Fluorescent properties of antioxidant cysteine ABZ analogue

Sangram Raut, Amber Heck, Jamboor Vishwanatha, Pabak Sarkar, Avani Mody, Rafal Luchowski, Zygmunt Gryczynski, Ignacy Gryczynski

Research output: Contribution to journalArticle

2 Scopus citations

Abstract

The antioxidant properties of aminobenzamide cysteine (ABZ Cys) makes it a molecule that can potentially be used as a drug in oxidative stress related diseases and delivered in the form of a nanoparticles. Here we have studied the photo-physical properties of ABZ Cys, a fluorescent analogue of a popular antioxidant N-acetyl cysteine (NAC). We have compared ABZ Cys steady state and time-resolved fluorescence properties with its parent compounds anthranilic acid and anthranilamide in solution as well as in poly-vinyl alcohol (PVA) polymer films. ABZ Cys did not show any significant shift in absorption after entrapment in PVA film, but there was a shift towards shorter wavelengths in the emission peak compared to the phosphate buffer solution. Fluorescence lifetimes and quantum yields indicated a slight quenching of ABZ Cys fluorescence in comparison to the cysteine-less parent compounds. We also demonstrated that very low concentrations of ABZ Cys, such as 100 nM, are readily detected by a commercial spectrofluorometer. Hence we have established the possible use of ABZ Cys in biomedical applications.

Original languageEnglish
Pages (from-to)241-245
Number of pages5
JournalJournal of Photochemistry and Photobiology B: Biology
Volume102
Issue number3
DOIs
StatePublished - 2 Mar 2011

Keywords

  • ABZ Cys
  • Anthranilamide
  • Anthranilic acid
  • Fluorescence anisotropy
  • Fluorescence lifetime

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