Fluorescence quenching/enhancement surface assays: Signal manipulation using silver-coated gold nanoparticles

Evgenia G. Matveeva, Tanya Shtoyko, Ignacy Gryczynski, Irina Akopova, Zygmunt Gryczynski

Research output: Contribution to journalArticle

26 Citations (Scopus)

Abstract

Gold nanoparticles covalently attached to the indium tin oxide coated glass slide drastically quench fluorescence of a surface immunoassay (approximately 5-fold). Silver electrochemically deposited over the gold particles leads to fluorescence amplification: signal increases approximately 7-8 times if compared to the signal on gold particles not covered with silver. This phenomenon allows enhancing of the surface immunoassays utilizing both types of nanoparticles.

Original languageEnglish
Pages (from-to)85-90
Number of pages6
JournalChemical Physics Letters
Volume454
Issue number1-3
DOIs
StatePublished - 10 Mar 2008

Fingerprint

Silver
Gold
manipulators
Quenching
Assays
immunoassay
Fluorescence
quenching
silver
gold
Nanoparticles
fluorescence
nanoparticles
augmentation
chutes
indium oxides
tin oxides
Amplification
Glass
glass

Cite this

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Fluorescence quenching/enhancement surface assays : Signal manipulation using silver-coated gold nanoparticles. / Matveeva, Evgenia G.; Shtoyko, Tanya; Gryczynski, Ignacy; Akopova, Irina; Gryczynski, Zygmunt.

In: Chemical Physics Letters, Vol. 454, No. 1-3, 10.03.2008, p. 85-90.

Research output: Contribution to journalArticle

TY - JOUR

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AU - Matveeva, Evgenia G.

AU - Shtoyko, Tanya

AU - Gryczynski, Ignacy

AU - Akopova, Irina

AU - Gryczynski, Zygmunt

PY - 2008/3/10

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