TY - JOUR
T1 - Fluorescence spectral properties of cyanine dye-labeled DNA oligomers on surfaces coated with silver particles
AU - Malicka, Joanna
AU - Gryczynski, Ignacy
AU - Fang, Jiyu
AU - Lakowicz, Joseph R.
N1 - Funding Information:
This work was supported by the NIH, National Center for Research Resources, RR08119. The authors thank Dr. Michael L. Johnson for providing the software for calculation of the χ 2 R surface.
PY - 2003/6/15
Y1 - 2003/6/15
N2 - We examined the fluorescence spectral properties of DNA oligomers, labeled with Cy3 or Cy5, when bound to quartz surfaces coated with metallic silver particles. Prior to binding of labeled DNA the surfaces were treated with polylysine or 3-aminopropyl triethoxysilane or were coated with avidin for binding of biotinylated oligomers. The fluorescence intensities were increased an average of 8-fold on these surfaces. Despite the increased emission intensity, the photostability of the labeled DNA was the same or higher on the silver-coated surfaces than on the uncoated slides. The time-integrated intensities, that is the area under the intensity plots with continuous illumination, increased an average of 6-fold. In all cases the lifetimes were dramatically shortened on the silver particles, indicating an over 100-fold increase in the radiative decay rates. These results suggest the use of substrates containing silver particles for increased sensitivity of DNA detection on DNA arrays.
AB - We examined the fluorescence spectral properties of DNA oligomers, labeled with Cy3 or Cy5, when bound to quartz surfaces coated with metallic silver particles. Prior to binding of labeled DNA the surfaces were treated with polylysine or 3-aminopropyl triethoxysilane or were coated with avidin for binding of biotinylated oligomers. The fluorescence intensities were increased an average of 8-fold on these surfaces. Despite the increased emission intensity, the photostability of the labeled DNA was the same or higher on the silver-coated surfaces than on the uncoated slides. The time-integrated intensities, that is the area under the intensity plots with continuous illumination, increased an average of 6-fold. In all cases the lifetimes were dramatically shortened on the silver particles, indicating an over 100-fold increase in the radiative decay rates. These results suggest the use of substrates containing silver particles for increased sensitivity of DNA detection on DNA arrays.
UR - http://www.scopus.com/inward/record.url?scp=0012625589&partnerID=8YFLogxK
U2 - 10.1016/S0003-2697(03)00005-8
DO - 10.1016/S0003-2697(03)00005-8
M3 - Article
C2 - 12758251
AN - SCOPUS:0012625589
SN - 0003-2697
VL - 317
SP - 136
EP - 146
JO - Analytical Biochemistry
JF - Analytical Biochemistry
IS - 2
ER -