Focal ischemia induces expression of the DNA damage-inducible gene GADD45 in the rat brain

Kunlin Jin, Jun Chen, Kenji Kawaguchi, Raymond L. Zhu, R. Anne Stetler, Roger P. Simon, Steven H. Graham

Research output: Contribution to journalArticlepeer-review

52 Scopus citations

Abstract

GADD45 is a DNA damage-inducible gene that accelerates DNA excision repair and can be induced by a variety of DNA-damaging stimuli in mammalian cells. We investigated the expression of GADD45 mRNA and protein using in situ hybridization and immunocytochemistry in rat brains after 2 h of temporal focal ischemia. The expression of GADD45 mRNA was induced in neurons throughout ischemic cortex 4 h after the onset of ischemia but was restricted to ischemic penumbra regions at 24 h after ischemia. The expression of GADD45 protein was increased only in sublethally injured neurons in the penumbra regions at both 4 h and 24 h following ischemia. These results suggest that GADD45 could have a protective role in ischemic neurons.

Original languageEnglish
Pages (from-to)1797-1802
Number of pages6
JournalNeuroReport
Volume7
Issue number11
DOIs
StatePublished - 1996

Keywords

  • DNA damage
  • DNA repair
  • GADD45
  • cerebral ischemia and reperfusion
  • gene

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