Fluorescent multiplex analysis of nine STR loci: Spanish population data

Carmen Entrala; Miguel Lorente; Jose A. Lorente; J. Carlos Alvarez; Tamyra Moretti; Bruce Budowle; Enrique Villanueva

doi:10.1016/S0379-0738(98)00165-0

Fluorescent multiplex analysis of nine STR loci: Spanish population data

Carmen Entrala, Miguel Lorente, Jose A. Lorente, J. Carlos Alvarez, Tamyra Moretti, Bruce Budowle, Enrique Villanueva

Research output: Contribution to journal › Article

17 Scopus citations

Abstract

A total of 171 Caucasians living in Andalucia (southern Spain) have been typed for nine short tandem repeat (STR) loci by multiplex PCR amplification using a commercially available kit (Profiler Plus; Perkin-Elmer, Norwalk, CT, USA) and semi-automatic electrophoresis (ABI Prism 377 DNA Sequencer, Applied Biosystems, Foster City, CA, USA). The kit enables typing of the STR loci D3S1358, VWA, FGA, D5S818, D13S317, D7S820, D8S1179, D21S11, and D18S51. All loci, except D7S820, meet Hardy-Weinberg equilibrium. Because of the large number of loci that can be analyzed, the power of discrimination (PD) is greater than 0.99999, and the probability of exclusion (PE) reaches 0.99991 in our population sample. Copyright (C) 1998 Elsevier Science Ireland Ltd.

Original language	English
Pages (from-to)	179-183
Number of pages	5
Journal	Forensic Science International
Volume	98
Issue number	3
DOIs	https://doi.org/10.1016/S0379-0738(98)00165-0
State	Published - 21 Dec 1998

Keywords

Fluorescent
Forensic genetics
PCR
STR
Spanish population

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Entrala, C., Lorente, M., Lorente, J. A., Alvarez, J. C., Moretti, T., Budowle, B., & Villanueva, E. (1998). Fluorescent multiplex analysis of nine STR loci: Spanish population data. Forensic Science International, 98(3), 179-183. https://doi.org/10.1016/S0379-0738(98)00165-0

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title = "Fluorescent multiplex analysis of nine STR loci: Spanish population data",

abstract = "A total of 171 Caucasians living in Andalucia (southern Spain) have been typed for nine short tandem repeat (STR) loci by multiplex PCR amplification using a commercially available kit (Profiler Plus; Perkin-Elmer, Norwalk, CT, USA) and semi-automatic electrophoresis (ABI Prism 377 DNA Sequencer, Applied Biosystems, Foster City, CA, USA). The kit enables typing of the STR loci D3S1358, VWA, FGA, D5S818, D13S317, D7S820, D8S1179, D21S11, and D18S51. All loci, except D7S820, meet Hardy-Weinberg equilibrium. Because of the large number of loci that can be analyzed, the power of discrimination (PD) is greater than 0.99999, and the probability of exclusion (PE) reaches 0.99991 in our population sample. Copyright (C) 1998 Elsevier Science Ireland Ltd.",

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