/inward/record.url?scp=0022812277&partnerID=8YFLogxK
U2 - 10.1016/0165-9936(86)85075-0
DO - 10.1016/0165-9936(86)85075-0
M3 - Short survey
AN - SCOPUS:0022812277
SN - 0165-9936
VL - 5
SP - 257
EP - 263
JO - Trends in Analytical Chemistry
JF - Trends in Analytical Chemistry
IS - 10
ER -