TY - JOUR
T1 - Fluorescence lifetime standards for time and frequency domain fluorescence spectroscopy
AU - Boens, Noël
AU - Qin, Wenwu
AU - Basarić, Nikola
AU - Hofkens, Johan
AU - Ameloot, Marcel
AU - Pouget, Jacques
AU - Lefèvre, Jean Pierre
AU - Valeur, Bernard
AU - Gratton, Enrico
AU - VandeVen, Martin
AU - Silva, Norberto D.
AU - Engelborghs, Yves
AU - Willaert, Katrien
AU - Sillen, Alain
AU - Rumbles, Garry
AU - Phillips, David
AU - Visser, Antonie J.W.G.
AU - Van Hoek, Arie
AU - Lakowicz, Joseph R.
AU - Malak, Henryk
AU - Gryczynski, Ignacy
AU - Szabo, Arthur G.
AU - Krajcarski, Don T.
AU - Tamai, Naoto
AU - Miura, Atsushi
PY - 2007/3/5
Y1 - 2007/3/5
N2 - A series of fluorophores with single-exponential fluorescence decays in liquid solution at 20 °C were measured independently by nine laboratories using single-photon timing and multifrequency phase and modulation fluorometry instruments with lasers as excitation source. The dyes that can serve as fluorescence lifetime standards for time-domain and frequency-domain measurements are all commercially available, are photostable under the conditions of the measurements, and are soluble in solvents of spectroscopic quality (methanol, cyclohexane, water). These lifetime standards are anthracene, 9-cyanoanthracene, 9,10-diphenylanthracene, N-methylcarbazole, coumarin 153, erythrosin B, N-acetyl-L-tryptophanamide, 1,4-bis(5-phenyloxazol-2-yl)benzene, 2,5-diphenyloxazole, rhodamine B, rubrene, AT-(3-sulfopropyl)acridinium, and 1,4-diphenylbenzene. At 20 °C, the fluorescence lifetimes vary from 89 ps to 31.2 ns, depending on fluorescent dye and solvent, which is a useful range for modern pico- and nanosecond time-domain or mega- to gigahertz frequency-domain instrumentation. The decay times are independent of the excitation and emission wavelengths. Dependent on the structure of the dye and the solvent, the excitation wavelengths used range from 284 to 575 nm, the emission from 330 to 630 nm. These lifetime standards may be used to either calibrate or test the resolution of time- and frequency-domain instrumentation or as reference compounds to eliminate the color effect in photomultiplier tubes. Statistical analyses by means of two-sample charts indicate that there is no laboratory bias in the lifetime determinations. Moreover, statistical tests show that there is an excellent correlation between the lifetimes estimated by the time-domain and frequency-domain fluorometries. Comprehensive tables compiling the results for 20 (fluorescence lifetime standard/solvent) combinations are given.
AB - A series of fluorophores with single-exponential fluorescence decays in liquid solution at 20 °C were measured independently by nine laboratories using single-photon timing and multifrequency phase and modulation fluorometry instruments with lasers as excitation source. The dyes that can serve as fluorescence lifetime standards for time-domain and frequency-domain measurements are all commercially available, are photostable under the conditions of the measurements, and are soluble in solvents of spectroscopic quality (methanol, cyclohexane, water). These lifetime standards are anthracene, 9-cyanoanthracene, 9,10-diphenylanthracene, N-methylcarbazole, coumarin 153, erythrosin B, N-acetyl-L-tryptophanamide, 1,4-bis(5-phenyloxazol-2-yl)benzene, 2,5-diphenyloxazole, rhodamine B, rubrene, AT-(3-sulfopropyl)acridinium, and 1,4-diphenylbenzene. At 20 °C, the fluorescence lifetimes vary from 89 ps to 31.2 ns, depending on fluorescent dye and solvent, which is a useful range for modern pico- and nanosecond time-domain or mega- to gigahertz frequency-domain instrumentation. The decay times are independent of the excitation and emission wavelengths. Dependent on the structure of the dye and the solvent, the excitation wavelengths used range from 284 to 575 nm, the emission from 330 to 630 nm. These lifetime standards may be used to either calibrate or test the resolution of time- and frequency-domain instrumentation or as reference compounds to eliminate the color effect in photomultiplier tubes. Statistical analyses by means of two-sample charts indicate that there is no laboratory bias in the lifetime determinations. Moreover, statistical tests show that there is an excellent correlation between the lifetimes estimated by the time-domain and frequency-domain fluorometries. Comprehensive tables compiling the results for 20 (fluorescence lifetime standard/solvent) combinations are given.
UR - http://www.scopus.com/inward/record.url?scp=33847620267&partnerID=8YFLogxK
U2 - 10.1021/ac062160k
DO - 10.1021/ac062160k
M3 - Article
C2 - 17269654
AN - SCOPUS:33847620267
SN - 0003-2700
VL - 79
SP - 2137
EP - 2149
JO - Analytical Chemistry
JF - Analytical Chemistry
IS - 5
ER -