TY - JOUR
T1 - Fluorescent properties of antioxidant cysteine ABZ analogue
AU - Raut, Sangram Limbaji
AU - Heck, Amber
AU - Vishwanatha, Jamboor
AU - Sarkar, Pabak
AU - Mody, Avani
AU - Luchowski, Rafal
AU - Gryczynski, Zygmunt
AU - Gryczynski, Ignacy
N1 - Funding Information:
This work was supported by DOD Grant W81XWH-09-1-0406 and Texas ETF-CCFT (Z.G.).
PY - 2011/3/2
Y1 - 2011/3/2
N2 - The antioxidant properties of aminobenzamide cysteine (ABZ Cys) makes it a molecule that can potentially be used as a drug in oxidative stress related diseases and delivered in the form of a nanoparticles. Here we have studied the photo-physical properties of ABZ Cys, a fluorescent analogue of a popular antioxidant N-acetyl cysteine (NAC). We have compared ABZ Cys steady state and time-resolved fluorescence properties with its parent compounds anthranilic acid and anthranilamide in solution as well as in poly-vinyl alcohol (PVA) polymer films. ABZ Cys did not show any significant shift in absorption after entrapment in PVA film, but there was a shift towards shorter wavelengths in the emission peak compared to the phosphate buffer solution. Fluorescence lifetimes and quantum yields indicated a slight quenching of ABZ Cys fluorescence in comparison to the cysteine-less parent compounds. We also demonstrated that very low concentrations of ABZ Cys, such as 100 nM, are readily detected by a commercial spectrofluorometer. Hence we have established the possible use of ABZ Cys in biomedical applications.
AB - The antioxidant properties of aminobenzamide cysteine (ABZ Cys) makes it a molecule that can potentially be used as a drug in oxidative stress related diseases and delivered in the form of a nanoparticles. Here we have studied the photo-physical properties of ABZ Cys, a fluorescent analogue of a popular antioxidant N-acetyl cysteine (NAC). We have compared ABZ Cys steady state and time-resolved fluorescence properties with its parent compounds anthranilic acid and anthranilamide in solution as well as in poly-vinyl alcohol (PVA) polymer films. ABZ Cys did not show any significant shift in absorption after entrapment in PVA film, but there was a shift towards shorter wavelengths in the emission peak compared to the phosphate buffer solution. Fluorescence lifetimes and quantum yields indicated a slight quenching of ABZ Cys fluorescence in comparison to the cysteine-less parent compounds. We also demonstrated that very low concentrations of ABZ Cys, such as 100 nM, are readily detected by a commercial spectrofluorometer. Hence we have established the possible use of ABZ Cys in biomedical applications.
KW - ABZ Cys
KW - Anthranilamide
KW - Anthranilic acid
KW - Fluorescence anisotropy
KW - Fluorescence lifetime
UR - http://www.scopus.com/inward/record.url?scp=79551506131&partnerID=8YFLogxK
U2 - 10.1016/j.jphotobiol.2010.12.012
DO - 10.1016/j.jphotobiol.2010.12.012
M3 - Article
C2 - 21237671
AN - SCOPUS:79551506131
SN - 1011-1344
VL - 102
SP - 241
EP - 245
JO - Journal of Photochemistry and Photobiology, B: Biology
JF - Journal of Photochemistry and Photobiology, B: Biology
IS - 3
ER -