Fluoride-induced changes in renal papillary cyclic-AMP

J. A. Maxwell, J. L. Caffrey, Thomas Yorio, R. J. Sinclair

Research output: Contribution to journalArticle

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Abstract

Studies were conducted which examined urinary excretion and papillary production of cyclic-AMP in the rat following vasopressin (ADH) stimulation in the presence or absence of inorganic fluoride ion (F). In one set of experiments, six anesthetized Fischer 344 rats were administered 5 munits arginine vasopressin during iv saline and sodium fluoride (NaF) infusions. Urinary cyclic-AMP concentration was unchanged by ADH but declined during NaF infusion, while urinary cyclic-AMP excretion rate was unchanged by ADH or ADH NaF. In a second set of experiments, 30 rats were divided into six groups of 5 each, and renal papilla and cortex were analyzed for cyclic-AMP. Group I was decapitated and not otherwise surgically manipulated. A second group had iv saline for 90 min while a third group had iv saline and ADH. A fourth group had isotonic NaF iv for 90 min and a fifth group had NaF and ADH. The results indicate that papillary cyclic-AMP is significantly increased by both ADH and NaF doses which did not cause changes in urinary cyclic-AMP excretion rates. We conclude from these experiments that determination of urinary cyclic-AMP concentration is not the best measure of vasopressin action and that tubular sensitivity to the hormone in the presence of F was better demonstrated by changes in papillary cyclic-AMP concentration. Further, it appears that the site of the biochemical renal lesion caused by F is at a site beyond the generation of cyclic-AMP.

Original languageEnglish
Pages (from-to)138-142
Number of pages5
JournalToxicology and Applied Pharmacology
Volume69
Issue number1
DOIs
StatePublished - 15 Jun 1983

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Fluorides
Cyclic AMP
Kidney
Rats
Vasopressins
Sodium Fluoride
Arginine Vasopressin
Experiments
Inbred F344 Rats
Hormones
Ions

Cite this

Maxwell, J. A. ; Caffrey, J. L. ; Yorio, Thomas ; Sinclair, R. J. / Fluoride-induced changes in renal papillary cyclic-AMP. In: Toxicology and Applied Pharmacology. 1983 ; Vol. 69, No. 1. pp. 138-142.
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abstract = "Studies were conducted which examined urinary excretion and papillary production of cyclic-AMP in the rat following vasopressin (ADH) stimulation in the presence or absence of inorganic fluoride ion (F). In one set of experiments, six anesthetized Fischer 344 rats were administered 5 munits arginine vasopressin during iv saline and sodium fluoride (NaF) infusions. Urinary cyclic-AMP concentration was unchanged by ADH but declined during NaF infusion, while urinary cyclic-AMP excretion rate was unchanged by ADH or ADH NaF. In a second set of experiments, 30 rats were divided into six groups of 5 each, and renal papilla and cortex were analyzed for cyclic-AMP. Group I was decapitated and not otherwise surgically manipulated. A second group had iv saline for 90 min while a third group had iv saline and ADH. A fourth group had isotonic NaF iv for 90 min and a fifth group had NaF and ADH. The results indicate that papillary cyclic-AMP is significantly increased by both ADH and NaF doses which did not cause changes in urinary cyclic-AMP excretion rates. We conclude from these experiments that determination of urinary cyclic-AMP concentration is not the best measure of vasopressin action and that tubular sensitivity to the hormone in the presence of F was better demonstrated by changes in papillary cyclic-AMP concentration. Further, it appears that the site of the biochemical renal lesion caused by F is at a site beyond the generation of cyclic-AMP.",
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Fluoride-induced changes in renal papillary cyclic-AMP. / Maxwell, J. A.; Caffrey, J. L.; Yorio, Thomas; Sinclair, R. J.

In: Toxicology and Applied Pharmacology, Vol. 69, No. 1, 15.06.1983, p. 138-142.

Research output: Contribution to journalArticle

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