FLUORESCENCE OF TYROSINE AND TRYPTOPHAN IN PROTEINS USING ONE‐ AND TWO‐PHOTON EXCITATION

Borys Kierdaszuk, Ignacy Gryczynski, Anna Modrak‐Wojcik, Agnieszka Bzowska, David Shugar, Joseph R. Lakowicz

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54 Scopus citations

Abstract

Abstract— We examined the emission spectra of tyrosine‐ and tryptophan‐containing proteins using one‐photon (270–310 nm) and two‐photon (565–610 nm) excitation. Emission spectra for two‐photon excitation of native and denatured human serum albumin and of three purine nucleoside phosphorylases indicated an absence of the tyrosine emission normally seen for one‐photon excitation below 290 nm. We examined the one‐photon and two‐photon excitation spectra of tyrosine‐tryptophan mixtures to determine the origin of selective excitation of the tryptophan residues. These results confirmed a short‐wavelength shift of the tyrosine two‐photon excitation spectrum relative to that of tryptophan, as recently reported by Rehms and Callis (1993) Chem. Phys. Lett. 208, 276–282.

Original languageEnglish
Pages (from-to)319-324
Number of pages6
JournalPhotochemistry and Photobiology
Volume61
Issue number4
DOIs
StatePublished - 1 Jan 1995

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