Fluorescence polarization standard for near infrared spectroscopy and microscopy

Ratal Luchowski, Pabak Sarkar, Shashank Bharill, Gabor Laczko, Julian Borejdo, Zygmunt Gryczynski, Ignacy Gryczynski

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

We present studies of polarized absorption [linear dichroism (LD)] and fluorescence polarization of the styryl derivative (LDS 798) embedded in oriented poly(vinyl alcohol) (PVA) films. These films were oriented by progressive stretching up to eight folds. Both vertical and horizontal components of absorptions and fluorescence were measured and dichroic ratios were determined for different film stretching ratios. The dichroic ratio and fluorescence anisotropy values were analyzed as a function of PVA film stretching ratio by fitting according to the previously developed theory. For maximum stretching ratios, exceptionally high anisotropy (∼0.8) and polarization (∼0.9) values have been measured. The stretched films have high polarization values also for isotropic excitation in a wide spectral range (500-700 nm). Such films can be conveniently used as high polarization standards and we envision they will also have applications in near infrared (NIR) imaging microscopy, where they can be used for correcting an instrumental factor in polarization measurements.

Original languageEnglish
Pages (from-to)6257-6265
Number of pages9
JournalApplied Optics
Volume47
Issue number33
DOIs
StatePublished - 20 Nov 2008

Fingerprint

Near infrared spectroscopy
Microscopic examination
Fluorescence
infrared spectroscopy
Polarization
microscopy
fluorescence
Stretching
polarization
Anisotropy
anisotropy
Infrared imaging
Dichroism
dichroism
alcohols
Alcohols
Derivatives
excitation

Cite this

Luchowski, Ratal ; Sarkar, Pabak ; Bharill, Shashank ; Laczko, Gabor ; Borejdo, Julian ; Gryczynski, Zygmunt ; Gryczynski, Ignacy. / Fluorescence polarization standard for near infrared spectroscopy and microscopy. In: Applied Optics. 2008 ; Vol. 47, No. 33. pp. 6257-6265.
@article{21f351d8b27849858527f7acfeefc58d,
title = "Fluorescence polarization standard for near infrared spectroscopy and microscopy",
abstract = "We present studies of polarized absorption [linear dichroism (LD)] and fluorescence polarization of the styryl derivative (LDS 798) embedded in oriented poly(vinyl alcohol) (PVA) films. These films were oriented by progressive stretching up to eight folds. Both vertical and horizontal components of absorptions and fluorescence were measured and dichroic ratios were determined for different film stretching ratios. The dichroic ratio and fluorescence anisotropy values were analyzed as a function of PVA film stretching ratio by fitting according to the previously developed theory. For maximum stretching ratios, exceptionally high anisotropy (∼0.8) and polarization (∼0.9) values have been measured. The stretched films have high polarization values also for isotropic excitation in a wide spectral range (500-700 nm). Such films can be conveniently used as high polarization standards and we envision they will also have applications in near infrared (NIR) imaging microscopy, where they can be used for correcting an instrumental factor in polarization measurements.",
author = "Ratal Luchowski and Pabak Sarkar and Shashank Bharill and Gabor Laczko and Julian Borejdo and Zygmunt Gryczynski and Ignacy Gryczynski",
year = "2008",
month = "11",
day = "20",
doi = "10.1364/AO.47.006257",
language = "English",
volume = "47",
pages = "6257--6265",
journal = "Applied Optics",
issn = "1559-128X",
publisher = "The Optical Society",
number = "33",

}

Fluorescence polarization standard for near infrared spectroscopy and microscopy. / Luchowski, Ratal; Sarkar, Pabak; Bharill, Shashank; Laczko, Gabor; Borejdo, Julian; Gryczynski, Zygmunt; Gryczynski, Ignacy.

In: Applied Optics, Vol. 47, No. 33, 20.11.2008, p. 6257-6265.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Fluorescence polarization standard for near infrared spectroscopy and microscopy

AU - Luchowski, Ratal

AU - Sarkar, Pabak

AU - Bharill, Shashank

AU - Laczko, Gabor

AU - Borejdo, Julian

AU - Gryczynski, Zygmunt

AU - Gryczynski, Ignacy

PY - 2008/11/20

Y1 - 2008/11/20

N2 - We present studies of polarized absorption [linear dichroism (LD)] and fluorescence polarization of the styryl derivative (LDS 798) embedded in oriented poly(vinyl alcohol) (PVA) films. These films were oriented by progressive stretching up to eight folds. Both vertical and horizontal components of absorptions and fluorescence were measured and dichroic ratios were determined for different film stretching ratios. The dichroic ratio and fluorescence anisotropy values were analyzed as a function of PVA film stretching ratio by fitting according to the previously developed theory. For maximum stretching ratios, exceptionally high anisotropy (∼0.8) and polarization (∼0.9) values have been measured. The stretched films have high polarization values also for isotropic excitation in a wide spectral range (500-700 nm). Such films can be conveniently used as high polarization standards and we envision they will also have applications in near infrared (NIR) imaging microscopy, where they can be used for correcting an instrumental factor in polarization measurements.

AB - We present studies of polarized absorption [linear dichroism (LD)] and fluorescence polarization of the styryl derivative (LDS 798) embedded in oriented poly(vinyl alcohol) (PVA) films. These films were oriented by progressive stretching up to eight folds. Both vertical and horizontal components of absorptions and fluorescence were measured and dichroic ratios were determined for different film stretching ratios. The dichroic ratio and fluorescence anisotropy values were analyzed as a function of PVA film stretching ratio by fitting according to the previously developed theory. For maximum stretching ratios, exceptionally high anisotropy (∼0.8) and polarization (∼0.9) values have been measured. The stretched films have high polarization values also for isotropic excitation in a wide spectral range (500-700 nm). Such films can be conveniently used as high polarization standards and we envision they will also have applications in near infrared (NIR) imaging microscopy, where they can be used for correcting an instrumental factor in polarization measurements.

UR - http://www.scopus.com/inward/record.url?scp=58749083061&partnerID=8YFLogxK

U2 - 10.1364/AO.47.006257

DO - 10.1364/AO.47.006257

M3 - Article

C2 - 19023392

AN - SCOPUS:58749083061

VL - 47

SP - 6257

EP - 6265

JO - Applied Optics

JF - Applied Optics

SN - 1559-128X

IS - 33

ER -