Fluorescence spectral properties of cyanine dye labeled DNA near metallic silver particles

Joanna Malicka; Ignacy Gryczynski; Badri P. Maliwal; Jiyu Fang; Joseph R. Lakowicz

doi:10.1002/bip.10301

Fluorescence spectral properties of cyanine dye labeled DNA near metallic silver particles

Joanna Malicka, Ignacy Gryczynski, Badri P. Maliwal, Jiyu Fang, Joseph R. Lakowicz

Research output: Contribution to journal › Article › peer-review

33 Scopus citations

Abstract

Recent studies have demonstrated theft silver metallic particles can increase the quantum yield and decrease the lifetimes of nearby fluorophores. These studies are extended to double stranded DNA oligomers labeled with N,N′-(dipropyl)-tetramethylindocarbocyanine (Cy3) or N,N-(dipropyl)-tetramethylindodicarbocyanine (Cy5). The proximity to silver particles increases the apparent quantum yields and decreases the lifetimes of the double helical DNA 23-mer labeled individually with Cy3 or Cy5. The decreased lifetimes are accompanied by apparently increased photostability of the labeled oligomers near silver particles. Because of spatial averaging across the sample these results are likely to significantly underestimate the effects of silver particles on labeled DNA localized at an optimal distance from the metallic surface. These results suggest that DNA arrays fabricated on substrates with silver particles can display increased sensitivity and photostability in the analysis of gene expression.

Original language	English
Pages (from-to)	96-104
Number of pages	9
Journal	Biopolymers - Biospectroscopy Section
Volume	72
Issue number	2
DOIs	https://doi.org/10.1002/bip.10301
State	Published - 27 Mar 2003

Keywords

Cyanine dye
DNA
Fluorescence
Silver particles
Spectral properties

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10.1002/bip.10301

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title = "Fluorescence spectral properties of cyanine dye labeled DNA near metallic silver particles",

abstract = "Recent studies have demonstrated theft silver metallic particles can increase the quantum yield and decrease the lifetimes of nearby fluorophores. These studies are extended to double stranded DNA oligomers labeled with N,N′-(dipropyl)-tetramethylindocarbocyanine (Cy3) or N,N-(dipropyl)-tetramethylindodicarbocyanine (Cy5). The proximity to silver particles increases the apparent quantum yields and decreases the lifetimes of the double helical DNA 23-mer labeled individually with Cy3 or Cy5. The decreased lifetimes are accompanied by apparently increased photostability of the labeled oligomers near silver particles. Because of spatial averaging across the sample these results are likely to significantly underestimate the effects of silver particles on labeled DNA localized at an optimal distance from the metallic surface. These results suggest that DNA arrays fabricated on substrates with silver particles can display increased sensitivity and photostability in the analysis of gene expression.",

keywords = "Cyanine dye, DNA, Fluorescence, Silver particles, Spectral properties",

author = "Joanna Malicka and Ignacy Gryczynski and Maliwal, {Badri P.} and Jiyu Fang and Lakowicz, {Joseph R.}",

year = "2003",

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doi = "10.1002/bip.10301",

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T1 - Fluorescence spectral properties of cyanine dye labeled DNA near metallic silver particles

AU - Malicka, Joanna

AU - Gryczynski, Ignacy

AU - Maliwal, Badri P.

AU - Fang, Jiyu

AU - Lakowicz, Joseph R.

PY - 2003/3/27

Y1 - 2003/3/27

N2 - Recent studies have demonstrated theft silver metallic particles can increase the quantum yield and decrease the lifetimes of nearby fluorophores. These studies are extended to double stranded DNA oligomers labeled with N,N′-(dipropyl)-tetramethylindocarbocyanine (Cy3) or N,N-(dipropyl)-tetramethylindodicarbocyanine (Cy5). The proximity to silver particles increases the apparent quantum yields and decreases the lifetimes of the double helical DNA 23-mer labeled individually with Cy3 or Cy5. The decreased lifetimes are accompanied by apparently increased photostability of the labeled oligomers near silver particles. Because of spatial averaging across the sample these results are likely to significantly underestimate the effects of silver particles on labeled DNA localized at an optimal distance from the metallic surface. These results suggest that DNA arrays fabricated on substrates with silver particles can display increased sensitivity and photostability in the analysis of gene expression.

AB - Recent studies have demonstrated theft silver metallic particles can increase the quantum yield and decrease the lifetimes of nearby fluorophores. These studies are extended to double stranded DNA oligomers labeled with N,N′-(dipropyl)-tetramethylindocarbocyanine (Cy3) or N,N-(dipropyl)-tetramethylindodicarbocyanine (Cy5). The proximity to silver particles increases the apparent quantum yields and decreases the lifetimes of the double helical DNA 23-mer labeled individually with Cy3 or Cy5. The decreased lifetimes are accompanied by apparently increased photostability of the labeled oligomers near silver particles. Because of spatial averaging across the sample these results are likely to significantly underestimate the effects of silver particles on labeled DNA localized at an optimal distance from the metallic surface. These results suggest that DNA arrays fabricated on substrates with silver particles can display increased sensitivity and photostability in the analysis of gene expression.

KW - Cyanine dye

KW - DNA

KW - Fluorescence

KW - Silver particles

KW - Spectral properties

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DO - 10.1002/bip.10301

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VL - 72

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JO - Biopolymers

JF - Biopolymers

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ER -

Fluorescence spectral properties of cyanine dye labeled DNA near metallic silver particles

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Keywords

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